prokaryotic system 中文意思是什麼

prokaryotic system 解釋
原核系統
  • prokaryotic : 初核質
  • system : n 1 體系，系統；分類法；組織；設備，裝置。2 方式；方法；作業方法。3 制度；主義。4 次序，規律。5 ...
  1. Compared to the prokaryotic gene expressing systems and mammalian gene expressing systems, insect gene expressing systems possess the stronger ability of the transcription and post - translation processing, which also has high production. we can anticipate it will be a kind of potent effective ectogenesis eukaryotic gene expressing system

    與原核表達系統和哺乳動物細胞表達系統比較，昆蟲細胞表達系統既有較強轉錄和翻譯后加工修飾能力，又有高表達量等特點，可望成為基因產業中一種比較理想的外源真核基因表達系統。
  2. Human bone morphogenetic protein 3 is a member of tgf - b superfamily. lt can induce the differentiation of cartilage and bone tissue in mesenchymal cell. and is important to bone self - repairment and bone development during embryo morphogenesis. in addition, some other biological activities of hbmp - 3 have also been found. such as inducing development of embryo and stimulating differentiation of neural and blood cells. therefore, there is a great prospect in the use of hbmp - 3. there is trace content of hbmp - 3 in human body. it has been expressed in the expression system of eukaryotes and prokaryotes respectively, but its application is restricted because of defects in the process and modification after translation in prokaryotic cells and higher costs and lower yields existed in eukaryotic expression system

    人骨形成蛋白3 （ hbmp - 3 ）屬于tgf -超家族的一員，可以誘導間充質細胞分化為軟骨和骨，在胚胎時期骨骼發育和骨再生修復中起著重要的作用，而且對胚胎發育過程中中胚層的誘導和分化、造血組織的發育以及神經系統的發育和修復等都起著重要作用，因而hbmp - 3有廣闊的市場前景。它在人體內含量極微，盡管研究人員已經在原核細胞和真核細胞表達系統中分別進行了表達，但是由於原核表達系統缺乏翻譯后的加工修飾，真核表達系統存在成本高、產量低等特點，限制了其在臨床上的應用。
  3. One was cloning, sequence analysis and expression of the fragment containing the b and c antigenic sites locating at the 5 " terminus in spike gene of tgev in prokaryotic expression system ( fused with gst ), the other was preparation of non - radioactive probe labeled by digoxigenin for detecting the rna extracted from tgev by assay of dot - blot

    為了鑒別診斷tgev與prcv及對tgev進行流行病學調查，本研究採用原核表達系統（ gst融合表達系統）表達tgev纖突蛋白（ s蛋白）中含有b和c抗原位點的多肽，並且制備了非放射性地高辛標記的核酸探針，通過斑點雜交（ dot - blot ）檢測tgev核酸rna 。
  4. Construction and filtration of the prokaryotic expression system of halr

    人肝再生增強子原核表達系統的構建與篩選
  5. Although the thymosin has been successfully expressed in the prokaryotic cell system, its application is restricted because of its deficiency of modification after translation

    盡管研究者已在原核細胞表達系統對胸腺肽的表達進行了研究，但由於原核表達系統缺乏翻譯后的加工修飾等缺點，限制了其應用。
  6. Western - blotting result demostrated rhpf4 had specific reaction with rabbit anti - hpf4 antibody. our system improve the expression level of r hpf4 by 80 fold compared with pt7 - 7 - r hpf4. after purified and renatured, r hpf4 prepared by our methods has bioactivity like wide hpf4. our study establish a stable base for further reseach of the h pf4 and provide a theoretics gist for modulative mechanism of eukaryotic protein expression in prokaryotic cells

    我們構建的rhpn原核高效表達系統經m page及凝膠密度掃描分析結果表明， rhpf4表達量占菌體總蛋白量的25 30 ，較原表達克隆pt7 7 rhpf4提高了近80倍，經快速高效的包涵體分高純化工藝和復性工藝， rhpf4具有野生蛋白活性。
  7. To prepare the wild type mbl in prokaryotic system, a pair of primers was designed and synthesized, and was used to amplify mbl gene from the recombinant vector pgem - mbl that contans wild type mbl cdna. a recombinant prokaryotic expression vector, pet28 - mbl, was constructed by inserted the mbl gene into plasmid pet28 ( b ), and after transfected it into ecoli bl21 ( de3 ) and induced with iptg, recombinant mbl protein was expressed successfully

    本實驗另選用了原核表達質粒pet28 （ b ） ，根據已構建好的含有mbl野生型基因的t載體pgem - mbl ，設計一對引物， pcr擴增mbl基因，凝膠回收，雙酶切pcr產物和pet28 （ b ）質粒， t4連接酶連接，轉化大腸桿菌dhsa ，氨芋選擇培養挑取克隆鑒定。
  8. It has not only many advantages of the prokaryotic expression system such as growing quickly, manipulating easily, strong power to express and control foreign protein, but also the ability to make foreign protein modified in post - translation. so pichia pastoris gene expression system is more appropriate to express many eukoryotic proteins than prokaryotic system and has attracted more and more people " s attention on it

    它不僅具有原核表達系統生長快速，操作簡單，外源基因表達量大並受到嚴格調控等特點，還具有真核生物特有的蛋白質翻譯后修飾的功能，表達真核生物蛋白質具有原核表達系統無法比擬的優越性。
  9. Several amino acid residues of sed have been proved to be important in the interaction between sed and mhc, but the mode of sed binding to mhc is not yet clear. this study is designed to explore the above issues. the main content and results of this study are as follows : firstly, the prokaryotic expression system of sed was constructed

    基於以上問題，我們對sed可能的活性位點進行預測，構建了一系列超抗原sed免疫識別機制的研究sed定點突變體，檢測這些突變體的mhc11結合活性和tcrvfi特異性，尋找與tcrv6結合的關鍵位點，進一步探討sed與mhc和tcr的相互作用方式。
  10. With the development of dna recombination technology, there are also some reports about the expression of recombined ifn protein by prokaryotic expression system, but the expressed proteins often consist in the form of inclusion body = the operations about extracting and purifying proteins are very complicated, biological activity of proteins are not high

    隨著dna重組技術的發展，國內外亦有利用原核表達系統表達重組ifn蛋白的報道，但表達的目的蛋白常以包涵體形式存在，提取純化操作步驟復雜、蛋白活性不高。
  11. Because of its special function, no successful example evolving the expression of antibacterial peptide directly in the prokaryotic system havs bben reported until now

    由於抗菌肽特殊的功能特點，所以至今還沒有能夠在原核系統胞內直接成功表達的範例。
  12. Establishment of prokaryotic expression system of cub7 - 8 fragment of hcubilin

    蛋白功能片段原核表達系統的建立
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