proviral 中文意思是什麼
proviral
解釋
原簿的,前簿的-
The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry. the su of retroviruses is a highly variable genetic element, containing receptor binding sites and major antigenic determinants. exjsrv - specific dna probes were derived. by using these dna probes in tissue hybridization. we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals, validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛隨機引物法標記exjsrv特異的env片段，制備探針，原位雜交檢測spa肺組織中的rna及前病毒dna ，結果表明spa患羊肺組織內有jsrvenv基因mrna的表達，同時也檢測到了前病毒dna ，而相應的陰性對照卻無陽性信號，證實外源性病毒特異的dna探針在致瘤性前病毒的整合位點和整合的外源性前病毒的檢測中具有可信度。 -
The proviral genome cdna clone of snv strain was digested, and the fragments was cloned into the puc18 vector, sequenced, finally we got the sequence of snv genome. the two pairs of primers were designed and synthesized according to the snv strain env sequence. the env genes of the rev isolates isolated from china were amplified by polymerase chain reaction ( pcr )
根據網狀內皮增生癥病毒snv株env基因的序列，設計並且合成了兩對引物，利用該引物，以中國地方分離株sd9901 、 ha9901前病毒基因組cdna為模板，通過pcr技術，成功的從國內分離得兩株rev毒株中擴增出env基因，並將之克隆進pucm - t載體中測序。
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