purification method 中文意思是什麼

purification method 解釋
凈化法
  • purification : n. 1. 清洗,洗凈;凈化(作用)。2. 提純,精製。3. 【宗教】滌罪;潔身;齋戒;潔禮,祓;【天主教】潔杯式。
  • method : n 1 方法,方式;順序。2 (思想、言談上的)條理,規律,秩序。3 【生物學】分類法。4 〈M 〉【戲劇】...
  1. The respiratory intensity of the contaminated soil decreased by 29. 93 % while ammonification and nitrification increased significantly than that of control soil. 2. extraction and purification of soil microbial total dna a method of extracting soil total dna was developed, and it can extract dna from g + bacteria

    二、土壤微生物總dna的提取和純化方法研究為了採用不依賴于培養的16srdna分析的方法研究有機磷農藥長期污染對土壤微生物群落結構的影響,建立了從土壤中提取總dna的方法,並通過改進使適合於對革蘭氏陽性菌的提取。
  2. This method is to obtain multi - fungi from water and waster water by methylene chloride fluid - fluid extract nitrogen, and then take the concentration purification to the highly effective liquid phase color spectrometer where it can be detected by the diode matrix detector

    摘要用二氯甲烷液液萃取氮吹濃縮法萃取水和廢水中的多菌靈,取濃縮純化后的有機相直接進樣到高效液相色譜儀,用二極體矩陣檢測器檢測,根據保留時間外標法定量。
  3. Some main results of testing research were as fellows : i. in the course of testing of leaching zinc dross, leaching zinc dross by sulfuric acid was researched and the effects on the leaching rate of the zinc dross were analyzed by author, the optimum conditions of leaching testing of zinc dross were determined as fellows : liquid - solid ratio 6 : 1, stirring ratio 250r / min, granularity 120 mesh, the soaking time 5h, the concentration of sulfuric acid of 30 %, and under these conditions, the rate of leaching zinc is above 92. 0 %. ii. in the course of testing of purification, on the basis of comprehending and comparing all present methods of the manganese - removal and iron - removal, the oxidizing method of manganese - removal in zinc sulfate solution using ammonium persulfate was put forward and the oxidizing method of iron - removal in zinc sulfate solution using the goethite process and oxidation - hydrolysis process was adopted

    研究的主要結果具體如下: 1 、研究了硫酸浸出鋅浮渣並分析了影響浸出率的各種因素,確定了浸出鋅浮渣的最佳條件為:液固比為6 : 1 ,攪拌速度為250r min ,粒度為- 120目,酸浸時間為5 . 0h ,硫酸濃度30 ,鋅浮渣的浸出率大於92 . 0 ; 2 、在綜合和比較了目前除錳和除鐵的各種方法基礎上,提出了過硫酸銨氧化除錳方法和選擇了針鐵礦-氧化水解聯合法除鐵方法,並分析和討論了影響除錳率和除鐵率等各種因素,確定了除錳的條件為:溫度90 , ph值5 . 4 ,反應時間3h ,過硫酸銨達到理論值的1 . 2倍,除錳率超過99 . 86 ;聯合法除鐵的條件為:氧化反應時間1 . 5小時、溫度控制在90 92 、 ph值5 . 2 5
  4. Several method for identification, separation and purification of hemocytes in lepidoptera

    鱗翅目昆蟲血細胞鑒定與分離純化的幾種方法
  5. The results shows that the vitro expressed protein of n gene by recombinant plasmid vector in the e. coli maintains anigenicity of tgev the recombinant protein was purified acconiing to the vector self characteristic ( hisk a polyhishdine tag introduced at the amino - acid terminus of the nucleoprotein allowed for the purification of protein by nickel - chelate dsity chromataography we explored all possibilities of pedcation and gained the modified purification method. several conditions, which include diffend ph buffer and concelltheion of imidazole, were selected to purify recombinan nucleorotein

    根據載體pproexhtb含有( his ) 6特點,將融合蛋白進行純化,在純化過程中經各項條件的探索,確定為在裂解液中含有1mmpmsf的條件下,分別經過2倍體積的buffera和bufferb洗脫后,再收集ph5 . 9 ,含有80mmol / l咪唑的1倍體積bufferc洗脫液,可得到純化的融合蛋白。
  6. Dig labeled probe hybridization with solid pcr product was performed as well as electrophoresis of liquid product, this method combines rna purification, pcr amplification and nucleotide probe hybridization detection together and has many advantages including better rna purity, less time consumption, reliable positive reaction and 10 times sensitivity as rt - pcr gel running detection, reduce false positive result, unpurified nucleotide requirement, loug infected plant organism can be detected by solid hybridization

    2 )結果可靠,雜交特異性誘捕目的片段,同時去除了核酸中存在的pcr抑制物質,減少了因核酸提取不純造成的漏檢現象,結果輸出通過雙重判定保證了結果的可靠性。 3 )靈敏度高,通過雜交進行結果判定,靈敏度比傳統的rt干cr大約高10倍。
  7. Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry

    三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞,離心,上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體,變性后,加人用pbs平衡過的以utal腸onesepb抓脫4b ,室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ,決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積,用預冷的1xpbs清洗cldtathionese戶, se4b ,得到50 %的基質
  8. In order to study the function of cycling2 in vitro culturing cell line, we used pires - g2 eukaryotic expression vector transfecting human gastric cell line sgc - 7901 and human embryo kidney hek - 293 cells by lipofectamine plus reagent, and studied the function of cycling2 expression on the cell proliferation in vitro, further investigated the regulation mechanism of cycling2. at the same time, we made a study on the expression level change of cycling2 in normal gastric tissue and different type and different stage of gastric carcinoma tissue. material and method 1 material : piresneo vector was purchased from clonetech, plasmid extraction and purification kit was purchased from qiagen company ; rpmi 1640, dmem fetal calf serum were obtained from gibco / brl ; lipofectamine plus and g418 were purchased from life technologies ; ultrasensitive ? s - p kit, mouse monoclonal antibody p21wafl ( in use ), dab staining kit were purchased from maixin company

    實驗材料與方法1 .實驗試劑高糖dmem 、 rpmll640和胎牛血清購自美國g山eo / brl公司; dmewf12 ( 1 : 1 )混合培養液購自美國hyclone公司;胰蛋白酶購自美國si目叮a公司; hepes由美國amersco公司分裝;脂質體轉染試劑( upofectalnineplusreageni )和以18為美國玩vitrogen公司產品; piresneo載體購自美國cloneteeh公司;質粒提取及純化試劑盒購自德國qiagen公司; ultresensitive翎s一p免疫組織化學試劑盒;鼠單克隆抗體戶3 ( do一7 )蛋白(即用型) ;鼠單克隆抗體p21waf , (即用型) ; dab染色試劑盒均購自福建邁新公司;鼠單克隆抗體pziwa曰(濃縮型) ;辣根過氧化酶標記羊抗鼠二抗購自北京中山公司; ecl試劑盒購自美國santacruze公司; dcproteinassay試劑盒購自bi 。
  9. At the same time an apparatus of zone melting is designed, the method of zone melting is used to purify the purified product of recrystalizing. after purification, the homogeneity and stability of the purified product of antioxidant d and bht are examined by high performance liquid chromatography ( hplc )

    實驗研究結果:防老劑d重結晶提純工藝為:每次取10g工業品防老劑d ,經過堿洗后,去離子水洗滌至中性,溶於100ml乙醇中,同時用粉狀活性炭( 1g次)脫色、過濾、母液靜置2h后,再放入冰箱結晶2h 。
  10. We found it a key to pay attention to the facts of current efficiency as well as relative ferrate concentration and simplifying the procedure of precipitation and purification so as to raise the technique level of preparing ferrate ( vi ) by the method of electrolysis

    本文通過總結現有制備高鐵酸鹽的工藝方法尤其是電解法的有關文獻發現,兼顧電流效率和相應的高鐵酸鹽濃度水平以及簡化結晶純化手續是提高電解法制備高鐵酸鹽工藝水平的關鍵。
  11. This dissertation mainly focuses on studying these three parts. 1. two types of dna purification chips based on the method of spe ( solid phase extraction ) have been fabricated and studied

    其主要技術是製作微流路的生物晶元( microfluidicbiochip ) ,針對這些,本文主要的研究內容如下: 1
  12. This method of isolation, cultivation and purification of the synovial cells in vitro is proved to be simple and feasible

    結論本研究建立了簡單易行的滑膜細胞分離、培養和純化方法。
  13. The main contents and results of the study are as follows : 1. the separation, culture and purification of black bear fibroblast cell for the donor of nuclear transfer the black bear fibroblast cell can be obtained from ear skin and neck skin of the black bear by issue culture method. the cells were cultured in dmem and rpmi1640 mediums containing 10 % fetal bovine serum ( fbs ), respectively

    主要內容和結果如下: (一)黑熊成纖維細胞的分離培養和純化從黑熊耳緣皮膚和頸部皮膚取材,採用組織塊培養法分離培養黑熊皮膚成纖維細胞,在dmem和rpmi1640兩種培養基中分別添加10的胎牛血清,均可滿足黑熊皮膚成纖維細胞的體外生長和傳代。
  14. This new method plays an important role in the field of basic biological research, medical diagnosis and clinical therapy. because of its high - sensitive and specific interaction between macromolecules, spr biosensor is used for directly examining the reaction between targeted protein and corresponding antibody without careful purification

    Spr生物傳感器的高靈敏性,以及生物大j皮巴」 a碩士芹仁論文v沉孟y吁」匹r 』 sn正「分子間反應的高度特異性,使不需要嚴格純化而直接檢測目的蛋白與抗體間的反應成為可能。
  15. During the course of purification, the method of orthophenanthroline staining is used to detect the ferritin. 30 | il of the fractions eluted from the aca ^ gel column were pulled into the cells of the minim incubation plate. then sopil 20 % dithionite and 40 | il 0. 1 % orthophenanthroline solution were pulled into respectively

    在酶標板小孔中加入30 l待測液,加入30 l20的連二亞硫酸鈉溶液還原后,再加入40 l0 . 1鄰菲咯啉溶液,根據有無桔紅色顏色產生確定是否含鐵蛋白。
  16. A treatment method of super - saturation steam for deposited salt in steam turbine is introduced, output of the steam turbine is therefore improved through purge. purification process and attentions are introduced

    摘要介紹了背壓式汽輪機積鹽積垢的處理方法?過飽和蒸汽法,通過清洗使汽輪機的出力率得以提高。介紹了清洗的過程和注意事項。
  17. Study on the rapid purification of rat islet cells with push - type tube by modified mono - density gradient method

    推進式離心管結合單密度梯度法快速純化大鼠胰島
  18. 3 a new purification method of cnts was present. the cnts films were prepared with the cnts purified by the former method, and the structures and the properties of the films were measured

    提出一種新的碳納米管( cnts )的純化方法以及把用此方法純化的cnts制備成膜的過程及性質的初步探索。
  19. Compared with other methods, aquatic plant purification method shows unique feature

    通過與其他方法的比較,說明了水生植物凈化法有其獨特的優點。
  20. We have developed an easily purification method, by changing the polarity of solvent, to purified cds nanoparticles with narrow size distribution

    並利用溶濟極性的不同,可分離不同大小尺寸分佈的奈米微粒,進而取得更均勻分佈的硫化鎘奈米微粒。
分享友人