purification by chromatography 中文意思是什麼

purification by chromatography 解釋
色譜提純
  • purification : n. 1. 清洗，洗凈；凈化（作用）。2. 提純，精製。3. 【宗教】滌罪；潔身；齋戒；潔禮，祓；【天主教】潔杯式。
  • by : adv 1 在側，在旁，在附近。2 （擱）在一邊，（放）到旁邊，（存）在一旁；收著。3 （由旁邊）經過，過...
  • chromatography : n. 【化學】層析，色層（分離）法。
  1. 6 - phosphogluconate dehydrogenase ( 6 - pgadase, ec 1. 1. 1. 44 ) was isolated by homogenate, ammunium sulfate fractionation, deae - sepharose chromatography, blue - sepharose affinity chromatography and gel filtration with sephadex g - 200 from bacillus subtilis, and some properties of the enzyme had been studied. a 113. 8 - fold purification was obtained with a 8. 2 % yield. the purified enzyme moved as a single electrophoretic band in page

    將枯草芽孢桿菌超聲波破壁后的粗提取物進行分段鹽析、 deae - sepharose陰離子交換柱層析， blue - sepharosecl - 6b特異結合柱層析和sephadexg - 200凝膠過濾等純化步驟，得到聚丙烯酰胺凝膠電泳為單一蛋白區帶，比活為1 . 46u mg的酶制劑。
  2. Purification of isomalto - oligosaccharide by cation exchange chromatography

    離子色譜分離法提純異麥芽低聚糖
  3. Purification and characterization of phytase from a. niger an 01001 a. niger an01001 was inoculated on solid media and cultivated at 30 for 5 days. proteins were extracted from solid - state fermentation with 50mm acetate buffer ( ph5. 5 ). the molecule weight of the phytase protein was determined as about 78kd by sds - page. the purification procedures include ammonium sulfate precipitation, deae - cellulose ion - exchange chromatography, gel electrophoresis and electroelution

    3 .植酸酶的分離純化及其性質研究黑麴黴ano1001經固體發酵，用緩沖液抽提后，經硫酸按沉澱， deae一纖維素離子交換層析，聚丙烯酞胺凝膠電泳和電洗脫等純化步驟獲得的植酸酶，用sds一page檢測為一條均一譜帶，其分子量約為78kd 。
  4. Isolation and purification of biliproteins by chromatography on anion ion exchange and hydroxylapatite

    陰離子交換和羥基磷灰石色譜從螺旋藻中提純藻膽蛋白的研究
  5. At the same time an apparatus of zone melting is designed, the method of zone melting is used to purify the purified product of recrystalizing. after purification, the homogeneity and stability of the purified product of antioxidant d and bht are examined by high performance liquid chromatography ( hplc )

    實驗研究結果：防老劑d重結晶提純工藝為：每次取10g工業品防老劑d ，經過堿洗后，去離子水洗滌至中性，溶於100ml乙醇中，同時用粉狀活性炭（ 1g次）脫色、過濾、母液靜置2h后，再放入冰箱結晶2h 。
  6. Study on purification of rhepo by the immunoaffinity chromatography

    免疫親和層析法純化重組人促紅細胞生成素的實驗研究
  7. The isolation and purification of dnaase in the earthworm the earthworm dnaase was purified from the tissue extract of earthworm by denaturing the protein with low ph buffer, high temperature, ammonium sulfate precipitation, deae - cellulose ( de52 ) chromatography and ultra - filter membrane

    雙胸蚓組織中dna酶的分離純化雙胸蚓組織粗提取液經過選擇性酸變性、選擇性熱變性、硫酸按分段鹽析、 deae一纖維素（ de52 ）柱層析、超濾膜分級分離后得到一個電泳純的dna酶。
  8. An active metabolite was obtained by purification with precipitated by ethanol, sephadex g - 25 gel, deae - cellulose ion exchange resin and silica gel column chromatography

    經乙醇（ 95 ）沉澱、 sephadexg - 25凝膠層析、 deae -纖維素離子交換層析和硅膠層析純化，得到抑制黑麴黴生長的單一組分。
  9. The purification of this halocin was achieved by combination of tangential flow filtration ( tff ), sephadex g50 and deae - sepharose chromatography. the n - terminal amino acid sequence was also determined by edman degradation

    Halc8對大多數的極端嗜鹽古菌（包括部分嗜鹽堿古菌）有抑制作用，但對細菌則無明顯的抑制作用。
  10. Bioassay showed that the second - stage juvenile ( j2 ) could be killed by the raw extract of serine protease. serine protease was characterized after purification with ion exchange chromatography and gel filtration chromatography. the t profile, t stability profile, ph profile, substrate specificity and inhibitor were tested

    該酶分子n -端的氨基酸序列為avidtgveashpef ，通過n -端氨基酸序列設計簡並引物，提取被毛孢owvt - 1的總rna ， rt - pcr克隆了該酶的成熟肽編碼基因（ 1000bp ） ，序列分析表明，絲氨酸含量高達12以上。
  11. Nuclear energy - chemical separation and purification of uranium and plutonium in nitric acid solutions for isotopic and dilution analysis by solvent chromatography

    核能.用溶劑色譜法對同位素和稀釋度分析用的硝酸溶液中鈾和鈈的化學分離和提純
  12. Purification and renaturation of recombinant human cu, zn - sod by metal - chelating affinity chromatography

    金屬螯合親和層析純化和復性重組人銅鋅超氧化物歧化酶
  13. 3. successful purification of the crude ha was achieved by anion exchange resin deae - cellulose column chromatography using 0. 6mol / lnacl solution as an eluant

    3 .採用陰離子樹脂deae一纖維素對透明質酸粗品進行純化，當naci為0 . 6mol / l時，蛋白質含量較低。
  14. Separation and purification of coenzyme q10 from microbial fermentation by high - speed counter - current chromatography

    10的高速逆流色譜法分離純化
  15. In this study, a novel strategy to inhibit the activation of the complement system has been developed. to get the purified clq as the target molecule for biopanning, the first step of our purification was to isolate the clq from clr2cls2 by affinity chromatography on igg - sepharose 4b column

    本研究另闢蹊徑，以補體經典激活途徑始動分子c1q為靶標，採用噬菌體肽庫技術，親和篩選能與c1q結合的噬菌體克隆，研製可抑制補體激活的c1q模擬短肽。
  16. After the gst fusion proteins were processed by factor xa and were purified by gluta - thione - sepharose 4b affinity chromatography, the recombinant protein hbrp with high purification would be acquired. during the process of separation and purification, the every result which can show the location of the aim protein was monitored by 10 % sds - page. the gst fusion proteins acquired finally were examined and analyzed by the methods of western - blottin

    原核表達系統具有表達效率高，操作方便等特點，但缺乏翻譯? 3 ?后修飾，以pgex載體表達的gst hbrp融合蛋白，具有表達的蛋白穩定性好，在體外易於純化等優點，表達的gst融合蛋白可用gluthioneseph刪sc4b進一步純化一factorxa酶切與測分離，從而大量獲得hbrp重組蛋白，而且一次回收率大約在90以上。
  17. Isolation and purification of total saponins from cornus officinalis by column chromatography of macroreticular resin

    大孔吸附樹脂法分離純化山茱萸總皂苷
  18. In this optimal condition, p450nor was expressed massively. the expressed product was then purified by deae - cellulose chromatography. the purified expressed protein showed one band in sds - page and the purification attained anticipative purpose

    在此條件下，大規模誘導表達重組細胞色素p450nor ，經deae纖維素色譜柱純化， sds - page分析表明，純化的目的蛋白基本上為單一譜帶，純化達到預期效果。
  19. The yeast recombinant expression vector pplc3. 5k - ndrg2 was constructed, then the ndrg2 protein was expressed in soluable form in pichia. after purification by metal chelate affinity chromatography, we get 6his - ndrg2 fusion which be nature in structure and will be useful to reseach its functions in future

    為研究ndrgz的結構與功能，構建了ndrgz的酵母融合表達質粒，並在比赤酵母中得到可溶形式的表達；利用金屬螫合親合層析純化出了6his融合的ndrgz蛋白。
  20. After dissolving inclusion bodies, renaturing inclusion bodies and further purification by immobilized metal ion affinity chromatography ( imac ), the fusion protein trx - scfv of electrophoretic purity was obtained, they still possessed antigen - binding activity

    經變性復性，用固相金屬離子親和色譜法一步分離純化了具有功能的單鏈抗體，在一升的發酵液中得到78mg的重組蛋白。
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