resistant target 中文意思是什麼
resistant target
解釋
有抵抗能力的目標-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
Detection of transgenic plants. the pcr assay of kan - resistant plants showed that the target gene had been integrated into tobacco accompanying with npt ii gene
轉基因pcr檢測pcr擴增npt11基因，證明thaumatin基因己導入煙草中，轉化率為引 -
The cowpea trypsin inhibitor ( cptt ) gene is testified as a broad spectrum insect - resistant gene at present and its application in insect - resistant botanic transgenic engineering only after s / gene. the cpti transgenic plant developed rapidly for it ' s broad spectrum insect - resistant character and the target insects are uneasy tolerance to it
豇豆胰蛋白酶抑制劑（ cpti ）基因是目前在植物抗蟲基因工程中應用僅次於bt基因的廣譜性抗蟲基因。鑒於它抗害蟲的廣譜性和靶標昆蟲不易對其產生耐受性的優點，轉cpti基因植物得到了迅速的發展。 -
However it may be resisted by the innovator, innovative target and surroundings etc. the accomplishment of education innovation needs innumerable conditions ( namely the required innovational conditions ). in reality, the conditions are not idealized enough to the innovation. some of the conditions may be helpful to the innovation, while some are resistant elements which can be divided into agent, management, target, client and environment included social factors ( such as politics, economics, culture and population ) and natural factors ( geographical environment )
據阻抗因素與改革活動之關系，有人將阻抗分為五大類型，而每一類阻抗又有若干子阻抗因索：改革代理人（昭ent ）囚素；改革管理因素；改革對象o呷et ）囚累；改革當事人（ client ）囚索；改單的環境囚索，又分社會因素（如政治、經濟、文化和人口）和自然囚索（如地理環境） 。 -
7 herbicide resistant plants were obtained. pcr, pcr - southern blotting analysis showed that the target genes were integrated into the plant genome. the segregation ratio in t1 of two examed transformants was 3 : 1
通過對標記性狀? ?除草劑抗性的活體及離體檢驗（氯酚紅法） 、目的基因pcr擴增、 pcr - southern雜交等方法證實目的基因已整合到小白菜基因組中。 -
After regeneration experiment, two cultivars, cut flower shanchengzhiguang and japanese xiaohuang, were selected as target materials for genetic transformation in this paper. sag - ipt prolong the senescence of leaves. armed with sag - ipt, transgenic cut flowers with storage resistant, longer shelf life and longer flower period may be obtained
所選用的外源基因為抑制葉片衰老的sag - ipt基因，以期得到耐貯運、瓶插壽命長的切花菊山城之光和抗衰老、花期長的綠化小菊日本小黃。 -
T. identification of charactrization of transgenic mustard plants the putative transformant regeneration plants were assayed by pcr and pcr - southern blot analysis. both analysis the target bands were observed. so the integration of the cpti gene into mustard genome dna was confirmed. the result of insect - resistance showed that the transgenic plants are more resistant than non - transgenic plants
轉基因芥菜植株的鑒定取再生苗的葉片提取dna ，進行pcr擴增和pcrsouthernblot分析，轉化再生植株大部分呈陽性，而非轉化的再生植株均為陰性，證明cpt基因已存在於芥菜基因組中。 -
Introduced infected by the turnip mosaic virus. there are many useful objective genes can be used in vegetable genetic transformation, but the research work of chinese cabbage genetic transformation is little. the reaserch on transforming chinese cabbage using agrobacterium - med ated method has n ' t been report at home and abroad. ln the test, tumv - cp gene was transferred into chinese cabbage ( brassica campestris l. ssp. pekinensis ) via agrobacterium - mediated method. a high effective regeneration and genetic transformatin system has been established, the detection by the method of molecular biology, has proved that the regenerative plants are transgenic plants and the target genes have been expressed transgenic plants partly. meanwhile transgenic progenies were traced and investigated so that heredity, stability and expression of target gene were researched. the virus resistant, stable plants were expected to obt ain so that theoretical base can be established for chinese cabbage breeding by gene engineering
利用農桿菌介導法轉化大白菜抗病基因的研究工作在國內外未見報道。本課試驗採用農桿菌介導法將tumv - cp基因導入大白菜中，建立了高效的大白菜離體再生、遺傳轉化體系，並對轉基因植株進行分子生物學檢測，證實得到的再生植株為轉基因植株，目的基因已在部分植株上表達。同時，對轉基因植株的後代進行檢測，分析該基因所控制性狀的遺傳穩定性以及基因表達情況，為大白菜基因工程抗病育種提供理論依據。 -
The bfr combat boot tested easily met satra s recommendation for highly water - resistant footwear, exceeding the target number of flexes by a factor of more than 30 times
Satra建議將達到6 ， 000次曲折不滲水的鞋子視為「防水」鞋，將達到15 ， 000次曲折不滲水的鞋子視為「高度防水」 。 -
With the development of the worldwide economy, takeover has become an irresistible trend. a target corporation, as the object of the takeover, always takes some resistant actions, which are known as the anti - takeover actions of target corporations
目標公司反收購行為是被收購公司為防止公司控制權的轉移，而針對收購，尤其是敵意收購採取的旨在預防或防止收購者收購本公司的行為。 -
Continuing health monitoring or regular condition assessment of important bridges is necessary to ensure that the bridges function well. in order to do that, a baseline finite element model of the target bridge is often indispensable not only for complex dynamic response analysis ( such as seismic analysis, wind - resistant stability analysis, train - bridge vibration analysis and so on ), but also for health monitoring of the bridge
對于大型重要橋梁來說，無論是對其進行復雜響應分析（如地震響應分析、抗風穩定性分析、車橋振動等方面的動力分析） ，還是進行長期監測和健康狀態評估，一個準確和有效的「基準」 （ baseline ）有限元模型都是不可缺少的前提。 -
Pebe2 vector containing the target gene and 35sih3 vector containing bar gene were co - bombarded into several indica rice lines shuhui527, minghui86 and 612s etc. resistant seedling were obtained finally. in order to establish rice mutation library, agrobacteriwn vector pshk22 containing bar gene as screening maker was transformed iniojaponica zhonghual 1
在水稻的遺傳轉化過程中，通過基因槍法將含有目的基因sbe2b的質粒載體pebe2和含有篩選標記基因bar的質粒載體35sih3共轉化到蜀恢527 、明恢86 、 612s等秈稻品種中去。
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