recombinant protein 中文意思是什麼
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The target gene was then subcloned into plasmid vector and induced by iptg for its expression. after that, the recombinant protein was purified and used for the identification and characterization of its immunogenicity. the effect of the induced specific ige antibody on the hepatic granuloma formation and fibrosis after challenge infection with schistosome cercariae was evaluated
Niptg誘導表達，產物沉澱中於約45kda處顯見高合蛋白表達帶， westernblot分析表明，該蛋白帶可被篩庫血清中特異性lge抗體識別；而載體本身表達的26gst蛋白帶則否。 -
The inclusion bodies of recombinant protein were purified with washing buffer consisting of various urea ( 2mol / l and 4mol / l ) for several times, and then dissolve the fusion protein in the denature buffer using 8mol / l urea as denaturant
用含有2mol l和4mol l尿素的包涵體洗滌液洗滌包涵體，在37條件下，洗去了大部分菌體蛋白及其它核酸物質。用8mol l尿素作為變性劑溶解包涵體，包涵體在8mol l尿素中的溶解性非常好。 -
17 - hsd8 / pcdna3. 1 recombinant protein expressed in hek 293 cells and it can slightly interconvert estrone to estradiol. moreover, it can catalyze the conversion from testosterone to androstenedione. however, 17 - hsd3 did n ' t show any conversion to all the substrates checked in this study
而17p一hsds轉染hek293細胞表達的17p一hsds / pcdna3 . 1重組蛋白能微弱地催化el和ez之間的相互轉化，同時也能催化從t到a的氧化反應。 -
The recombinant protein with the molecular weight of 76 kd was mainly expressed as inclusion bodies in e. coli, which was identified by sds - page
Sds - page證明，重組融合蛋白的分子量約76kd ，並主要以包涵體的形式存在。 -
In an earlier study, genotype 1 he recombinant protein accine was shown to be effectie in nonhuman primates
在一項較早的研究中， he基因型1重組蛋白疫苗對非人類靈長目有效。 -
The results shows that the vitro expressed protein of n gene by recombinant plasmid vector in the e. coli maintains anigenicity of tgev the recombinant protein was purified acconiing to the vector self characteristic ( hisk a polyhishdine tag introduced at the amino - acid terminus of the nucleoprotein allowed for the purification of protein by nickel - chelate dsity chromataography we explored all possibilities of pedcation and gained the modified purification method. several conditions, which include diffend ph buffer and concelltheion of imidazole, were selected to purify recombinan nucleorotein
根據載體pproexhtb含有（ his ） 6特點，將融合蛋白進行純化，在純化過程中經各項條件的探索，確定為在裂解液中含有1mmpmsf的條件下，分別經過2倍體積的buffera和bufferb洗脫后，再收集ph5 . 9 ，含有80mmol / l咪唑的1倍體積bufferc洗脫液，可得到純化的融合蛋白。 -
Fusion protein expression system can overcome those problem, increased the yield of yield of recombinant protein in e. coli. this remarkable increase in protein yield was thought to be due to protection of the target protein from proteolysis, improved folding, and efficient mrna translation. fusion protein also make the detection and purification easy, is a good strategies for achieving high - level expression of genes in escherichia coli
小分子量異源蛋自在人腸桿菌的表達受mrna不穩定、翻譯起始效率低、易被蛋自酶降解等因素的干擾，較難獲得高效表達，通過與已高表達的蛋自融合表達可以克服以上問題，可以使大多數蛋白獲得高效表達。 -
To investigate the mechanism of trefoil factor 3 on the gastric intestine epithelial restitution, the recombinant human trefoil factor 3 was added to human colonic tumor cell and the proliferation effect was examined by mtt assay. the recombinant protein didn t promote the proliferation of the hct cells at low density of 0. 010. 05 g l and only has weakly proliferation effect at density of 0. 10. 2 g l. 1 g l of the recombinant protein could significantly promote the cell migration of hct cells when added to the monolayers cells
將重組人三葉因子3 trefoil factor 3 ， tff3作用於人結腸腫瘤細胞，研究重組蛋白對細胞增殖的影響，結果發現該蛋白在較低的濃度1050 mg l下對細胞的增殖基本沒有影響，在100200 mg l濃度下該蛋白對細胞僅有微弱的刺激作用，提高濃度對細胞增殖作用沒有改變。同時研究了tff3對損傷的單層結腸腫瘤細胞遷移的影響，發現tff3對細胞有明顯的促進遷移作用。 -
Deficiency of apoe may promote to produce and develop atherosclerotic lessions. the apoe gene - targeted mice will result in marked regression of both early and advanced atherosclerotic lesions by injected apoe recombinant protein, or by transfected adviral vector with apoe cdna to express human apoe transgene in liver, or by transplantation of bone marrow with normal rat apoe gene. this demonstrates that apoe gene and its expressing product can inhibit progression of atherogenesis. apoe3 has a more effective prevention from as than apoe2 and apoe4
Apoe的缺失可促進動脈粥樣硬化的發生發展，給apoe基因敲除鼠反復注射apoe重組蛋白、在肝組織中用腺病毒載體表達apoe蛋白、移植帶有正常apoe基因的小鼠骨髓，都能使apoe基因敲除鼠的動脈粥樣硬化得到回復，表明apoe基因及其表達產物對動脈粥樣硬化的發生具有抑制作用， apoe _ 3對動脈硬化的阻抑作用要比apoe _ 2和apoe _ 4都明顯。 -
During the different time point, we retrieve the cell supernatant and through the electrophoretic analysis and immunoblot of the recombinant protein indicated that the cell which transfected the recombinant eukaryotic gene expressing vector could synthesis and excrete the peptide effectively
提取培養細胞總rna反轉錄cdna測序結果表明人生長激素基因組dna在家蠶bmn細胞內能正確轉錄，剪接。蛋白質電泳分析和免疫學檢測證明轉染細胞能夠有效合成並分泌hgh蛋白質。 -
This paper is a study on the expression of the protective gene of bont / a in the prokaryotic and eukaryotic. it indicates the possibility to produce the recombinant protein in quantities. it has also laid down a good foundation for the further research on vaccine or antibody of bont / a
本論文進行了人工合成的a型肉毒毒素hc段基因在原核和真核表達系統中的表達研究，使制備大量bont a保護型抗原的重組蛋白成為可能，為進一步進行a型肉毒毒素的疫苗或抗體研究奠定了一定的基礎。 -
Baculovirus / insect cell system has been widely used for recombinant protein production, but traditional system eventually resulted in cell lysis, so that the expressed recombinant protein was lost into medium
摘要:桿狀病毒/昆蟲細胞系統已經被廣泛的應用在重組蛋白質的生產上，但傳統的桿狀病毒感染后會造成細胞溶裂，而使得表現出的重組蛋白質流失到培養基中。 -
Study on application of recombinant protein of lymphocytic choriomeningitis virus
重組蛋白抗原應用研究 -
The recombination vectors were transformed into host strain of bl21 and induced with iptg. all the recombinant protein was expressed into inclusion body. the recombinant protein was identified with sds - page and westen - blot and purified through elution method. the muticlone antibody was got by immuning the rabbit with the purified protein
把重組質粒轉化入表達受體菌bl21 ，經iptg誘導后都獲得了表達，且都以包涵體的表達形式存在，用sds - page和western - blot的方法對重組蛋白進行了鑒定。 -
Although much more research will be needed, treatment with recombinant protein c could offer a new way of interrupting the progression to sepsis and organ failure in high - risk patients
此項研究之所以重要就是因為它確定了一種對伴有嚴重敗毒癥和多器官衰竭患者進行確診的簡單策略" 。 -
2. the molecular weights of the recombinant protein of the cadherin repeated domain 4, 5, 6 of bt - r3 were about 42kda and 45kda ( with his tag ) respectively. both expressed products could not specifically bind to bt toxic protein crylab
表達得到了bt - r3受體的第4 ， 5 ， 6個鈣粘蛋白結構域重組蛋白，其分子量約為42kda和45kda （帶histag ） ，均不能有效地結合bt毒蛋白cry1ab 。 -
We paid our main attention to cloning tb22kda gene from tartary buckwheat, expressing the structure gene and getting the purified recombinant protein, and these provide foundation for father study on the relationship between structure and function of the allergenic protein tb22kda, the orientation of the corresponding epitope and the exploration of allergenic reaction mechanism
本研究的目的在於分離克隆苦蕎中的主要過敏蛋白（ tb22kda ）基因，並表達純化出其結構基因編碼的蛋白。從而為進一步研究過敏蛋白tb22kda的結構與功能，尋找其中的抗原決定簇，探討過敏原與其相應抗體相互作用機理提供依據。 -
Conclusion constructed the high - level expression clone of echistatin in e. coli. the expression of recombinant protein is higher, it make the further study of echistatin feasible
結論成功構建了echistatin的原核高效表達克隆，表達量高於現有國內外研究水平，為echistatin功能及相關疾病的研究奠定了基礎。 -
The recombinant protein exhibited specific binding to antiviral antibodies of h5 subtype and has no cross - reaction with h7 and h9 subtype
分別用親和層析以及電洗脫兩種方法對gst - ha1融合蛋白進行了純化，都獲得了理想的純化結果。 -
Once the recombinant virus has been i - dentified, we amplified the p - 1 stock to attain the large scale, high - titer viral stock in order to initiate expression studies. the recombinant angiostatin was produced from spodoptera frugiperda 9 ( sf9 ) insect cells infected by the high titer virus stock we have prepared. the time course for expression of recombinant protein was detected by sds - page and western blot, which can determine the optimal multiplicity of infection ( moi ) and the appropriate time of harvest for the protein
表達重組蛋白angiostatin ：用制備好的帶有矗s標記的融合性angiostatin基因的高滴度重組桿狀病毒貯存液感染草地貪夜蛾sfg細胞，用sds page電泳和hsternblot對感染不同時間後分泌的重組蛋白做時間表達分析，依此確定最適的感染復數（ moi ）和感染時間，以達到重組蛋白表達水平最適化，而後大規模進行重組蛋白的表達， sds page用來分析重組蛋白， westernblot用來在蛋白表達水平低的情況下檢測表達的特異重組蛋白。
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