platelet antibody 中文意思是什麼
platelet antibody
解釋
血小板抗體-
Changes of platelet antibody - igg level in patients with congestive splenomegaly and hypersplenism after different splenic operations
充血性脾大伴脾功能亢進患者不同脾切除術前後血小板相關抗體的變化 -
Platelet antibody changes before and after refractory idiopathic thrombocytopenic purpura treated with splenic artery embolization
難治性特發性血小板減少性紫癜脾動脈栓塞治療前後血小板抗體變化 -
Diagnosis value of detecting platelet associated antibody and platelet membrane glycoprotein for immune thrombocytopenia
血小板相關抗體及膜糖蛋白檢測在免疫性血小板減少癥患者診斷中的價值 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。
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