random pcr 中文意思是什麼
random pcr
解釋
隨機聚合酶鏈反應-
Those easily found plants and fungi are pinus densiflora, querces mongolica, lespebeza bicolor, rhododendrum chrysanthum, melampyrum roseum, ramaria stricta, cortinarius collinitus, etc, among which, 22 species of plants and 3 species of mushrooms were first reported occurring beside fairy rings of matsutake. 4. studies on dna polymorphisms of symbiotic edible fungi, tricholoma matsutake. the dna fingerprinting of wild baidiocarps of matsutake, collected from the scenes of major production regions in china, were analyzed based on rapd ( random amplified polymorphic dna ) - pcr patterns were optimized by example experiments of two dna templates in this study
除了常見的赤松（屍inusde胭必ra ） 、蒙古棟（ que兀esm口吧口lica ） 、胡枝子（眾印『 bezabicolor ） 、興安杜鵑（ rhododen動側nc加」 anthum ）等外，另外以前並未報道的22種植物， 3種高等真菌也在松茸蘑菇圈附近出現頻率很高，例如被當地菇農稱為松茸花的山蘿花（ mela州眨」似腳roseum ） ，與松茸同期發生的蘑菇如密叢枝（ ramariastricta ） 、粘柄絲膜菌（ cortinari 。 -
The materials as explant in transformation come from birch leaf, stem segment and leaf stalk, and the spider toxin gene was used as foreign gene for this transformation experiment. it showed that the best explant was the big leaf, on which the transformation frequency was 22 %. by gus detection, there were 43 percent of the plants with kanamycin resistance, and 100 percent of positive result, by pcr amplification, was gotten from random sampling
利用雙元載體的根癌農桿菌lba4404菌株（ agrobacteriumtumefaciens ） ，含質粒pyhy （目的基因及npt 、 gus基因） ，對白樺試管苗莖段，葉柄，葉片三種外植體進行侵染，結果表明：大葉片生長勢強，為轉基因的最優外植體，轉化率能夠達到22 。 -
In this work, some species of palmae cultivated in the xiamen botanical garden had been selected to be analyzed their genetic diversity with rapd ( random amplified polymorphic dna ) technique. according to the result of genomic dna amplified with pcr ( polymerase chain reaction ), genetic distance and similarity between different palm species were calculated on nei " s estimate of similarity and genetic distance. a primary but first time research at the phylogenetic relationships of some genera and species, the molecular classification and identification of some puzzling species of palmae was carried out through upgma ( unweighted pair group mean average ) cluster analysis of the genetic distance together with comparative study of the morphological structure characteristics
本文在初步調查分析了棕櫚科植物在我國的自然分佈、引種馴化情況以及該類植物在廈門地區栽培應用狀況的基礎上，首次採用rapd分子標記技術，對廈門萬石植物園引種的一些棕櫚科植物的遺傳多樣性進行了研究，根據pcr對基因組dna擴增的結果，用nei ' s相似性系數計算了不同植物間的遺傳距離和遺傳一致度，通過對遺傳距離的upgma聚類分析，並結合形態分類的特點，對棕櫚科植物的屬、種間的系統分類關系和一些疑難種的分類鑒定進行了初步研究。 -
Dominant negative alleles of gpil7p were produced by random pcr mutagenesis of three conserved fragments of gpi17p : 1 ) having generated the plasmid library which contains 100000 plasmids harboring gpi17 mutants then screened from 8000 clones, we got 25 strong and 3 very strong dominant negative alleles
對gpi17p可能的三個保守區進行了pcr隨機誘變，並最終獲得顯性負性等位基因： 1 ）組建了gpi17p突變體質粒庫，其所含質粒數約為100000 。 -
By sun, bo ( major : marine biology ) directed by professor zhang, guofan and dr. liu, xiao population genetics of four argopecten irradians cultured populations was analyzed by using random amplification polymorphism dna ( rapd ) techniques. and partial dna sequences of the internal transcribed spacers ( its ) from four representative samples ( one sample represent for each population ) were amplified by pcr and sequenced in order to stress the phylogenetic relationships of the four populations. besides, by rapd technique, two argopecten irradians families were studied to examine whether the hybridization experiments were successful and which family was more suitable to be materials in future genetic linkage map construction
本文以海灣扇貝4個養殖群體為研究對象，採用隨機引物多態性dna （ rapd ）技術進行了群體遺傳學研究，並嘗試使用4個群體中代表個體的內轉錄間隔區（ its ）序列比對分析結果作為參考；另外，本文還運用rapd技術對人工異體交配的2個海灣扇貝家系的雜交成功率進行了分析，均獲得成功證實，並評估了兩個家系構建遺傳連鎖圖譜的潛力。 -
Rapd ( random amplified polymorphic dna ), which bases on the polymerase chain reaction ( pcr ), is by far one of the most commonly molecular techniques to uncover dna sequence polymorphisms. the basic priciple of this technique is that an arbitrary primer ( usually lobp oligonudetide ) is used to amplify random segments of dna, and a small number of fragments will be amplified when the primer anneals on each strand over a length range. if sequence variation is present at the priming site, then a fragment may not be amplied, so the dna polymorphic can be detected
Rapd （隨機擴增多態性dna ）技術是二十世紀90年代發展起來的一項dna分子多態性檢測技術，它建立於聚合酶鏈式反應（ pcr ）技術基礎之上，利用隨機合成的寡聚核苷酸序列為引物（一般為10個bp ） ，分別與dna的兩條單鏈結合，在dna聚合酶的作用下，對基因組的特定區域進行pcr擴增，其電泳結果為不同大小和數目的dna譜帶即rapd圖譜，可反映基因組相應區域的dna多態性。 -
Finally, the variant line tc243 - 2, was analyzed by rapd - pcr amplification with 100 random primers. the results suggested that phenotypic variation came from exogenous dna transformation rather than exogenous pollen contamination. a mutation corre
用100條隨機引物對tc243一株系的rapd一pcr擴增結果表明該株系的變異不是來自外源花粉的污染，同時發現了特異條帶5270 _ 1600對應的變異在tz一ts代能連續穩定遺傳。 -
In this experiment, the cultured virus was detected by pcr amplification. moreover, 3 natural sheep cases, 16 sheep cases and 16 goat cases at random were diagnosed. the results showed 3 natural sheep cases were spa, 2 of 16 sheep were positive and others were negative, 16 goats were negative
本試驗還應用pcr技術，對培養的病毒進行檢測；並對自然感染spa的3例綿羊、隨機採取的16例山羊和16例綿羊進行了診斷，結果表明：自然感染的3例綿羊確實患有spa ，為陽性；隨機採取的16例綿羊中有2例為陽性，其餘14例為陰性； 16例山羊均為陰性，未感染spa 。 -
Using random primed gene walking pcr ( rpgw - pcr ), ctsox2 gene was cloned and sequenced from the digested genomic dna of trionyx sinensis. compared with the mouse and chicken sox2, ctsox2 shared 86 % and 91 % nucleotide homology respectively, and 93 % and 97 % amino acid identity respectively
天i消化過的中華鱉（ trio矛移優sinensis ）基因組dna中克隆了c眨治xz基因（ 72obp ） ，它與鼠、鳥的及戲2基因分別有85 %和91 %的核昔酸同源性， 93 %和97 %的氨基酸同源性。 -
Import control monitoring the antibody level, dead and sick bird monitoring, random screening test h5 rrt - pcr
入口管制監察抗體監察死亡及生病雀鳥抽查測試h5rrt - pcr -
The mutant pel - d92l was expressed in pichia pastoris gs115, sds - page detection showed that the expression product pel - d92l - gs is different from pel - gs, and its " yield decreased dramatically, the themostability of pel - d92l - gs is also different from the pel - gs, but their optimum temperatures are same. 3. directed evolution of pel through random mutagenesis mutagenesis pcr carried out in error - prone conditions was used on the vector psk - pel, using the oligos " beginning " and " end ", homologous to the 5 ' and to the 3 " ends of the gene of pel respectively
三、 pel基因的隨機誘變用易錯pcr方法對pel基因進行隨機誘變， pcr產物與ppic3 . 5k連接，轉化大腸桿菌，獲得的混合質粒電轉化畢赤酵母gs115 ， omm平板篩選適于低溫或對熱穩定的重組子，篩選獲得一株最活反應溫度、熱穩定性、發酵酶活均有提高的突變體pel - ep5 - gs ，其最活反應溫度為45 ，比野生型高出5 ； 40處理30min殘留活性為56 ，大大高於野生型的6 ；初始ph7 . 3528條件下培養72h ，發酵上清酶活為325u / ml 。 -
Similarity coefficient and genetic distances between two arbitrary individuals were caculated according to the data of experiments, the pedigree was constructed by two means ( they were upgma and parsimony ), and genetic diversity was discussedbased on selecting the best conditions of pcr with opg and opa primers from operon company, we obtained 163 bands using 24 random primers. in other words, 163 sites in crested ibis " genome were detected. the bands from 23 primers showed polymorphism. minimurn of polymorphic ratio was 0, maximum was 85. 71 %, and average was 48. 99 %. in the population which was composed by 37 individuals, most of genetic distances between two arbitrary individuals were less than 0. 1. the results showed that the degree of similarity among crested ibises was high while genetic diversity in the population was low accordingly
我們在對operon公司opg系列和opa系列引物進行優化篩選的基礎上，用24條隨機引物共擴增出163條譜帶，即檢測了朱?基因組中的163個位點，其中23條引物的擴增結果具有多態性；譜帶的多態比率最小為0 ，最大為85 . 71 ，平均為48 . 99 ；在由37隻朱?組成的種群中，任意兩個個體之間的遺傳距離絕大多數在0 . 1以下。這些結果表明，目前朱?個體間的相似程度很高，種群的遺傳多樣性水平較低。 -
The analysis of genetic diversity of 10 families in p. taiwanensis hayata. was carried out by rapd molecular markers, which may provide the theoretical base for breeding. 17 primers were screened from 206 random oligonucleotide primers and 38 polymorphic loci were obtained from pcr of 17 primers
利用rapd分子標記分析了黃山松10個家系的遺傳多樣性，為育種提供依據，從206個隨機引物中篩選出17個引物，獲得38個多態位點。
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