reverse transcription 中文意思是什麼

reverse transcription 解釋
反向轉錄
  • reverse : vt 1 使顛倒，使倒轉，使反轉；使翻轉；翻（案）。2 掉換，交換。3 使成正相反的東西，完全改變。4 【機...
  • transcription : n 謄寫，抄寫；轉錄；抄本，繕本，副本；轉寫，翻譯；【音樂】樂曲改作；【無線電】錄音；錄音廣播；（...
  1. Viral rnas were extracted from virus - infected allantoic fluids using qiaamp mini - extraction kits. after reverse transcription, cdna was amplified using specific primers for each gene segment

    用qiagen的rneasyrna試劑盒提取病毒rna后，逆助興才李醫『筍脂碩聖『老『才轉錄合成cdna 。
  2. The insulin - like growth factor - 2 ( igf - 2 ) and igf conjugated protein - 6 ( igfbp - 6 ) mrna level in rat calvaria bone tissue and mc - 3t3 - el cells were detected by northern blotting analyses and reverse transcription polymerase chain reaction. the estrogen responsive element ( ere ) in igfbp - 6 gene promoter was identified and involved in tcdd - reduced regulation of the gene expression by electromobility shift assays ( emsa )

    正常胎鼠頭蓋骨組織igf一2mrna呈高水平表達狀態，而igfbp一6mrna的水平較低; ccf胎鼠頭蓋骨骨組織內igf一2mrna的表達較正常胎鼠降低， igfbp一6mrna的表達則明顯升高; atra和e :聯合應用時， atra可以抑制雌激素對細胞內igf一2和igfbp一6的這種調節作用。
  3. A pair of primers were chemically synthesized based on the cdna sequence of hog cholera virus strain brescia and used to amplify the cdna fragments of envelope glycoprotein e2 gene of hclv which coding the major protective antigen by reverse transcription - polymerase chain reaction ( rt - pcr ) from total intact rna extracted from infected calf testicle cell culture by hclv

    以豬瘟兔化弱毒犢牛睪丸細胞毒（ hclv ）中提取的細胞總rna為模板，以rt - pcr技術，擴增出了完整e2基因的cdna片段。經電泳、酶切分析，證實了所擴增片段為e2基因特異性片段。
  4. The nucleoprotein ( n ) gene of three strains and glycoprotein ( g ) gene of 119, gxbm were amplified by reverse transcription - polymerase chain reaction ( rt - pcr ), respectively

    對三株的n基因和119 、 gxbm株的g基因進行了rt - pcr擴增、克隆和測序。
  5. Described here is the cloning and characterization of a new trichome - specific - promoter in arabidopsis. by ddrt ( differential display reverse transcription ) - pcr and reverse northern, a 280bp sequence is acquired from the epidermal in viciafaba, which is specially expressed in the epidermal. then using race, we abtain a 3. 0 kb gene segment including the 3 ' end full sequence and the 5 ' end partial sequence of the 280bp segment

    首先，我們利用差異顯示技術和反northern技術，以蠶豆葉肉細胞為對照，從蠶豆葉片表皮細胞中克隆出長280bp的新的表皮特異表達基因片段，再通過race技術獲得此基因片段的3 』端全序列和5 』端部分序列。
  6. Then cdnas were labeled with cy5 or cy3 respectively by incorporating cy5 - dctp or cy3 - dctp into the reaction during reverse transcription of rna

    採用cartesian基因晶元列印儀，將純化的cdna片段列印在corning玻片表面，製作了酵母細胞基因表達譜晶元。
  7. Total cellular rna were extracted, 40 micrograms of the total rna were used in the reverse transcription reaction, using superscript ii reverse transcriptase, oligo ( dt ) i8 primers, and cy3 - dctp or cy5 - dctp for the experimental and control group respectively. the labeled cdnas were hybridized to microarrays at 42c for 12 h - 18 h

    提取as _ 2o _ 3作用k562細胞前後的總rna ，用superscript逆轉錄酶逆轉錄成cdna第一鏈，並在逆轉錄的過程中，用cy3 cy5熒光染料分別標記對照組處理組，與自製的k562細胞基因表達譜晶元雜交。
  8. Then the plasmid was extracted from them and determined by dna calculator. the protoplast that contain growth hormone releasing factor injected into rabbit muscle and mouse muscle after it were treated by 1 % glutaral, pay it to electric stimulation in muscle of injection site and extract omni - rna from injection site of rabbit muscle, expression of grf were detected by reverse transcription and pcr ; ratio of expression of grf were detected by elisa. extract dna form injection site of mouse muscle to research time of expression

    本實驗是將含grf重組質粒的jm109菌株大量培養，用堿裂變法提取質粒，用dnacaculator定量；制備含grf的原生質體，經1的戊二醛處理后注射於家兔肌肉，在注射部位給予電刺激，提取總rna ，用rt - pcr檢測grf在肌肉中的表達；用elisa法定性檢測grf在肌肉中蛋白質水平的表達；將該原生質體注射于小鼠肌肉中，定期提取dna ，初步探討原生質介導的外源性grf在小鼠肌肉中的表達時間。
  9. Total rna was extracted from the second stage larve of hypoderma sp, then single chain cdna was synthesized by reverse transcription using oligo ( dt ) 18 as a primer. the hypodermin c ( hc ) and hypodermin a ( ha ) gene specific primers were devised by dnastar software

    本試驗的目的旨在進行hypodeminc （ hc ）和hypodermina （ ha ）基因的克隆、測序、構建重組表達載體並誘導表達，獲得重組抗原，以解決天然抗原的不足並為診斷和免疫試劑的產業化奠定基礎。
  10. Pgem - t vector system, jm109 competent cells, reverse transcription kit and in vitro translation kit were purchased from promega company

    Pgem一t載體，感受態細菌jm109 ，反轉錄試劑盒和體外翻譯試劑盒均購自promega公司。
  11. Protocol of reverse transcription polymerase chain reaction rt - pcr for infectious haematopoietic necrosis virus

    傳染性造血器官壞死病毒逆轉錄聚合酶鏈式反應操作規程
  12. To further confirm the expression levels of those differentially expressed genes in rat brain of + gz exposure group, the primers for two known genes ( 53 # and 72 # ) and one unknown genes ( 58 # ) were designed for semi - quantitative reverse transcription polymerase chain reation ( rt - pcr ), using gapdh as the reference

    4 、進一步驗證了通過ssh方法得到的差異表達基因片段在+ 1ogz重復暴露腦組織中的差異表達情況。對兩個己知基因克隆53 # 、 72 #及一個新基因克隆58 #分別設計引物，以gapdh為內參照，進行半定量rl 、 pcr反應。
  13. The centre for health protection of the department of health today november 18 received a report from the university of hong kong that laboratory tests using reverse transcription polymerase chain reaction rt - pcr method on three out of the seven specimens taken from patients of the caritas medical centre outbreak yielded positive results to parainfluenza virus, but all the seven specimens were negative for other common respiratory pathogens

    生署生防護中心今日十一月十八日收到香港大學的報告，明愛醫院病童的七個樣本經大學完成反轉錄-聚合鏈反應rt - pcr測試后，其中三個樣本證實對副流感病毒呈陽性反應，但是所有七個樣本均對其他常見的呼吸道感染病原體呈陰性反應。
  14. Firstly, the major ampullate glands were got from araneus ventricousus. total rna and mrna of ampullate glands were isolated and purified. double strands cdnas were synthesized in the assistance of amv - rt and e. coli dna polymerase i etc. by reverse transcription and replacement synthesis

    首先剝離大腹園蛛主壺腹腺，提取總rna和分離純化mrna ，反轉錄合成cdna ，凝膠層析除去小於400bp片段，並在雙鏈cdna兩端引入ecor的銜接頭，對其進行磷酸化處理。
  15. In this study, we designed a pair of primers based on the sequence of the upstream and downstream of chicken il - 2 gene. about 600 bp chicken il - 2 cdna fragment was cloned from cona - stimulated chicken splenocytes by reverse transcription - polymerase chain reaction ( rt - pcr ) and was subcloned into puc18 vector. recombinant clone was demonstrated by restriction enzyme digestion and dna sequencing. next, we construct recombinant plasmid pproex ? t - il - 2. the cdna of chicken il - 2 gene was subcloned into bamh i / hind iii sites of vector. the recombinant plasmid pproex ? t - il - 2 was transformed into e. coli dh5a and the bacteria was induced with iptg. it was demonstrated by sds - page and western blot that a 18kda protein which was equal to chicken il - 2 protein in molecular weight was expressed in e. coli dh5a. the expression level was up to 30 % of the total bacterial proteins. the purified protein was used to prepare the antibody against chicken il - 2 protein

    經酶切鑒定及dna序列測定，該基因為雞il - 2基因，其序列與sundick等報道的完全一致。在此基礎上，我們把雞il - 2基因亞克隆到大腸桿菌原核表達載體pproex ~ （ tm ） ht中，構建重組表達質粒並進行確證性序列測定，重組質粒測序結果表明將編碼雞il - 2成熟蛋白的基因正確地插入到原核表達載體pproex ~ （ tm ） ht的目的位點。重組質粒轉化受體菌dh5後用iptg於37進行誘導培養， sds - page和westernblot分析顯示，表達的雞il - 2融合蛋白分子量約為18kda ，表達的融合蛋白經薄層掃描發現目的蛋白表達量約占菌體蛋白的30 。
  16. The antigenic and genetic variability of porcine reproductive and respirators syndrome virus ( prrsv ) isolates in china were studied by immunofluoresent monolayer assays ( 1fma ) and restriction fragment length polymorphism ( rflp ) of reverse transcription ( rt ) and polymerases chain reaction ( pcr ) amplified - prrsvorfs fragments among 8 chinese isolates

    本研究通過對豬繁殖與呼吸綜合征病毒（ prrsv ）國內分離毒株的gp3 、 gp5和n蛋白的抗原性比較及其orf5和orf7遺傳變異性分析，系統研究了國內分離毒株的抗原特性和遺傳學差異。
  17. After one - step reverse transcription of the rna virus genomes, the viruses " specific dna fragments were amplified by nested three loci pcr, which the amplification system was optimized by uniform design

    選擇合適的核酸抽提方法，一步法抽提hbv 、 hcv及hiv 1的病毒核酸並一步法逆轉錄病毒的rna ，獲得病毒的dna 。
  18. Positive probe was made from the floral meristem materials cultured for 5 days and 10 days with hormones by rna reverse transcription and labelling with radioactive dctp ; two negative probes from uncultured explants and it cultured for 5 days and 10 days. then, we did calculating signal arrays, sequencing, sequence analysis and alignments on genebank etc. finally 229 different ests were got from the cdna library

    用在含有外源激素的培養基上培養5天（花分生組織開始形成）和10天（花分生組織已基本形成）的風信子外植體為材料，構建起cdna文庫。利用cdnamicroarrray技術，經過雜交篩選和est分析序列分析，最終從cdna文庫中獲得了229個不同的外源激素誘導響應的基因序列。
  19. A pair of degenerate primers were designed in the conserved domain which based on the alignment of acbf gene family in tobacco and arabidopsis. a 239 bp fragment was amplified by rt - pcr ( reverse transcription polymerase chain reaction ), which was used as a probe for screening tomato fruit ( pink stage ) cdna library. one positive clone containing entire coding region were isolated, which was identified as a new member of acbf family by blast server, and named as leacbf

    根據genbank （中的擬南芥、煙草acbf家族成員序列比較的結果，在該基因的保守區設計簡並引物（ degenerateprimer ） ，以轉色期普通番茄果實的rna為模板，進行rt - pcr擴增，獲得239bp的擴增片段，以此片段作為探針篩選轉色期普通番茄果實cdna噬菌體文庫，獲得了包含全長編碼區的陽性克隆。
  20. The aim of this study was to investigate the differential expression of mpges and cpges in mouse uterus during early pregnancy and its regulation under different conditions by in situ hybridization and immunohistochemistry. mpges expression in the preimplantation mouse embryos was also performed by reverse transcription pcr ( rt - pcr )

    本實驗首先利用原位雜交和免疫細胞化學的方法，檢測小鼠正常妊娠1 - 8天子宮中mpges和cpges的表達情況，並利用假孕、延遲著床和人工誘導蛻膜化等模型研究mpges和cpges的表達及其調節。
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