positive fusion 中文意思是什麼
positive fusion
解釋
強化熔煉-
In the 1990s, the pheromones of gram - positive bacteria, which regulates the growth and toxin secretion of the same type bacteria, were identified they were peptides consisted by dozens of amino acids. the pheromones can auto - recognize membrane receptor of the identical types of bacteria. we had constructed a fusion protein named pheromonicin by introducing a staphylococcal pheromone agrd at the c - terminal of the colicin ia
丘小慶等利用信息素的自主導向特性和大腸菌素ia的致死性通道特性構建了由金黃色葡萄球菌信息素agrd和大腸菌素ia組成的融合蛋白，命名為pheromonicin ，該蛋白表現出了信息素和大腸菌素ia都不具有的抗金黃色葡萄球菌活性。 -
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
It can also be analyzed from non - linear way : 1, the knowledge construction is a process of positive internalizing ; 2, the knowledge construction is a process of actively producing ; 3, the knowledge construction is a process of integration ; 4, the knowledge construction is a process of positive externalizing ; all these shows that knowledge construction is not simple transplantation of exterior knowledge but the knowledge construction based on the original experience, mental state and beliefs, it is the fusion of various horizont. the knowledge construction is the process of internalizing and externalizing, producing and integration, the unity, of inheritance and creation
第四，也是各種知識的外化過程。可見，教師建構教育知識不是外部的公共教育知識及其結構向教師個人內部的簡單移植，而是以教師原有的經驗、心理結構為基礎的知識建構，是多種視界的融合。知識的建構是內化與外化、生成與整合的過程，是繼承與創新的統一。 -
The expression products were tested by wester - blotting, it demonstrated the fusion proteins can be recognized by positive serum of hypoderma sp
通過westem ? blotting分析表達產物，表明表達產物具有免疫活性，能被制備的陽性血清所識別。 -
Recombinated human ctla4ig fusion protein had been used experimentally and clinically in prevention and treatment of transplant rejection, autoimmune diseases and allergic diseases, and positive curiative results were obtained
重組人ctla4ig融合蛋白已被運用於防治移植排斥反應、自身免疫性疾病和變應性疾病等的實驗和臨床研究，並取得了肯定的效果。 -
This expression vector plbcas - hsa - lgl has the following advantages : i ) the 1. 7kb promoter is able to drive cell - specific and hormone - dependent expression ; ii ) the inclusion of intron - 1 can increase expression level of fusion genes ; iii ) the 5 ' utr of bovine p - casein mrna may have a positive role in both transcriptional and post - transcriptional regulation ; iv ) the gfp gene make the selection of positive clone among embryos possible ; v ) the gfp gene can be easily excised via cre - mediated recombination between the two loxp sites after the expression vector has been integrated into chromosome ; vi ) the two incompatible lox sites, loxp and lox2272, would facilitate cre - recombinase mediated cassette exchange ( rmce ), which in theory will leading to develop a technology of site - specific gene expression in animal mammary glands
該載體的特點是：具有可以調控外源基因在乳腺中特異表達的牛-酪蛋白基因5 `端側翼區和包括第一外顯子及內含子在內的5 `端調控區；將人血清白蛋白cdna準確地置於牛-酪蛋白基因第二外顯子中的翻譯起始密碼子atg之後，而且沒有增加額外的序列和使人血清白蛋白cdna移碼；引入標記基因gfp ，便於在胚胎期鑒定陽性胚胎，減少受體；引入cre lox重組系統： （ ? ）標記基因gfp的兩端的兩個loxp位點可以在表達載體整合到基因組后，刪除標記基因； （ ? ）餘下的一個loxp位點可以和前面的lox2272位點組成cre重組酶介導的盒式交換系統。 -
Hybridomas secreting monoclonal antibodies ( mcabs ) specific for clostridium perfringens type a enterotoxin ( cpe ) were produced by fusion of nso myelomas cells with spleen cells from balb / c mice immunized with purified cpe. wells containing hybridomas secreting immunoglobulin g ( igg ) antibodies against cpe were specifically identified by an indirect enzyme - linked immunosorbent assay ( elisa ), and two strains of elisa - positive hybridomas were selected and cloned forth by limited dilution
該純化cpe對小白鼠的半數致死量為2 . 5ug /只；該cpe豚鼠皮膚藍斑單位為2500 4000 ；引起紐西蘭白兔小腸積液的最小毒素量為25ug 。將純化的cpe ，在含0 . 4福爾馬林的的pb液中透析制備類毒素。 -
The cellular localization of hse was done by using in situ hybridization, the results showed that primary spermtatocytes and spermatids in seminiferous tubular have positive signals. the subcellular localizations of hsei and hseii were identified by gfp fusion protein. hsei - gfp fusion protein was evenly distributing in cytoplasm, while hseii - gfp was not evenly distributing in cytoplasm and there were many bright spots in cytosol
用gfp高合蛋白的技術確定hsei和hsell在細胞中勺定位，結果顯示hsei gfp均勻地分佈於細胞漿中，而hsell0fp則分佈不均勻，在胞漿有聚集成許多熒光信號很強的點。 -
The expressed products were purified by metal ( ni2 + ) chelation affinity chromatography and tested by western - blot. the results showed that his - tagged vp6 fusion proteins have a positive reaction with anti - jl94 serum
採用western - blot對表達產物及純化產物進行反應原性分析，結果表明所得his - taggedvp6融合蛋白具有較好的抗原反應特異性。 -
After the pbd i and pbd ii gene ligated with the expression vector pinpoint ? a - 3, the recombinated plasmid ppd - 1 and ppd - 2 was transformed into jm109 strains, 4 positive clones were screened by restriction enzyme analysis, dna sequencing showed that two out of 4 positive clones inserted sequence of the constructed plasmid, which was the same as that of pbd - i and pbd - ii gene respectively, and its reading frame was correct, thus its could be used to express fusion protein
將pbd - 、 pbd -基因與表達載體pinpoint ~ （ tm ） xa - 3連結后獲得的重組質粒ppd - 1 、 ppd - 2轉化于大腸桿菌jm109中。經抽提質粒、酶切分析及pcr擴增，分別篩選到4個陽性克隆，將其中二個陽性克隆由測序分析，證實1個含pbd i基因片斷， 1個含pbd 11基因片斷，且閱讀框正確，可用於融合蛋白表達。 -
Purification of the recombinant expressed endostatin in this work we have successfully cloned mouse endostatin gene, and constructed pbv220 - endostatin expression plasmid that is a non - fusion expression vector. the positive pbv220 - endostatin was transformed into dh5a, and expressed mouse endostatin protein successfully. this study is the basis of the endostatin gene - engineering production
本文成功克隆鼠內皮抑素基因，構建了內皮抑素基因的原核表達載體pbv220一endostatin ，該載體為非融合性表達載體，將pbv22o一endostatin成功轉化到大腸桿菌表達菌dh5a中，並誘導表達了鼠內皮抑素蛋白，為基因工程方法生產內皮抑素奠定一定基礎。 -
Arginine rich human histone h3 is a kind of basic nucleosome protein. in the medium of physiological condition, by electrostatic interaction, histone h3 which arginine impart to it a positive charge binds to dna which phosphate groups impart to it a negative charge. in this paper, in order to establish a new technology of transfection, the functional domains la and ii gene segments of pea were lined with histone h3 gene segment, then a recombinant fusion protein was constructed which serves as c arrier for transfer of dna via receptor - mediated endocytosis
本實驗在已成功獲得的pea功能區a ，功能區基因片段與人組蛋白h3基因片段的克隆菌株的基礎上，構建原核表達載體，將克隆質粒pmd - 18t - pea經nco ， mlu雙酶切，回收酶切產物；將克隆質粒pmd - 18t - h3經mlu ， xho雙酶切，回收酶切產物。
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