pollen gene expression 中文意思是什麼

pollen gene expression 解釋
花粉基因表達
  • pollen : n. 1. 【植物；植物學】花粉。2. 【蟲類】粉面。vt. 授粉給；傳粉給；用花粉掩蓋。
  • gene : n. 【生物學】基因。 dominant gene顯性基因。
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  1. Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l. cv hetao flesh, which was cloned into plasmid vector pmd - 18 - t. the clon of antisense orientation were selected, and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw, antisence expression vector pbinya was constructed. at the base that pollination and fertilization of cucumis melo l. cv hetao was studied, using pollen tube pathway transformate cucumis melo l. cv hetao, 76 fruit had been obtained, moreover, hardness and content of sugar were analysed

    本實驗以河套蜜瓜果肉基因組dna為模板，用甜瓜acc氧化酶基因特異寡核苷酸鏈為引物進行pcr擴增，得到128bp的擴增產物。將得到的擴增產物克隆到質粒載體pmd - 18 - t上，篩選反向克隆，然後將其反向構建到植物表達載體pbinyxw的camv35s啟動子和tmv增強子「 」的下游，構建成反義表達載體pbinya 。並在對河套蜜瓜授粉受精生物學研究的基礎上，通過花粉管通道法轉化河套蜜瓜，共獲76顆瓜，並進行了硬度和含糖量的分析。
  2. The results obtained in our laboratory in the past decade years showed, apoplast calmodulin in plant kingdom may regulate a lot of growth and development process of plant, such as accelerating the proliferation of angelica dahurica suspension cells and the proplast cell regeneration, startup the pollen germination of many plants " pollen and accelerating the elongation of the pollen tube, stimulating the redox of corn root " s cell, inducing the expression of light independent rbss gene, and participating in the regulation of the restraining function of al ~ ( 3 + ) to pollen tube germination

    我室多年的研究結果表明，植物質外體cam可能做為多肽第一信使調節著植物體諸多的生長發育過程：如促進白芷懸浮細胞的細胞增殖和原生質體壁再生，啟動並促進多種花粉的萌發和花粉管的伸長，刺激玉米根細胞的氧化還原反應，誘導rbcs基因光不依賴的表達，以及參與調節胞外al ~ （ 3 + ）對花粉萌發的抑制效應等。
  3. Expression analysis the expression analysis of gharf in different tissues of cotton was carried out by rna dot blotting with ghakf probe labeled with dig. the result showed that the expression of arf gene was mainly in anthers of sterile and fertile, pollen of fertile, corolla rather than in leaves, radicel and ovule

    棉花ari ； 』基因的表達分析用地高辛將棉花arf基因標記為探針，與棉花洞a的不育株花藥、司有株花藥、可育花粉粒、葉片、花冠、胚根和胚珠的總rna進行斑點雜交。
  4. In order to establish the pollen - specific gene expression and regulation mechanism ( s ), it will be necessary to isolate and character more pollen - specific genes

    在對花器官發育的研究中，對雄蕊發育的研究由於與雄性不育密切相關而尤為引人注目。
  5. Histochemical gus assay showed that the gus staining was observed only in the mature pollen and germination pollen tube. there is no detectable gus activity in other floral organs, leaves and stems. these results suggested that st901 is a novel pollen - specific gene and the 288bp promoter fragment ( - 297 ? xfrom the translation start codon atg ) is sufficient for pollen - specific expression and os - element regulatory of st901 promoter was possibly concentrated in the region - 297 to - 9

    通過對gus酶活性的組織化學定位分析，表明， st901基因啟動子驅動gus基因特異地在成熟花粉和花粉管中表達， st901基因具有花粉表達特性；且288bp （ - 297至- 9 ） （ atg定為+ 1 ）的啟動子區段足以驅動gus基因在花粉中的特異表達， st901基因啟動子的花粉順式表達元件可能位於- 297至- 9之間。
  6. The result showed that both ldplcl and ldplc2 can physical interacted with gp, but neither ga nor g in addition, arabidopsis plc knock - out and overexpression lines were also characterized, and the result proved that lose of function of atplc ( at3g55940 ) induced atplc ( at3g08510 ) gene expression and increase pollen germination rate, whereas gain of function of atplc ( at3g55940 ) repressed atplc ( at3g08510 ) expression, and decrease pollen germination

    本文還利用酵母雙雜交體系檢驗了plc與g蛋白各亞基的相互作用，發現ldplci與gq和gy的雜交結果為陰性，與gp亞基可發生相互作用; ldplcz與gp弱相互作用，與其他亞基沒有作用。上述結果表明在g蛋白和plc的相互作用中， p亞基可能起調節作用。
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