rabbit igg 中文意思是什麼
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Test 3 : detected activity of serum and immunoglobulin samples by indirect elisa test, rabbit antibody against foxes ' igg and hen ' s labeled with hrp igy was used in indirect elisa test
試驗三：間接elisa檢測的抗體活性。用過氧化物酶標記的兔抗狐igg和雞igy抗體進行間接elisa測定獲得的樣品的活性。 -
Purified chicken igg was used to immune rabbits, then the rabbit antichicken igg was purified and the rabbit antichicken igg conjugated with hrp was made
以純化的雞igg免疫兔，制備辣根過氧化物酶標記的兔抗雞igg 。 -
Anti - p21 mouse monoclonal antibody from beijing zhongshan biotechnology anti - mouse or anti - rabbit igg secondary antibody from santa cruz biotechnology ly294002 from sigma biotechnology tritonx - 100 from boehringer mannhein gmbh fluorescein isothiocyanate ( fitc ) conjugated anti - mouse igg antibody was purchased from beijing zhongshan biotechnology hepes from e. metck darmstadt methods superovulation and collection of eggs for superovulation, female kunming mice 4 - 5 week old were injected with pregnant mare serum gonadotropin ( pmsg ), and after 46 - 48 hours with human chorionic ginadotropin. ( hcg ). one - cell fertilized eggs were collected on the next day from oviduct of females
取4一5周齡成熟雌性昆明系小白鼠，腹腔注射pmsg （孕馬血清促性腺激素） 10iu ， 46一48小時后腹腔注射hcg （人絨毛膜促性腺激素） 1oiu ，將注射hcg后的雌鼠與8周以上的成熟雄鼠合籠交配，次日檢察陰栓，將查到陰栓的雌鼠處死，取輸卵管于mz培養液中，解剖鏡下撕開壺腹，釋放細胞團，然後用300林歲nil透明質酸酶消化去除顆粒細胞，口控吸管將卵細胞在m :中反復清洗，然後置於孵箱中，根據時間點收集g2期細胞。 -
The optimum conditions of dot - elisa was determined : the optimal coating concentration was 8. 9 u g / ml, 1 : 400 was the best working concentration of hrp - laleled rabbit antichicken igg, the other steps and reagentes were also optimized
抗原最適包被濃度為8 . 9 g / ml ，酶標抗體最適工作濃度為1 ： 400 ，確定了其他反應試劑和各步的最佳反應時間。 -
The components of 37kd and 39kd glycoproteins from gel were immunized respectively mice and polyclonal antiserums were obtained. glycoproteins of sperm cell surface can be examined using igg rabbit anti mouse - colloidal gold as probe
用它們分別免疫小鼠得到多克隆抗體，以兔抗鼠igg膠體金15nm為探針，檢查此兩種蛋白在精細胞質膜上的分佈。
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