restriction fragment 中文意思是什麼

restriction fragment 解釋
限制酶斷片
  • restriction : n. 1. 限制，限定。2. 拘束，束縛；自製。3. 【邏輯學】限定。
  • fragment : n. 1. 碎屑，碎片，破片，斷片。2. 未完稿，斷簡殘篇。vi. ,vt. （使）成碎片，（使）分裂。
  1. It was suggested that eric - pcr could substitute for rapd in research related to the genetic identification and genetic diversity in auricularia and other edible and medicinal fungi : 2 to a certain extent, genetic differences among auricularia strains tested in this study did not have necessary relativity with their geographical origins respectively ; 3 in this study, genetic diversity in a. polytricha was higher than that in a. auricula : 4 in this study, a. fuscosuccinea had a higher homology to a. auricula than to a. polytricha ; 5 morphological characteristics validated the results from eric - pcr and provided a potential explanation for the higher similarity coefficient between a. auricular and a. fuscosuccinea ; 6 southern hybridization was employed by choosing a strain from a. auricula as a probe which hybridized with a. auricula and a. fuscosuccinea except a. polytricha, further confirming the veracity of the results from eric - pcr ; 7 in this study, isozyme analysis could not cluster the 7 strains from three auricularia species to different groups efficiently ; 8 2 strains from two auricularia species revealed high conservative degree and the restriction fragment patterns by 4 kinds of restricted enzymes showed no diversity

    本研究中，木耳屬2個種的2個菌株在its區域表現出較高的保守性， 4種限制型內切酶的酶切圖譜沒有顯示出多態性；增加內切酶種類及供試菌株數量，有可能獲得具有多態性的限制性內切酶酶切圖譜； 9本實驗中， its區域的真菌特異性引物與真核生物通用引物對于擴增效果無較大差異，擴增片段長度均為650bp左右； 10根據形態學實驗、 eric - pcr實驗以及southern雜交實驗的結果分析，紫木木耳屬種質資源的遺傳鑒定和遺傳多樣性評價耳極有可能是毛木耳種的一個變種; n .本研究中所用的gutc法是一種適用於木耳屬菌株基因組洲a快速提取的方法; 12 .傳統的形態學分類法和現代的分子生物學分類法，兩者的關系是相輔相成，互為驗證
  2. A double - strand cdna fragment of dh ( diapause hormone ) gene was obtained from kt - pcr amplification. the fragment was digested by two restriction enzyme nde 1 / ecor 1 and then was joined with pet - 30a ( + ) plasmid which was digested by nde 1 / ecor 1 too. then the outcome was transformed into escherichia coli bl21

    Pcr產物經nde / ecor雙酶切后，與同樣雙酶切的pet - 30a （ + ）質粒連接並轉化至大腸桿菌（ escherichiacoli ） bl21中，經檢測篩選，成功得到陽性克隆。
  3. Mouse ; restriction fragment length polymorphism ; dna fingerprint ; probe jl - 02 ; es cell

    小鼠限制性片段長度多態性dna指紋圖jl - 02探針胚胎幹細胞
  4. 1. because the taxonomic division is rather complex and has been much disputed and revised, in this part, we will review the classification and phylogeny of families, subfamilies and tribes of anseriformes based on morphology, ethology, osteology, mitochondrial and nuclear dna restriction fragment length polymorphism, single - copy nuclear dna hybridization and the sequences of mitochondrial gene analysis referring to the different definition, classification and phylogenetic relationships of the families, subfamilies and tribes of anseriformes. the controversial questions and deficiency in the systematic studies of anseriformes were pointed out

    具體包括以下幾個部分： 1 、針對雁形目鳥類異常復雜的分類狀況及分類上存在的爭議，根據雁形目鳥類的形態學、行為學、骨骼學、角蛋白、線粒體與核dna酶切片段長度多態、單拷貝核dna - dna雜交及線粒體基因dna序列分析等方面的研究，對雁形目鳥類分類中科、亞科和族的劃分及其相互間的系統發生關系進行綜述，分析系統學研究中存在的不足，提出了雁形目鳥類分類中急需解決的問題。
  5. Restriction fragment length pol - 2morphism, rflp

    因而有可能用限制性片段長度多態性
  6. Polymerase chain reaction - restriction fragment length polymorphism, pcr - rflp

    限制性片段長度多態性
  7. Restriction fragment lenth polymorphism ( rflp ) analysis n random amplified polymorphic dna analysis [ rapd ], the sequence analysis of internal transcribed spacers ( its ) of ribosomal dna ( rdna ), induction of microcyclic conidiation, sem ( scanning electron microscopy ) ascosporal isolation and other methods were applied to study more than 100 specimens or isolates of cordyceps, its anamorphs and other entomogenous fungi

    本文採用了rapd 、 rdnaits的rflp 、 rdna的its序列分析、誘發微循環產孢、掃描電鏡觀察、子囊孢子分離等方法研究了蟲草及其他蟲生真菌的100多個標本或菌株。
  8. Restriction fragment length polymorphism. pcr - rflp

    限制性片段長度多態性
  9. Objective through measure telomere length ( mean length of telomere restriction fragment, trf ) of dermal and intramuscular, and study it ' s length correlating with the different human " s age. the trf was examined by southern blotting. the formula to age estimating was obtained by regression analysis between the trf and the age

    目的應用southern雜交技術，對皮膚、肌肉端粒dna片斷長度（ meanlengthoftelomererestrictionfragment ，簡稱： trf ）進行觀察，測定不同年齡段人群的端粒dna片斷長度值，以期初步探明不同年齡段人群的端粒dna片斷長度的變化規律，繪制出端粒dna片斷長度值隨年齡變化的標準曲線，比較性別、籍貫對端粒dna片斷長度的影響，以期為法醫實踐工作中對無名屍體年齡推斷提供理論依據。
  10. Analyse the research of distributing of microbe community and the tendency of the change, disscuss the principle and traits of denaturing gradient gel electrophoresisand terminal restriction fragment length polymorphism, to research the law of change that the microbe community have in composting process, we can get effective and rapid information to filtrate the microorganism during composting process, then accelerate the development of compost technology

    摘要對堆肥微生物種群分佈及其動態變化的研究進行了分析，論述了分子生物技術中的變性梯度凝膠電泳和末端標記限制性片段長度多態性的原理和特點，以及用於研究堆肥微生物的群落結構演變規律，為分析和篩選堆肥中的微生物提供更加有效、快速的信息，促進堆肥技術的發展。
  11. Hbv genotype was determined by the restriction fragment length polymorphism analysis in patients with chronic hbv infection in 5 cities of fujian province. 2. the sensitivities and specialties of melting curve assay and pcr microplate hybridization - elisa assay were compared with mpcr - rflp and sequence analysis for the detection of hbv ymdd mutants in 44 serums from patients receiving lamivudine monitherapy with viral breakthrough

    應用熔解曲線法和pcr微板核酸雜交- elisa法對44例接受拉米夫定治療過程中出現病毒學反跳時的血清進行ymdd突變株的檢測，並與測序法和mpcr - rflp法比較它們的敏感性、一致性。
  12. Errors intrinsic to the testing systems, such as the inability to precisely measure dna restriction fragment lengths, are well compensated for by interpretation guidelines which take these kinds of errors into account

    鑒定系統本身固有的誤差，如不能精確的測量dna限制酵素片段長度，可以由解釋規則來很好的彌補，因為解釋規則會考慮到這些種類的誤差。
  13. The antigenic and genetic variability of porcine reproductive and respirators syndrome virus ( prrsv ) isolates in china were studied by immunofluoresent monolayer assays ( 1fma ) and restriction fragment length polymorphism ( rflp ) of reverse transcription ( rt ) and polymerases chain reaction ( pcr ) amplified - prrsvorfs fragments among 8 chinese isolates

    本研究通過對豬繁殖與呼吸綜合征病毒（ prrsv ）國內分離毒株的gp3 、 gp5和n蛋白的抗原性比較及其orf5和orf7遺傳變異性分析，系統研究了國內分離毒株的抗原特性和遺傳學差異。
  14. According to the southern blot results, a 6. 5 kb bamh i restriction fragment containing the gene coding for hal c8 was cloned and sequenced

    Halcs的開放閱讀框為849個核昔酸，編碼283個氨基酸;它必須要剪切掉n端的197個氨基酸才能成為成熟的蛋白。
  15. The hinf i restriction enzyme digestion gave rise to restriction fragment length polymorphism ( rflp ) of the pcr products. when there were two dna fragments of 363bp and 305bp were produced from a pcr product, the strains were assumed to have a mutation at ser83, when three fragments of 363bp, 206bp and 99bp were produced, the strains were assumed to have no mutation at the 83or 116. the results indicated that certain mutation of ser 83 abolished a hinf i restriction enzyme site and may be detected as a rflp

    本研究重點分析了16株菌對氟喹諾酮類（ fqns ）藥物的耐藥性，結果表明： 16株菌對諾氟沙星、環丙沙星、沙拉沙星、單諾沙星和氧氟沙星等（ nor 、 cip 、 sar 、 dan 、 ofl ）的耐藥率在31 . 3 - 56 . 3之間。
  16. Polymerase chain reaction and restriction fragment length polymorphism, pcr - rflp

    限制性片段長度多態性
  17. 1983 restriction fragment length polymorphism ( rflp ) technique used in cancer research

    1983年，限製片段長度多態（ rflp ）技術在癌癥研究中應用
  18. Some characteristics of cold - active protease and chitinase were analyzed then. microbial 16s rdna ( ribosomal dna ) clone libraries of deep sea sediments were constructed and studied by pcr - rflp ( restriction fragment length polymorphism ) and phylogenetic analysis based on 16s rdna sequences. the microbial diversity and community structures of deep sea sediments collected from two different sea area including the west pacific " warm pool " and the east pacific " manganese nodule " area, as well as the interaction between microbial community and environment, were analyzed based on these studies

    通過構建沉積物中微生物16srdna克隆文庫，採用pcr - rflp分析、 dna - dna雜交、 16srdna序列測定以及系統發育分析的方法，研究了兩太平洋「暖池」區和東太平洋「結核」區兩個不同海區深海沉積物中的微生物多樣性和群落結構特徵及其與環境的相互關系，得到了一些與「暖池」區環境特點緊密相關的新發現，新認識。
  19. All the subjects were genotyped by pcr - rflp ( polymerase chain reaction - restriction fragment length polymorphism ) at polymorphic sac i site inside the exon 7 of the ahsg gene. this polymorphism involves a nucleotide substitution of c to g at the middle nucleotide of the codon at amino acid position 238 resulting in the replacement of threonine ( acc ) with serine ( agc )

    所有的樣本通過聚合酶鏈式反應?限制性片段長度多態性方法（ pcr - rflp ）對ahsg基因的第7個外顯子內的sac多態性位點進行基因分型，該多態性位點為238號氨基酸密碼子中間的堿基c到g的替換，使蘇氨酸（ thr ， acc ）變為絲氨酸（ ser ， agc ） 。
  20. Over 13 kb saci restriction fragment was cloned into pgem - 7zf ( + ), mapped for restriction endonuclease sites and an about 5. 0kb fragment was further subcloned and sequenced. through coding region specific primer, we amplied it ' s corresponding cdna, named st901. st901 is 2889bp long, contains 1447bp putative promoter region within 5 " upstream and three exons ( 475bp, 140bp, 39bp ) and two introns ( 472bp, 2s3bp ) in the coding region, encodes a hydrophilic protein of 217 amino acid residues with a molecular mass of 24kda

    以同源探針篩選馬鈴薯基因組文庫，得到四倍體馬鈴薯基因組dna ? st901 ，基因全長2889bp ，含3個外顯子（長度分別為475bp ， 140bp ， 39bp ）和2個內含子（長度分別為472bp ， 253bp ） ； 5 』端含有1447bp的啟動子區段，該區段具備一般啟動子的基本元件tatabox和caatbox ； 3 』非編碼區長63bp ，具hind酶切位點，沒有發現保守的加尾信號。
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