pronucleus 中文意思是什麼
pronucleus
解釋
n. 名詞 （pl. -clei ） 【動物；動物學】原核。adj. 形容詞 -clear
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While cortical alveoli discharge in batches, the plasma membrane has been reorganized time after time. spermaster " elongate and traction, and cytoplasm " flowage make the male pronucleus and the female pronucleus conjugate
）十物』 i ：他地仰排原核紀合是精人己光擴悵、引和細w質流動兒d 。 -
On the other hand, - 6 fatty acid desaturase injection assay will be performed to check whether a metabolic pathway is established. methords of research three plasmids vector with expression elements are used to establish a eucaryotic expression vector by restriction enzyme cutting and ligation. this vector is used to pronucleus microinjection
本實驗以pegfp - n1質粒為骨架載體，用酶切連接的方法構建一個順序含有- actin啟動子、 fad2cdna 、 sv40polya加尾信號的真核表達載體，雙切線性化后回收，使用回收的表達載體經原核顯微注射生產轉基因小鼠。 -
Using the hprt gene as a positive control, our result suggested that both the testis tissue and the male embryos from which sry transcription can be detected failed to yield any positive results of xist. female embryos at the pronucleus stage and 2 - cell failed to produce any positive result of sry and xist too. while since the 4 - cells period, xist is constantly transcribed until blastocyst stages
然後利用實驗一確定的pcr條件，以hprt為陽性對照，用巢式rt - pcr對小鼠早期胚胎進行xist基因的轉錄分析，結果發現，轉錄sry基因的睪丸組織以及雄性胚胎，從受精卵發育到囊胚的過程中，基本上不轉錄xist基因；不轉錄sry基因的雌性卵母細胞和雌性胚胎，從出現原核開始，到發育至2 -細胞期的過程中， xist基因一直不轉錄，但是，從4 -細胞期開始，一直到孵化前囊胚階段，雌性胚胎都轉錄xist基因。 -
The percentage of oocytes cleaved and formed pronucleus in either 0. 625 ( ag / ml or 1. 25ug / ml chx group was significantly higher than the control group ( p < 0. 05 ). however, when the concentration of chx was increased to 5 ug / ml, the cleavage rate and pronuclear formation rate were decreased significantly ( p < 0. 01 )
結果培養在0 . 625和1 . 25 g mlchx的卵母細胞的激活分裂率和總原核形成率明顯高於對照組（ p 0 . 05 ） ，而培養在5 g mlchx的卵母細胞的激活分裂率和原核形成率則極顯著低於對照組（ p 0 . 01 ） 。
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