regeneration under selection system 中文意思是什麼

regeneration under selection system 解釋
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  • regeneration : n. 1. 再生,新生。2. 悔悟。3. 改造,革新。4. 回收。5. 【化學】交流換熱(法);【物理學】正回授[反饋]放大。
  • under : adv 1 在下,從屬著,服從著。 bring under get under keep under 等〈參看各該動詞條〉。 The ship wen...
  • selection : n. 1. 選擇;挑選;選拔。2. 拔萃;選擇物;精選物[品];文選。3. 【無線電】分離,(自動電話)撥號。4. 【生物學】選擇,淘汰。
  • system : n 1 體系,系統;分類法;組織;設備,裝置。2 方式;方法;作業方法。3 制度;主義。4 次序,規律。5 ...
  1. Mature embryo - derived calli of japonica rice ( oryza sativa l. ) cultivars nipponbare were transformed using agrobacterium tumefaciens strain agl1 carrying a binary vector pcas04 harboring the marker gene, neomycin phosphotransferase gene ( nptii ), driven by a promoter from the ubiquitin gene in maize, a promoterless p - glucuronidase gene near to the left border of t - dna for trapping gene and a strong promoter, rice actin - gb promoter, near to the right border of t - dna as activation tagging. in this system, co - cultivation was simplified, special selection stages and pre - regeneration stage were omitted, the whole process was almost under continuous light at 30 ? except co - cultivation and transgenic plants began to generate only after 7 weeks calli were induced

    在一步轉化系統中,光照高溫條件下培養的水稻愈傷組織從誘導開始經過4周時間就可以達到轉化實驗的要求,並且簡化、優化了整個共培養過程,省去了一篩、二篩和預分化步驟,只用7周的時間就可以初步得到再生轉化植株;共191塊愈傷組織得到125塊抗性愈傷組織,轉化頻率達到65 . 4 ,最後共得到99棵獨立來源的再生植株,抗性愈傷組織再生頻率達到79 . 2 。
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