reverse transcriptase pcr 中文意思是什麼

reverse transcriptase pcr 解釋
逆轉錄酶pcr
  • reverse : vt 1 使顛倒，使倒轉，使反轉；使翻轉；翻（案）。2 掉換，交換。3 使成正相反的東西，完全改變。4 【機...
  • transcriptase : 轉錄酶
  • pcr : PCR ＝polymerase chain reaction 【生物化學】聚合酶聯鎖反應〈此項技術可用以復制犯罪現場發現的DNA小樣，從而有助於破案〉。
  1. In this study five recombinant plasmids containing hn ( hemagglutinin - neuraminidase ) genes of ndv ( newcastle disease virus ) were constructed, hn genes were amplified by reverse transcriptase - polymerase chain reaction ( rt - pcr ) and were inserted directly into eukaryotic expression plasmid pcdna3

    應用反轉錄-聚合酶鏈反應（ rt - pcr ）獲得兩株新城疫病毒（ ndv ）血凝素-神經氨酸酶（ hn ）基因cdna ，然後定向插入真核表達質粒pcdna3中，構建了含hn基因的重組質粒。
  2. G gene of rabies virus m, the of two main regions ( about 1000nt ), ranging from 3161nt to 4162nt and ranging from 4012nt to 4863nt of glycoprotein gene of rabies virus strain m, isolated from mouse in he nan, china were amplified by reverse transcriptase - polynerase chain reaction ( rt - pcr ) in order to complete glycoprotein gene of strain m. these regions were sequenced by the produce of pcr directly. comparison and analysis of nucleotide sequence and amino acid sequence deduced with that of other strains published was performed by computer with dnasisv 2. 5demo software

    本研究對我國河南某地野鼠體內分離的狂犬病野毒株mrv基因的3161位? 4162位（ 1001個堿基）和4012位? 4863位（ 851個堿基）片段進行了反轉錄pcr擴增和序列測定，得到mrv的糖蛋白基因全序列，用dnasisv2 . 5demo分析軟體，與已發表的代表性毒株g基因全序列進行核苷酸和氨基酸序列的比較分析，結果表明在同一基因型中， mrv和國際標準攻毒株cvs的同源性最高（ 96 . 5 ） ，和中國減毒株ctn的同源性最低（ 79 . 8 ） 。
  3. In this study, the whole e2 genes of two strains of classical swine fever virus ( csfv ) isolated from guangxi ( gx ) were amplified by reverse transcriptase polymerse chain reaction ( rt - pcr ) method and seqenced. the e2 gene fragments of csfv were 1090 base pair in length and encoded 364 amino acid residues

    研究採用反轉錄?聚合酶鏈式反應（ rt ? pcr ）技術對兩株分別從柳州（ gxlz ）和南寧（ gxnn ）分離的廣西流行豬瘟病毒（ classicalswinefevervirus ， csfv ）進行e _ 2全基因的擴增、克隆和測序。擴增片段長度為1090bp ，編碼364個氨基酸殘基。
  4. A reverse - transcriptase polymerase chain reaction ( rt - pcr ) based technique was developed to detect newcastle disease virus ( ndv ) of different poultry species origin. four oligonucleotide primers, based on the differences of nucleotide sequence at the cleavage site of fusion ( f ) protein gene between virulent and non - virulent strains of apmv - 1, were designed to amplify specific dna fragment from different viruses

    依據apmv - 1融合蛋白（ f ）基因裂解位點的核苷酸序列與其毒力的相關規律，分別設計合成了四條寡核苷酸引物，建立了一個可迅速檢測不同禽源apmv - 1並可鑒定強、弱毒株的逆轉錄酶?聚合酶鏈式反應（ rt - pcr ）技術。
  5. Nasopharyngeal aspirates swabs taken from the nose and throat swabs taken from these persons showed negative results for h5 avian influenza using rt - pcr reverse - transcriptase polymerase chain reaction by the public health laboratory centre

    公共生檢測中心對三人的咽喉分泌物所進行的快速測試反轉錄-聚合-鏈反應顯示，樣板對h5n1流感病毒呈陰性反應。
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