restriction mutant 中文意思是什麼
restriction mutant
解釋
限制缺陷型- restriction : n. 1. 限制，限定。2. 拘束，束縛；自製。3. 【邏輯學】限定。
- mutant : adj. 【生物學】變異的；變異所引起的；與突變[變種]有關的,經過突變[變種]的。n. 突變[變種]型生物；突變。
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Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting
本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。 -
To construct eukaryotic expression vector of mbl gene with codon 54 point mutation, the target sequence in pgem - mbld plasmid, which conains mbl cdna with codon 54 mutant allele, was amplified by pcr. after the cdna fragement and plasmids pci - neo were prepared by digestion with sma i and sal i, the fragment was inserted into sma i and sal i site in pci - neo eukaryotic expression vector, and the recombinant vector, named pci - mbl54, was obtained. the pci - mbl54, digested with restriction enzymes, was found to contain the point mutation mbl cdna by agarose gel electrophoresis analysis
本實驗以ggc54gacmbl突變為研究對象，選用真核表達質粒pci - neo ，根據已構建好的含54位密碼子突變型mbl基因t載體的結構，設計合成新的引物， pcr擴增54位密碼突變型mbl基因，凝膠回收，雙酶切pcr產物和pci - neo質粒， t4連接酶連接，將前者克隆至後者的sma和sal位點，轉化大腸桿菌xl - 1blue ，氨芐選擇培養。 -
The restriction map of phzl318 carrying the entire add gene cluster from s. avermitilis nrrl8165 was made. its two subclones phz2104 and phz2105 were introduced into s. lividans mutant zx1
製作了攜帶完整add基因簇的phz1318的限制內切酶圖譜，根據酶譜進行亞克隆得到phz2104和phz2105 。
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