region sequencing 中文意思是什麼

region sequencing 解釋
切削區域的順序
  • region : n. 1. 地方，地域，地帶；地區；行政區，管轄區，區；左近，鄰近；（大氣、海水等的）層，界，境。2. 【解剖學；動物學】（身體的）局部，部位。3. （學問等的）范圍，領域。4. 〈罕用語〉天空。
  • sequencing : 測序，序列測定
  1. The phylogenetic analysis of 15 geotrichum strains based on 26s rrna gened1 d2 region sequencing

    2區序列分析的15株白地霉分子分類學研究
  2. By elisa analysis, inhibition of binding of clq with the c ! q receptors on u937 cells and competitive inhibition of binding of clq with aggregated immunoglobulin g b y selected phage clones, and dna sequencing, a number of similar, but not identical, sets of sequences of clq - binding clones were identified. the deduced amino acid sequences of selected 9 peptides are wyegpftlytwp, hwdpfslsayfp, ltqhnspffllp, tsnpfflwypqp, qtpfqlw, npfnwts, spfxlts, fltwldp and fstflyp. they show significant efficiency to inhibit the binding of clq with the clq receptors on u937 cells and / or aggregated immunoglobulin g, which suggest that the selected peptides contain the modeling epitopes of clq receptor to bind the collage - like region or igg to bind the head domain of clq

    然後，採用噬菌體肽庫技術，以c1q為釣餌蛋白，從12肽庫和環7肽庫中親和篩選能與c1q結合的噬菌體克隆，經elisa 、 u937細胞配體結合抑制試驗、 aigg競爭抑制試驗及dna測序，獲得了9個具c1q抑制活性克隆的dna序列，其相應的氨基酸序列為： wyegpftlytwp 、 hwdpfslsayfp 、 ltqhnspffllp 、 tsnpfflwypqp 、 qtpfqlw 、 npfnwts 、 spfxlts 、 fltwldp 、 fstflyp ，它們可能模擬c1qr和或igg的c1q結合表位並抑制c1q的活性。
  3. A few years latter the research showed, vp2 hydrophilic region and antigen variant and virulence of ibdv had very affinity. in order to lucubrate difference of virulence and antigen, and validate correntness of sequencing result, the test adopted sandwich enzyme - liked immunosorbent assay ( elisa ) and antigen caprure - elisa to determine virulence and antigen. and validate correntness of above - mentioned result

    而近幾年的研究表明， vp2高變區與ibdv的抗原變異和毒力強弱有密切的關系，為進一步了解強弱毒株毒力和抗原的差別，並進一步證實測序結果的正確性，本試驗採用雙抗夾心elisa和ac - elisa的方法分別對其毒力和抗原進行了測定，並通過動物試驗證實了，其結果與序列分析結果基本一致。
  4. In order to further investigate the role of axudl in human tumor carcinogenesis and the potential association between the axudl gene expression status and the stimulation of transforming growth factor beta in human cancers, the present study was performed in three aspects as follows : ( 1 ) cloning full length enconding region cdna of axudl and construction of eukaryotic vector that expression the fusion protein of axud1 and influenza virus hemagglutin ha epitope tag ; ( 2 ) exploring the time and dose effects of tgf - 1 on the expression - of axudl gene in hepg2 hepatoma cells and spc - a1 lung carcinomas cells, and studying the effects of overexpression of axud1 on the expression of cell cycle and apoptosis related protein in hepg2 hepatoma cells ; ( 3 ) construction and expression of human axudl in e. coli m15. the following main results and conclusions can be obtained from the present study : 1. the full length ecnoding region of human axudl cdna from human peripheral blood lymphocytes was successfully cloned using one step rt - pcr method, and constructed into a eukaryotic expression vector which can be expressed a ha - axud1 fusion protein with axud1 and influenza virus hemagglutin ha epitope tag. the recombinant plasmid was identified by polymerase chain reaction, restriction endonuclease maping and sequencing, this expression vector might be instrumental to further study the function of axud1 protein in tumor cells

    為了進一步研究axud1在人類腫瘤發生中的作用及axud1基因的表達狀況與tgf -介導的信號通路的關系，本實驗研究分為三個部分： （ 1 ） axud1基因cdna全長編碼區的克隆和ha表位標記的axud1基因表達載體的構建； （ 2 ）探討肝癌細胞hepg2和肺腺癌spc - a1細胞中tgf - 1誘導的axud1基因表達的時間、劑量效應以及誘導表達的可能機理，並研究axud1的過表達對細胞周期和細胞凋亡相關蛋白表達的影響； （ 3 ） axud1原核表達載體的構建及其在大腸桿菌中的表達。本實驗的主要結果和結論如下： 1利用一步法rt - pcr成功地從人類外周血淋巴細胞中克隆出axud1基因編碼區cdna ，並將其構建入真核表達載體中，編碼的ha - axud1融合蛋白帶有流感病毒凝血素ha的表位標記肽段。
  5. Sequencing and genbank blasting demonstrated that these four had the same sequences and encoded part of arabidopsis choloroplast carbonic anhydrase ( ca ). the coding region contained 251 amino acid, from n terminal amino acid 86 to c terminus

    到genbank進行序列比對，結果表明它們編碼擬南芥葉綠體碳酸酐酶（ ca ）基因，編碼區含有部分ca序列，從n端第86位氨基酸至c末端，共251個氨基酸。
  6. Cloning and sequencing of cucumis melo fruit - specific expression cucumisin gene promoter region

    甜瓜果實特異性表達黃瓜素基因啟動子區的克隆和序列分析
  7. In order to get small molecular g - protein rab3a, which serves to further investigate the interaction between rab3a and other proteins, we amplified the full coding region of rab3a cdna by polymerase chain reaction, using human placenta total cdna as template. the pcr products were recovered from gel electrophoresis and cloned into bamhi xhoi site of vector pyestrp2. the result of sequencing indicated that rab3a insertion fragment included its initiation and termination codons in 5 - and 3 - terminal, respectively

    為了獲取全長的小分子g -蛋白rab3a ，以用於研究rab3a與其他蛋白相互作用關系，本實驗以人胎盤總cdna為模板， pcr擴增到人rab3a cdna全編碼區。產物回收后克隆于質粒pyestrp2的bamhi xhoi位點，測序結果表明，本實驗獲得的rab3a cdna包含了起始和終止密碼子。
  8. We obtained two positive clones after screening. sequencing and blasting results showed that the two clones had the same sequences and encoded part of ked like protein in arabidopsis. the coding region was from n terminal amino acid 240 to c terminus

    測序及序列比對結果表明二者序列相同，為ked樣蛋白的部分編碼序列，編碼部分從n端第240位氨宋林夜:利用酵母雙雜交系統篩選與g蛋白及cam相互作用的新型蛋白基酸至c末端，共207個氨基酸。
  9. The result of sequencing analysis shows that the coding region of the ba - dfe mature peptide has 825bp in length and encodes 275 amino acid residues, which has a n - terminal amino acid sequence identical to that of the purified ba - dfe determined by edman method, indicating that the cloned fragment was indeed as expected

    測序結果發現，其成熟肽編碼區有825bp ，編碼275個氨基酸殘基，推導的n -端序列與測得的該酶n -端序列完全一致，證明了m川人學博：學位論文該片段確實是ba dfe成熟肽編碼區。
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