rt pcr test 中文意思是什麼
rt pcr test
解釋
多聚梅反應測試-
The sucking mouse brain were inoculated with mdj - 01 strain to make electron microscopic examination, results showed that the virus was a spheral particle with membran which had a diameter of about 40 nm. by indirect fluorescent antibody test mdj - 01 strain was identified with tbev. a part of region encoding e protein was expanded by rt - pcr and sequenced. the nucleotide sequences of two strain viruses were compared with sequences in genbankjsequence homology analyses revealed mdj - 01 strain and senzhang strain had the highest homology with tbev oshima5 - 10, respectively, which were 95 %, 94 %. mdj - 01 strain was identified with tbev again
應用間接免疫熒光試驗進行血清學鑒定，結果表明mdj - 01株為tbev 。通過rt - pcr技術擴增部分e蛋白序列並測序，在genbank上進行同源性比較，發現mdj - 01株和森張株與tbevoshima5 - 10株的同源性最高，分別為94 、 95 ，從分子生物學水平上進一步證明mdj - 01株病毒為tbev 。在鑒定的基礎上，本實驗對兩株病毒進行了核苷酸全序列測定。 -
Transgenic plants were comfirmed by molecular determination including pcr, southern blot and rt - pcr. 4. functional analysis and iron content of transgenic plant crude proteins of transgenic plant were extracted from leaves, and then used to test its antibiotic activities
4 .轉基因植株抗菌能力加強，鐵含量得到提高提取轉基因植株葉片粗蛋白，以大腸桿菌dhsq和酵母菌ahiog為試驗靶標，側定了轉基因植株粗蛋白的抑菌活性。 -
In order to test the above working hypothesis, the contractile functions in unloaded soleus were observed by using in vitro skeletal muscle strip perfusion, the expression of mhc proteins and mrna was detected by sds - page and rt - pcr, respectively
為驗證這一設想，本研究採用大、小鼠尾部懸吊模型模擬失重狀態，用離體骨骼肌肌條灌流方法觀測收縮功能的變化，並採用sds - page與rt一pcr技術，觀測比目魚肌mhc異構體的表達。 -
In the laboratory experiment part, human peripheral blood, cultured cells and icr mice were study objects. the changes of mitotic chromosome numbers were measured by human metaphase chromosome counts and statistic analyzed used x2 - test. the changes of meiotic chromosome numbers were measured by mice one - cell zygote chromosome counts and statistic analyzed usedx2 - test. the effects of low dose ionizing radiation on the expression of topoisomerase ii were measured by immunocytochemistry, western blot and rt - pcr
流行病學結果顯示長期小劑量輻射接觸與染色體不分離呈正相關，為進一步在細胞遺傳學和分子生物學方面研究小劑量電離輻射與染色體不分離關系及其機制，本課題第二部分以外周血、培養細胞、 icr小鼠為研究對象，用外周血染色體計數和單細胞受精卵染色體計數的方法研究小劑量輻射和拓撲異構酶復旦大學2000級博士生學位論文11a抑制劑及其二者的協同效應對有絲分裂和減數分裂染色體不分離的影響，用免疫細胞化學染色、 westernblot 、 rt pcr等方法研究了電離輻射引起拓撲異構酶a表達變化。 -
Methode competence is induced in the s. pn detached from clinic and creat the come duplicaion mutants by insertion - duplication. use the real - time rt - pcr to test the expressing level of vurilence between the mutants and the wild type
8的ctm中生長的菌作為陰性對照。分別在生長的不同時間點提取rna ，最後採用rea卜timert ? pcr來定量測定各種毒力因子mrna表達的水平。 -
To develop a rapid diagnostic test based on serum antibody to sars virus. coupled with clinical observations, the antibody test and the rt - pcr test could help in making rapid diagnosis, and thus allowing effective treatment using ribavirin and steroids at an early stage
發展出一套sars抗體的快速血清測試方法，並配合臨床觀察，此抗體測試及逆轉錄-多聚酶測試（ rt - pcr ）能作出快速而準確的診斷，有助於發病初期使用利巴韋林及類固醇進行有效的治療；
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