polymerase chain reaction-pcr 中文意思是什麼

polymerase chain reaction-pcr 解釋
聚合酶鏈反應
  • polymerase : n. 【生物化學】聚合酶。
  • chain : n 1 鏈子，鏈條；項圈；表鏈。2 連鎖；連續，一系列，一連串；（山）脈。3 〈常 pl 〉鐐銬；羈絆，拘束...
  • reaction : n 1 反作用，反應；反沖；反動力。2 【政治學】反動，倒退；復古（運動）。3 【化學】反應，【物理學】...
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  1. In this study, iltv - nm98a strain and iltv - wanggang strain were multiplied in chorioallantois. a pair of primers were devised according to the nucleic acid sequence of iltv tk gene and the dna of multiplied virus was used as pattern to amplify the gene of tk by polymerase chain reaction ( pcr ). the product of pcr was linked with suitable plasmid. then, the recombined plasmid was converted to escherichia coli. the converted escherichia coli

    根據已發表的iltvtk基因的核苷酸序列設計一對pcr引物，以增殖的兩株iltv的dna為模板，分別對它們的tk基因進行pcr擴增。將回收的pcr產物連接到適當的質粒載體上，轉化感受態大腸桿菌，通過篩選對iltvtk基因的陽性克隆進行擴增培養。
  2. Marek " s disease virus ( mdv ) phosphoprotein 24 ( pp24 ) gene was amplified from md11 strain by polymerase chain reaction ( pcr ). then we cloned it into the downstream of gst gene according to the right open reading frame ( orf ) in pgex - 6p - l vector

    本研究將型mdvmd11株的pp24基因的完整orf克隆入原核表達載體pgex - 6p - 1中，重組質粒pgex - pp24轉化bl21宿主菌后，經iptg誘導表達。
  3. In this paper, 45 e. coli strains isolated from chicken farms in sichuan province were determined to be the pathogenic e. coli by animal test. type 1 pili of 45 strains isolated was detected by msha. the pila gene of 45 avian pathogenic e. coli strains were amplified by the polymerase chain reaction ( pcr ) with primers designed according to the sequence of the pila gene in genbank. results showed that pcr was more sensitive, faster and more characteristic than msha to detect type 1 pili

    本研究將從四川規模化雞場分離鑒定、經1日齡雛雞致病性試驗得到的雞源致病性大腸桿菌45株，採用d -甘露糖敏感血凝試驗（ msha ）檢測1型菌毛，根據genbank中公布的人源大腸桿菌1型菌毛pila基因序列設計一對引物用pcr擴增雞源致病性大腸桿菌1型菌毛pila基因。
  4. We utilized microarray and real - time quantitatie polymerase chain reaction ( pcr ) to identify genes differentially expressed at 6 h reperfusion in periinfarct cortex from castrated rats with or without dht replacement

    我們利用微點陣和實時定量pcr方法在再灌注6小時時，對進行/未進行dht替代的閹割大鼠梗死周圍皮層的基因差異表達進行了鑒定。
  5. We utilized microarray and real - time quantitative polymerase chain reaction ( pcr ) to identify genes differentially expressed at 6 h reperfusion in periinfarct cortex from castrated rats with or without dht replacement

    我們利用微點陣和實時定量pcr方法在再灌注6小時時，對進行/未進行dht替代的閹割大鼠梗死周圍皮層的基因差異表達進行了鑒定。
  6. The percentage of polymorphic sites, degree of genetic polymorphism and genetic distance were compared and the phylogenetic tree was constructed by neighbor - joining method. the partial mitochondrial 16s rrna gene was amplified by polymerase chain reaction ( pcr ) and the pcr products were directly sequenced after purified. these sequences, together with the homologous sequences of another trichiuridae species lepidopus caudatus obtained from genbank were used to analyze nucleotide difference and to establish a upgma phylogenetic tree by means of biological informatics

    汝us價ay1830 ）各12個個體進行rapd分析，對比多態位點比例、遺傳多態度以及遺傳距離，並構建neighbor - join噸系統樹;通過pcr擴增出線粒體165rrna基因，純化后直接測序，利用生物信息學方法進行序列分析和核昔酸變異比較，結合ge紅bar止中大西洋叉尾帶魚（ lepid （護腳caud玫tuseuphrasen1788 ）同源序列構建u甲cm叭系統樹。
  7. By using single strand conformation polymorphism analysis of polymerase chain reaction ( pcr - sscp ), the frequency of codon 54 mutant allele of mbl structural gene in the population of hans was investigated. 3. the molecular beacon ( mb ) hybridization technique with visual monitoring was established

    建立聚合酶鏈反應單鏈構象多態性分析inglestrandconforma tlonpolymorphlsmanalysisofpol仰erasechainreactionproduction ， pcr sscp ）方法，初步調查漢族人群mbl基因ggc54gac突變情況。
  8. Polymerase chain reaction, pcr

    方法採用熱啟動聚合酶鏈反應
  9. Founder mice will be examined by polymerase chain reaction ( pcr ) and southern blot

    採用聚合酶鏈式反應（ pcr ）和southern印跡對陽性鼠進行檢測。
  10. A 40 - base polymorphic repeat sequence located in the 3 ' - untranslated region of the dat gene was purified and amplified by polymerase chain reaction ( pcr )

    將位於多巴胺運輸器基因3 '端未轉譯區段的40 -堿基多形性重復序列予以純化、經聚合酶鏈鎖反應放大。
  11. Purpose 1 construction of prokaryotic high expression vector of human platelet factor 4 ( h pf4 ) 2 expression and purification of r h pf4 3 bioassay of r h pf4 methods according to the modulation character of eukaryotic protein expression in prokaryotic cells, we design a pair of particular primers, and construct a prokaryotic expression vector pbv220 - r hpf4 by dna polymerase chain reaction ( pcr ) and dna recombinant technic. the expression plasmid was identified with pcr and dna sequencing. pbv220 - r hpf4 was transformed into e. coli dh5a, bl21 ( de3 ) and induced by increasing the temperature to 42. we identified the expression protein by sds - page and western - blotting

    目的1人血小板因子4 （ hpf4 ）原核高效表達克隆的構建2重組hpf4的表達及分離、純化工藝研究3重組hpf4的特性研究方法根據原核細胞表達真核蛋白的基因表達調控特點，設計合成一對特異引物，在pt7 - 7 - rpf4表達質粒的基礎上，應用聚合酶鏈式反應（ pcr ）對其cdna進行改造，通過dna重組技術構建成重組hpf4原核表達質粒pbv220 - rhpf4 ，用快速pcr檢測法、 dna測序分析，鑒定重組hpf4表達質粒的正確性。
  12. In order to use the responding ability to the inducers of pr - la promoter, two fragments, ip1 ( 900 bp ) and ips ( 603 bp ) were cloned from tobacco genomic dna by polymerase chain reaction ( pcr ) with primers designed according to the sequence reported in genbank. sequence analysis of the amplified 900 bp fragment indicated that the cloned sequence had 99 % of homology to the known sequence. its transcription start site, tata box and consensus sequence " tgac " conserved in pr genes were identical to those of pr - la promoter

    根據pr - 1a基因的報道序列，設計兩對引物，以煙草基因組為模板，通過pcr擴增得到900bp （ ipl ）和603bp （ ips ）兩個目標片段，序列測定表明克隆的啟動子與報道序列具有99的同源性，轉錄起始位點、 tatabox及保守序列tgac與報道序列均完全相同。
  13. In this experiment hcv structural gene was amplified by polymerase chain reaction ( pcr ), and was inserted into baculovirus expression vector pfastbacl to construct a recombinant transposing vector pfbl - cee. the plasmid pfb 1 - cee was transformed into dh1 obac competent e. coli cells. high molecular weight dna was prepared from the overnight cultures from the selected e. coli colonies, which was recombinant baculovirus shuttle vector containing hcv structural gene, named bac - cee

    本實驗用pcr擴增hcv結構區基因，克隆到桿狀病毒表達載體pfastbacl中，構建成重組轉座載體pfb1 - cee ，轉化dh10bac大腸桿菌感受態細胞，篩選陽性菌落，抽提大分子質粒dna ，獲得含hcv結構區基因的重組桿狀病毒穿梭載體bac - cee ，脂質體介導轉染sf9昆蟲細胞，出現細胞病變后，收集含有重組桿狀病毒顆粒的培養上消，重新感染sf9細胞，收集sf9細胞，進行12 . 5 sds -聚丙烯酰胺凝膠電泳，可見表達的蛋白條帶。
  14. The comparison of meq gene sequences of different pathotypes of marek ' s disease virus meq genes of different pathotypes of marek ' s disease virus ( mdv ) were amplified, in the whole opening reading frame ( orf ) by polymerase chain reaction ( pcr ) technique, sequenced and compared with the published sequence of ga strain ( representing vmdv ). cvi988 / rispens and 814, commercial vaccine strains popularly used worldwide and in china respectively ; 648a, representing very virulent plus ( vv + mdv ) and 6 field isolates, originated from guangxi commercial chickens with visceral lymphomas and vaccine breaks, representing high pathogenic ( hpmdv ) and two of them ( g2 and n ) were proved to be vvmdv, were used

    本研究通過三個方面進行了研究，取得了如下主要實驗結果： 1mdv不同致病型（ pathotypes ）毒株meq基因序列的比較研究應用pcr技術擴增了不同致病型mdvs ，即國際通用型疫苗毒cv1988 rispens株和中國特有的疫苗毒814株、特超強毒648a株（ vv + mdv ）以及6個廣西分離到的野毒株共9個毒株的meq基因，進行核苷酸序列的測定並與標準強毒ga株（ vmdv ）進行核苷酸和氨基酸序列的比較。
  15. Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected

    Rapd （隨機擴增多態性dna ）技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術，它建立於聚合酶鏈式反應（ pcr ）技術基礎之上，利用隨機合成的寡聚核苷酸序列為引物（一般為10個bp ） ，分別與dna的兩條單鏈結合，在dna聚合酶的作用下，對基因組的特定區域進行pcr擴增，其電泳結果為不同大小和數目的dna譜帶即rapd圖譜，可反映基因組相應區域的dna多態性。
  16. The proviral genome cdna clone of snv strain was digested, and the fragments was cloned into the puc18 vector, sequenced, finally we got the sequence of snv genome. the two pairs of primers were designed and synthesized according to the snv strain env sequence. the env genes of the rev isolates isolated from china were amplified by polymerase chain reaction ( pcr )

    根據網狀內皮增生癥病毒snv株env基因的序列，設計並且合成了兩對引物，利用該引物，以中國地方分離株sd9901 、 ha9901前病毒基因組cdna為模板，通過pcr技術，成功的從國內分離得兩株rev毒株中擴增出env基因，並將之克隆進pucm - t載體中測序。
  17. Three serial chest x - rays were all normal. polymerase chain reaction ( pcr ) tests and serology test results were positive for sars coronavirus on 8 september

    9月8日，患者的聚合?鏈反應測試及血清測試顯示對嚴重急性呼吸系統綜合癥的冠狀病毒呈陽性反應。
  18. Different kinds of biochemical reaction approaches for the goal of detection and kinetics studies on microfluidic chips are reviewed, including enzymatic assay, immunoassay, enzyme - linked immunosorbent assay ( elisa ), polymerase chain reaction ( pcr ) and nitric oxide release reaction

    摘要綜述了近幾年來在微流控晶元上以檢測或動力學研究為目的而進行的各種生物化學反應技術，包括酶反應、免疫反應、酶聯免疫反應、 pcr反應和含氮氧化物釋放反應。
  19. The detection of ribozyme gene with two cleavage sites that cleaves plrv replicase gene were present in the second part of this paper. a conserved sequence of 35s promoter of plrv was found in gene pools. two primers were designed based on the conserved sequence and bamh i and ribozyme gene. the genomic dna of potato was amplified by the primers through polymerase chain reaction ( pcr )

    從許多資料中報道的plrv的35s啟動子的序列中找出一段保守序列，然後根據與核酶緊密相連的bamh和這段保守序列設計兩段引物，用這兩段引物通過pcr擴增轉基因馬鈴薯的基因組dna ，並進行檢測。
  20. After plasmid and cherosome dna extraction and purification, the gyra of 16 isolates of salmonella gene was amplified by polymerase chain reaction ( pcr )

    測定了16株致病性沙門氏菌對12種抗菌藥物的最低抑菌濃度（ mic ） ，依照美國臨床實驗室標準委員會（ nccls ）標準（ 1990 ）判定菌株耐藥性。
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