pmef 中文意思是什麼
pmef
解釋
小鼠胚胎成纖維細胞-
At primary culture, pgcs co - cultured with their gonadal stromall cells were well grown. when subculture, we used primary chicken embryonic fibroblast ( pcef ), primary mice embryonic fibroblast ( pmef ) and snl cells to make feeder cells. forward research founded that the pcef cells were the most suitable for the growth of putative eg cells when having various cytokines
繼代培養時，經過反復實驗比較雞胚原代成纖維細胞飼養層（ pcef ） 、小鼠原代成纖維細胞飼養層（ pmef ） 、 snl細胞飼養層等三種不同飼養層的作用效果，最終發現以雞胚原代成纖維細胞製作飼養層同時添加各種生長因子最適合雞類eg細胞的生長，但pcef與pmef之間的差異不顯著。 -
We also investigated the factors including feeder layer cells, growth factors and complements, which may affect the cultivation and differentiation of these cells in vitro. the main contents as follows : ( 1 ) isolation and culture of mouse embryonic fibroblast ( pmef ) and preparation the feeder layers
主要內容包括： （ 1 ）小鼠胚胎成纖維細胞（ mef ）的分離培養和飼養層細胞的製作以妊娠13 . 5天或14 16天的小鼠為材料，建立了mef分離培養體系。
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