polyketide 中文意思是什麼
polyketide
解釋
多聚乙酰-
In addition to avermectins, s. avermitilis produces oligomycin, a strongly toxic compound. gene deletion vector pxl05 was used to disrupt oligomycin polyketide synthase ( pks ) encoding genes ( olma ) in streptomyces avermitilis cz8 - 73, the producer of anthelmintic avermectins b and the cell growth inhibitor oligomycin. olma gene cluster in the chromosome was displaced by deletion allele on the plasmid via double crossover
本研究以產阿維菌素b和寡黴素的阿維鏈黴菌cz8 - 73為出發菌株，構建了基因缺失載體pxl05 ，並將其轉入cz8 - 73中，通過缺失載體和染色體之間的同源雙交換，對染色體上長達90kb的寡黴素聚酮合酶（ pks ）基因簇（ olma ）進行了缺失。 -
The results showed that the f fragment, 728bp in length, could be a new gene with a little homology to the genes coding for polyketide synthetase or fatty - acid synthetase and the b fragment, about 4kb in length, is inferred to have repeat sequences around tn5 insertion site, in which there is homology to the wa 314 right arm of the high - pathogeniciry island of yersinia enterocolitica. to reveal any pathogenicity of enterobacter cloacae b8 and its mutated strains b8b and b8f to animals, the experiment with mice was carried out
結果顯示， f片段長度為728bp ，與現有生物數據庫的blast比較分析，發現該序列僅有局部短於1oobp的區域與polyketide合成酶基因或與脂肪酸合成酶基因有低的同源性，推測為一新基因； b片段長約4kb ，序列拼接結果推測靠近tn5插入位點部位有重復序列，對b片段tn5遠端的部分序列進行blast比較，發現它與小腸結腸炎耶爾森氏菌的強毒力島有一定的同源性。 -
In consideration of the colinearity between the activities of avermectin pks domains and structure of the polyketide product, the dh2 domain of avermectin pks, which corresponds to c - 22, 23 dehydration, appears to have partial dehydratase activity and this results in a mixture of c - 22, 23 double bonds ( " 1 " components ) and c23 hydroxy compounds ( " 2 " components )
目前國內外尚未見有關僅產阿維菌素b1菌株的報道。根據阿維菌素pks基因結構與其聚酮合成反應步驟之間的線性關系，推測b2組分的產生可能是由於阿維菌素pks模塊2中脫水酶（ dh ）的不完全活性所造成。 -
Advances on polyketide biosynthesise by microorganism and the diversity of polyketide synthases
多聚酮的微生物合成及其多樣性研究進展
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