reverse cell 中文意思是什麼
reverse cell
解釋
逆環流圈-
Analysis of other - cell interference in the reverse link of vsg - cdma cellular system
蜂窩系統反向鏈路的他區干擾分析 -
A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv
以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。 -
Telomerase reverse transcriptase and nuclear factor kappa b p65 expression in human laryngeal squamous cell carcinoma
65在喉鱗狀細胞癌中的表達 -
An infectious eiav clone was recovered by transfecting fatal donkey dermal ( fdd ) cell cultures and donkey leukocyte ( dl ) culture in vitro with the full - length gene clone of dv. the virus ( designed pd70344v ) derived from the third passage in dl culture was observed by electron microscope and the reverse transcriptase ( rt ) activity was determined
將包含全基因片段的三個基因克隆以限制性內切酶消化后順次連接克隆到載體ptz18r上，構建了4個全長基因的分子克隆，分別命名為pd30343 、 pd70333 、 pd70343 、 pd70344 ，其中兩個轉移到低拷貝載體plg338上，命名為plgd30343 、 plgd70344 。 -
The eiav - pok8. 2 - his was transfected into the donkey leukocyte culture. following an incubation period, reverse transcriptase activity was detected in cell culture supernatants. cytopathic effect was observed by no. six passages post - infection in donkey leukocyte infected by the virus derived from eiav molecular clones eiav - pok8. 2 - his
提取前病毒dna ， pcr擴增p1p4片段，亞克隆至pmd18 - t載體，測序證明前病毒dna含有六個組氨酸，從而在體外獲得了感染性克隆病毒株。 -
The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins
將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。 -
Benzyl chloride were used for extracting genomic dna of aspergillus. niger 14, about 1. 5kb specific fragment was obtained from genomic dna of aspergillus. niger 14 by pcr amplification with primers ( forward primer5 " ataggcatcatgggcgtctct3 " reverse - primer5 " cagctaagcaaaacactccgc 3 designed according to the known sequences of the phytase gene in the gene bank and pyrobest ? dna polymerase, after ligated with pmd18 - tvector, transformated into e. colidh5a competent cell successfully. 3. nucleotide sequence analysis of the cloned fragment revealed the presence of the whole phya gene in pcr product
用氯化芐法提取了aspergillus . niger14 ~ #基因組dna ，根據genebank中已知的黑麴黴植酸酶基因序列設計出一對特異性引物（上游引物： 5 ataggcatcatgggcgtctc3下游引物： 5 cagctaagcaaaacactccgc3 ） ，採用pyrobest ~ （ tm ） dnapolymerase （高保真dna聚合酶） ，通過pcr方法從aspergillus . niger14 ~ #基因組dna中擴增出了預期的1 . 5kb左右的特異性產物，將其與pmd18 - tvector連接后，轉化e . colidh5菌株的感受態細胞，經質粒抽提、酶切鑒定，確認該目的產物已得到成功克隆。 -
A new finite - difference method is developed for seismic modeling and reverse - time migration based upon the voronoi cell construction on irregular grids. curved velocity boundaries and irregular earth surface can be accurately represented using the irregular grid implementation. therefore the modeled and migrated results can be more accurate, and meanwhile, the efficiency is still preserved using an explicit finite - difference scheme
本文的方法可以實現任意非規則網格上的顯式差分計算，因此該方法在保持了傳統的矩形網格差分演算法簡單、高效特點的同時，又能精確地描述彎曲界面和起伏地表，提高了有限差分地震模擬和偏移結果的精度。 -
Study of tea polyphenol on the reverse of carcinoma cell lines multidrug resistance
茶多酚對腫瘤細胞多藥耐藥性逆轉作用的研究 -
According to the described sequence of chicken interleukin - 1 ( chil - 1 ) and by using the extracted total rna of chicken peripheral blood mononuclear cell ( pmbc ), a pair of primers were designed to reverse transcriptionally amplify and to compare the expressional degrees of chil - 1 mrna by stimulating chicken pmbc for 4, 8, 12, 24 hour with lipopolysaccharide ( lps ) and for 24 hour with lps and actidione respectively. the results showed that stimulating chicken pmbc with lps and actidione to produce chil - 1 was a better way of artifical stimulation
根據國外報道的雞il ? 1序列，設計引物，以提取的雞外周血單核細胞總rna為模板， rt ? pcr擴增並比較了經lps單獨刺激雞外周血單核細胞4h 、 8h 、 12h 、 24h和lps與放線菌酮聯合刺激24h時il - 1 mrna表達水平，發現lps與放線菌酮聯合刺激雞外周血單核細胞是一種較佳的實驗方案。 -
Through reverse adsorption and elution, sam could be separated from the cell extract
Sam在酸性條件下帶正電荷，能與陽離子交換樹脂進行離子交換。 -
We detected the transcription and translation change of different thrs genes by semiquantitative reverse transcription - polymerse chain reaction ( rt - pcr ) analysisjelisa respectively. at same time the differentiated cell were identified by immunocytochemistry method. results ( l ) nsc induced by mitogens were nestin - positive and incorporated by brdu
以pdgf 、 t _ 3對nsc進行誘導分化，並分別用rt - pcr半定量法、 elisa法檢測nsc在不同誘導因子作用下分化前後thr各亞型在轉錄與翻譯水平上的表達變化，用免疫細胞化學方法鑒定分化后的細胞類型。 -
Interferon - is an antiviral cytokine produced by activated t cell and nk cell. it upregulated mhc ii antigen expression, influenced the cellular and humoral immune response and has pleiotropic regulatory effects on immune system. one gene was amplified by reverse transcription - polymerase chain reaction ( rt - pcr ) in splenocytes which was stimulated with cona for 24 hours
-干擾素（ interferon - ， ifn - ）主要由活化的t細胞及nk細胞產生，具有抗病毒作用，也具有上調mhc （ majorhistocompatibilitycomplex ， mhc ）類抗原分子的表達等作用，是影響機體細胞免疫和體液免疫反應的一類具有多種調節效應的細胞因子。 -
Expression of human telomerase reverse transcriptase and proliferating cell nuclear antigen in pleural and peritoneal effusion
在良惡性胸腹水細胞中的表達及臨床意義 -
Telomerase is a celler reverse transcriptase which catalyzes the synthesis and extension of telomeric dna. telomerase activation is not observed in normal tissues of somatic origin but in almost 90 % of human cancer, with the result the telomere shorten progressively with cell division and thus is a critical step for multistep carcinogenesis
正常人體細胞（非生殖細胞）中端粒酶活性的表達很低，或用目前的方法幾乎檢測不到，而在90以上的惡性腫瘤中可檢測到端粒酶活性，相應的良性腫瘤則為陰性或僅有少數陽性。 -
Effect of antisense human telomerase reverse transcriptase transfection on the growth of human gastric cancer cell lines
反義人端粒酶逆轉錄酶基因轉染對人胃癌細胞系的影響
分享友人