recombinant enzymes 中文意思是什麼
-
When both genes were co - expressed in e. coli, the activity of ppsa varied from 2. 1 - 9. 1 fold comparing to control, but the activity of tkta was relatively stable ( 3. 9 - 4. 5 fold ). whatever the two genes were expressed respectively or cooperatively, both could promote the production of dahp, the first intermediate of the common aromatic pathway, but co - expression was more effective on forming dahp and screened ppt - and ptp - as more effective. the results demonstrate that co - expression of ppsa and tkta can improve the production of dahp, and what ' s more, when multigenes co - expressed, the recombinant which has coordinated enzymes activity is optimum
莽草酸途徑的最優化和整體調控基因csra的敲除正是上述改變的分子基礎，同時也為三種芳香族氨基酸的基因工程菌的構建打下了基礎; 7 .在國內外首次實現了共同途徑限制性底物關鍵酶ppsa刁無『及arog與分支途徑關鍵酶基因phea的串聯高效表達，所構建的重組質粒ptga ，其ppsa 、 tkta 、 arog 、 cm和pd的酶活分別比對照提高了3 、 2 、 2 ， 5 、 4 、 2 . 3倍，且其酶活比較協調一致; 8 .將ptga導入到篩選的基因敲除和基因替換菌株大腸桿菌31884 c甲b中，搖瓶發酵證實比以往所構建的基因工程菌株具有較高的phe產量和糖轉化率率，分別為0 . 448 %和22 . 4 % 。 -
Sequence analysis indicated that the opening reading frame of ns1 gene is 1056nt in length, encoding 352 amino acids, identical to the sequence of strain sa 14 - 14 - 2 deposited in genbank. ns1 gene in the pmd18 - t - ns1 was cut by bamhi and hindiii, was cloned into the expression plasmid pet - 30b ( + ) treated with the same enzymes, resulting in a recombinant plasmid pet - 30b - ns1. the pet - 30b - ns1 was identified by pcr, restriction digestion, and sequencing
通過序列分析結果表明， jev - ns1基因編碼區核苷酸序列長度為1056bp ，編碼352個氨基酸，序列分析和比較表明本實驗克隆到的ns1蛋白基因與genbank中收錄的疫苗株sa14 - 14 - 2的核苷酸序列一致，表明盡管該疫苗株在我國應用多年，但ns1基因並未發生變異，提示該疫苗株遺傳性狀比較穩定，是一株優良的疫苗株。 -
Chunna yu, linya you, su zeng. studies on the recombinant drug metabolizing enzymes. the eighth national conference on drug and xenobiotics metabolism in china, dalian, october 2006
俞春娜，尤琳雅，曾蘇，重組藥物代謝酶的研究與應用，第八次全國藥物與化學異物代謝學術會議，大連， 2006年10月。 -
To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。 -
Sensitive assays require a highly purified enzyme with a high turnover number, and a low detection limit for the reaction product. diagnostic enzymes produced by recombinant dna technology, which increased yield and quality of enzyme and simplified preparing process, improved the development of eia greatly
利用基因工程生產診斷用酶，使酶的純度大大提高，產量及質量均較自然來源酶好，簡化了制備程序，大大促進了酶免疫分析技術的發展。 -
The polymers also hinder the protein from being cut up by enzymes, and researchers are investigating whether they might diminish immune reactions experienced at times by patients who are injected with the recombinant epo
此外，注射重組epo的病患有時候會出現免疫反應，目前研究人員也正在調查這些聚合物是否可減少這種情況的發生。
分享友人