reading with expression 中文意思是什麼

reading with expression 解釋
有表情的朗讀
  • reading : n 雷丁〈姓氏〉。n 1 閱讀；讀書；講讀；朗讀，講讀會，讀書會；（議會議案的）宣讀。2 讀物。3 學識；...
  • with :
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. In comparison with genbank data, the homologies of the nucleotide sequence and amino acid sequence were as following : hc was 98. 4 % and 100 % ; ha was 97. 2 % and 99. 3 % respectively. 4. two recombinant prokaryotic expression vector were constructed which has complete open reading occlusing initation codon, leader signal peptide sequence and termination codon

    序列分析表明，所克隆獲得的基因與genbank中已經登錄的核苷酸和氨基酸的同源性分別為： hc98 . 4和100 ， ha97 . 2和99 . 3 ，證明本試驗蟲株與國外報道的同源性很高。
  3. In each competent training step, the writer put forward clear request with regard to the learning strategy : request on memorizing training - reading and inducing, memorizing the materials on the basis of comprehending ; request on comprehension training - abstracting and thinking, revising the previous knowledge and then learn the new ; request on operation training - concerning about political affairs, analyzing and evaluating ; request on expression training - thinking hard and practicing more, weigh the words of material

    其具體運用方式有兩種：正向訓練法和綜合訓練法。在每一種學科能力訓練中，提出學法指導的具體要求：識記能力訓練要求? ?閱讀歸納理解記憶；理解能力訓練要求? ?思考提煉溫故知新；運用能力訓練要求? ?關心時事分析評價；表述能力訓練要求? ?勤思多練字斟句酌。
  4. The eldest princess paused in her reading and stared at him in silence with dismay in her eyes. the second assumed precisely the same expression. the youngest, the one with the mole, who was of a mirthful and laughing disposition, bent over her frame, to conceal a smile, probably evoked by the amusing scene she foresaw coming

    年長的公爵小姐中斷了朗讀，默不做聲地用恐懼的眼睛朝他瞟了一眼那位年幼的公爵小姐，臉上沒有胎痣，卻流露出同樣的表情最年幼的小姐，臉上長著一點胎痣，天性活潑，滑稽可笑，她朝繃子彎下腰去，藏起了笑意，大概她已預見到即將演出一幕鬧劇，這使她覺得可笑。
  5. Some of the projects which have been launched include : " chinese literature on the net ", " inter - university multimedia special online project ", " speaking better english online ", " online reading projects ", " web thinking with dr. lee tien - ming ", " online with pai hsien - yung ", " hong kong colloquial expression ", " distinguished lecture series ", " cultural travel ", " healthy living ", " music fantasy " and " one minute s reading " etc

    香港電臺e - learning頻道，為大家提供文學藝術欣賞和語言學習的素材。已推出項目包括文學相對論多媒體創意廊speaking better english online網上讀書樂李天命網上思考我和白先勇的網上約會俗語趣談傑出學人學術講座健美學堂文化旅遊系列音樂的遐思及一分鐘閱讀等。
  6. In this research two full - length cdnas have been cloned by a combination of rt - pcr and 3 " - is1 - race with synthesized degenerate primers from young leaves of vicia faba l., pichia methanolica high - level expression systems of the genes have been constructed, and the milligram expressed protein was purified using probond resin purification system, which may result in further identification of the function of the aba binding protein. the full - length cdna of abp370 fragment is 3449 bp long and has an open reading frame of 2304 bp encoding 768 amino acids with 876 bp long 5 ' - utr, 369 bp long 3 ' - utr and poly ( a ) tail. the full - length cdna of abp640 fragment is 1012 bp long and has an open reading frame of 780 bp encoding 260 amino acids with 88 bp long 5 ' - utr, 144 bp long 3 " - utr and poly ( a ) tail

    3 - 5 - race擴增片段序列分析結果表明， abp370擴增片段的全長cdna為3449核苷酸，其中5非翻譯區為876個核苷酸， 3非翻譯區為369個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子tga ，含有一個編碼768個氨基酸殘基的開放閱讀框架（ 2304bp ） ； abp640擴增片段的全長cdna為1012核苷酸，其中5非翻譯區為88個核苷酸， 3非翻譯區為144個核苷酸並末端帶poly （ a ）尾巴，從起始密碼子atg至終止密碼子taa ，含有一個編碼260個氨基酸殘基的開放閱讀框架（ 780bp ） 。
  7. Sequence analysis indicated that the opening reading frame of ns1 gene is 1056nt in length, encoding 352 amino acids, identical to the sequence of strain sa 14 - 14 - 2 deposited in genbank. ns1 gene in the pmd18 - t - ns1 was cut by bamhi and hindiii, was cloned into the expression plasmid pet - 30b ( + ) treated with the same enzymes, resulting in a recombinant plasmid pet - 30b - ns1. the pet - 30b - ns1 was identified by pcr, restriction digestion, and sequencing

    通過序列分析結果表明， jev - ns1基因編碼區核苷酸序列長度為1056bp ，編碼352個氨基酸，序列分析和比較表明本實驗克隆到的ns1蛋白基因與genbank中收錄的疫苗株sa14 - 14 - 2的核苷酸序列一致，表明盡管該疫苗株在我國應用多年，但ns1基因並未發生變異，提示該疫苗株遺傳性狀比較穩定，是一株優良的疫苗株。
  8. Well, ill read it then, bagration seemed to say, and fixing his weary eyes on the paper, he began reading it with a serious and concentrated expression

    「喏，讓我來朗誦， 」巴格拉季翁好像說了這句話，他於是把那疲倦的目光集中在一張紙上，他裝出聚精會神的嚴肅認真的樣子朗誦起來。
  9. The following chapters, supported by examples from high school english books, deal with the differences between the two languages in modes of thinking, words and their meanings, syntax and text construction. the last chapter supplies two reports with examined data to conclude that above 40 % of the various drawbacks in the senior high students " english reading and writing are caused by the discrepancies in the mode of expression

    然後從英漢文化和思維方式的差異入手，結合中學課本中的實例，依次分析英語和漢語在詞語、句法和篇章方面的基本差異，最後一章通過兩份調查報告來驗證前人的理論，並得出自己的結論：高中生在閱讀和寫作中由於英漢表現法差異而引起的失誤在40以上。
  10. Initially, a polyclonal antibodies was used to screen the cdna expression library to isolate pf40 gene from millet. sequence analysis revealed that the pf40 gene contained an open reading frame ( orf ) encoding a protein with 75 % - 35 % sequence identity to the zips ( zinc or iron transporter proteins ) gene family. software analysis of the pf40 gene character showed that pf40 protein had a eight trans - membrane region structure, pf40 protein was also rich in leucine ( 18. 4 % ) and the protein was a hydrophobic protein

    序列分析表明該基因具有一個開放閱讀框（ openreadingframe ， orf ） : genbank中查詢該基因編碼的蛋白與zips （ zincorirontransporterproteins ，離子通道蛋白）基因家族的蛋白有75 - 35的相似性；軟體的分析結果顯示該基因編碼的蛋白為一個有8個跨膜區的跨膜蛋白； pf40蛋白為疏水蛋白，以上的特徵也許是pf40基因發揮作用的基礎。
  11. We sequence the inserted gene fragment of the indentified recombinant clone. the result is : angiostatin gene orf ( open reading frame ) links with the orf in expression vector correctly. but the first base of the codon aaa coding for lys414 in plg kringle 4 domain mutates from a to g which leads lys change to glu

    隨后取通過上述鑒定的重組克隆菌，對重組子插入片段測序，結果為： as基因開放讀碼框與表達載體的讀碼框正確匹配相連，但在其kringle4區相當于編碼plg的lys ~ （ 414 ）密碼子aaa的第一位堿基由a突變為g ，導致相應的氨基酸殘基突變為glu 。
  12. The results showed that the two isolates have more than 95 % homology with the snv strain. the env gp90 was amplified from the snv strain by pcr and was cloned into the downstream of gst gene in pgex - 6p - l vector according to the correct open reading frame, and this recombinant was transformed into bl21 for expression

    結果發現，在核苷酸水平上，參考株snvenv基因與兩株中國分離株的同源性分別為97 . 7和95 ，而在氨基酸水平上，其同源性為96 . 9和92 . 7 。
  13. After the pbd i and pbd ii gene ligated with the expression vector pinpoint ? a - 3, the recombinated plasmid ppd - 1 and ppd - 2 was transformed into jm109 strains, 4 positive clones were screened by restriction enzyme analysis, dna sequencing showed that two out of 4 positive clones inserted sequence of the constructed plasmid, which was the same as that of pbd - i and pbd - ii gene respectively, and its reading frame was correct, thus its could be used to express fusion protein

    將pbd - 、 pbd -基因與表達載體pinpoint ~ （ tm ） xa - 3連結后獲得的重組質粒ppd - 1 、 ppd - 2轉化于大腸桿菌jm109中。經抽提質粒、酶切分析及pcr擴增，分別篩選到4個陽性克隆，將其中二個陽性克隆由測序分析，證實1個含pbd i基因片斷， 1個含pbd 11基因片斷，且閱讀框正確，可用於融合蛋白表達。
  14. Recognition and analysis of human faces can be widely used in such fields as personal identification recognition, safety inspection, human - computer interaction, expression analysis, lip - reading, etc. as the basis, necessity and prerequisite of human face recognition and analysis, human face detection with the help of computer attracted people ' s attention at a very early time

    人臉的識別和分析可以廣泛應用在個人身份識別、安檢、人機交互、表情分析、唇讀等應用中。計算機人臉檢測作為人臉識別、人臉分析等應用的前期步驟和首要條件，很早就受到人們的關注。
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