repeated sequence (dna) 中文意思是什麼
repeated sequence (dna)
解釋
重序列-
And the intron had a lot of gt repeated sequence. the dna and protein sequence of this gene was analyzed using the bioinformatics tools. two functional domains were found in the protein
運用生物信息學手段對3一磷酸甘油脫氫酶基因核酸以及蛋白質序列做出了分析，發現這個基因編碼兩種功能的結構域，磷酸化酶結構域和3一磷酸甘油脫氫酶結構域。 -
Objective : construct high - level expression system of echistatin in e. coli methods : obtain amino - acid sequence of echistatin from genebank database. considering the bias of usage of 61 available aminoacid codons in e. coli, design the coding sequence of echistatin, synthesize the dna sequence chemically, get single copy coding gene and repeated two copy coding gene of echistatin. insert the sequence into expression vector pbv220, and more, we construct fusion expression clone of echistatin with pcr, identify the recombinant vector by dna sequencing
目的構建蛇毒鋸鱗蝰素（ echistatin ）的原核高效表達體系方法由genebank數據庫檢索蛇毒鋸鱗蝰素（ echistatin ）的氨基酸序列，結合大腸桿菌蛋白質合成體系對氨基酸密碼子使用的偏愛性，設計了echistatin編碼基因，體外人工合成編碼基因dna片段，通過適當的限制性內切酶位點插入表達載體pbv220 ，分別構建了echistatin的單拷貝表達克隆、雙拷貝串聯表達克隆；進一步通過pcr技術構建echistatin的融合表達基因克隆。
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