recombinant rna 中文意思是什麼
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Constructing cdna expressing library of erythrocytic plasmodium falciparum from hainan : the total rna was obtained by using. triplix kit. a modified oligo ( dt ) primer ( cds ffi pcr primer ) was used in the single - stranded ( ss ) dna synthesis reaction. the ss - dna was reversely transcripted from total rna. double - stranded ( ds ) cdna was amplified by long - distance ( ld ) pcrafter the digestion with proteinase k and sfi i, the cdna with no less than 200bp was collected and purified by glass - milk kitthe library was constructed after the ligation of cdna to tiplex2 phage particle packaged with the packaging extract system in vitro. a high titer and high recombinant ratio of cdna library was constructed
構建惡性瘧原蟲海南株紅內期cdna表達文庫提取紅內期惡性瘧原蟲海南株總rna ，直接以總rna為模板使用cdna文庫構建試劑盒，首先反轉錄合成ss一dna ，再擴增合成ds一dna （ cdna ） ，對擴增產物用蛋白酶k消化及左z丁i酶切，抽提蛋白、去除rna后，用玻璃奶試劑盒純化、回收20obp以上的片斷，經與載體連接再用蛋白包裝物包裝后形成未擴增文庫，最後擴增完成惡性瘧原蟲海南株紅內期cdna表達文庫的構建。 -
The patterns of sds - page indicated more than 30 % of recombinant proteins could be obtained from the extract of e. coli bl21. furthermore, library of recombinant phage antibodies was constructed from total rna isolated from spleen of the female balb / c mice immunized by bull sperm
首先用牛精子免疫雌性四周齡balb c小鼠，從其脾臟組織分離總rna ，應用重組噬菌體抗體庫技術，構建了一個針對牛精子的噬菌體抗體文庫。 -
Reversal of the multidrug resistance in human hepatocellular carcinoma cells by recombinant adenoviral delivery of the mrp antisense rna in vitro
逆轉人肝癌細胞多藥耐藥表型的體外實驗研究 -
Secondly, we established a rdrp reaction system. then the recombinant ns5 protein was added to the system and its rdrp activity was confirmed after the newly synthesized rna showed resistant to rnase a. furthermore, we found that the recombinant ns5 could specifically bind antibodies in anti - den serum indicating the immunoreactivity of the recombinant ns5 and the irnmunogenicity of den ns5
重組nss蛋白的rd即反應和反應產物的rnasea敏感性實驗表明，所得重組nss蛋白具有良好的rdrp活性，能以亞基因組rna為模板合成與之互補的rna ，新生鏈是與模板鏈相結合的雙鏈形式存在於rd即反應體系中。 -
The mutants were transfected into pc 12 cells respectively to probed the ability respondence to gdnf stimulation and the interaction with ret by immunoprecipitation, c - ret phosphorylation and immunoblotting assays. the main results are as follows : 1. expression and purification of recombinant rat gfral protein to obtain recombinant gfral and study its biological activity, the cdna encoding the mature rat gfral was isolated using rt - pcr with total rna extracted from newborn s
純化和復性后的重組gdnf蛋白，可顯著增強pc12一gfral一ret工程細胞的存活和分化;對pc12一ret工程細胞沒有任何作用;對pc12一gfral的存活和分化作用11中英文摘要顯著強于對pc12一ret的作用，但也顯著低於對pc12一gfral一ret細胞的作用。 -
( 3 ) establishing a new method which can quantitatively determinate telomerase activity. 1. human telomerase rna component ( htr ) gene was cloned from hela cells using gene engineering technology. the htr gene was then reverse inverted into retrovirus vector plncx and constructed an antisense recombinant plasmid plncx - atr
將htr基因插入逆轉錄病毒載體plncx中，構建了htr基因的反義重組質粒plncx - atr和正義重組質粒plncx - tr ，測序結果表明符合預期要求，為下一步實驗奠定了基礎。 -
Ii ) two fragments ( about 240bp and 130bp ) were amplified from human keratinocytes total rna by rt - pcr. the recombinant pm - hpabl and pm - hpabs plasmids were constructed by inserting 240bp and 130bp pcr products into pmd 18 - t vector, respectively. the recombinants were identified by restriction enzyme analysis and dna sequencing, iii ) two orfs ( > 100bp ) were found in the insert sequence of pm - hpabl
應用smartpcr試劑盒和簡並引物從人皮膚角質形成細胞cdna中擴增到長分別為24obp和13obp左右的2種片段，將它們插入pmd18一t載體，用酶切法初步篩選陽性重組子pm . hrabl和pm一hrabs ，對陽性重組子進一步作測序鑒定。 -
Then we conducted in vitro gene transfer, transfecting p388d1 with recombinant eukaryotic vector and infecting hela with recombinant adenoviruses respectively, in order to observe inhibition of constitutive or inducible cii ta gene expression by introduced complementary antisense rna of c iita and consequent do wnregulation of class ii mhc molecules expression
結果顯示：含反義c ta的真核表達載體轉染組成型mhc -類分子表達p388d1細胞株，經抗生素抗性篩選后表達mhc -類分子的細胞陽性率比對照組下降65
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