sal 中文意思是什麼

Sal island is currently the main tourist destination in cape verde as it has the largest international airport in the country

薩爾島是維德角目前的主要旅遊目的地，薩爾島國際機場是維德角最大的機場。

The next day cherokee sal had such rude sepulture as roaring camp afforded.

第二天，切羅基人莎被草草地埋了，咆哮營能提供的不過如此。

In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。

Linearized the expression vector ppic9k - p including the truncated mutant pokeweed antiviral protein ( pap ) gene by restriction endonuclease sal i and transformed it electrically into pichia pastoris gs115. mut + recombinants were selected by pcr and high yield mut + recombinant was picked out by double film immunoblotting

本研究將含有n末端信號肽和c末端毒性區缺失的pap基因的表達載體ppic9k - p用sali酶切線性化后，通過電擊轉化整合p . pastorisgs115菌株細胞中。

Sandy jipton thought it was rough on sal.

桑迪蒂普頓覺得這可苦了莎兒。

The dye trypan blue has a remarkable biphasic effect on pinocytosis by yolk sal.

染料錐蟲藍對卵黃囊的胞飲作用具有明顯的雙向作用。

- sal ly, which one is it gonna be ? - handy ! handy

-薩莉，到底是誰? -漢迪!漢迪

Hey, sal. don ' t tell me you ' re pulling out too

嘿， sally ，別告訴我你也要退出

Crab, crab, crab. shot. throw it away. sal ute

螃蟹，螃蟹，螃蟹設計.扔掉.敬禮

Sal said : coach, because he wants to be chairman

薩爾說:教練，因為他要成為董事長

Study on the frequency stability problem of sal

合成孔徑激光雷達頻率穩定性問題研究

Sal : oh, yeah ? what were you saying, then

哦，是嗎？說我什麼來著呢，你們？

My last boyfriend sal junior, is a scum bag

我上一個男朋友小薩爾也是個混蛋

On a completely different note, japan s sal vanilla will combine dynamic mass dance with cutting - edge effects of digital technologies to create a milieu of surrealism in the hong kong cultural centre studio theatre

日本salvanilla以動感十足的群舞配合前衛數碼科技效果，將香港文化中心劇場轉化為超現實空間。

Sal asked the owner, " do you have any of those throwaway cameras ?

薩爾問店主： 「你們有那種用了就扔的照相機嗎？ 」

In thl1 gene, there are some famililar enzyme digest sites including bamh i, hind, sac i and sal i. the sequence of thl1l from brassica oleracea l. shows 99 % identity to the sequence of thll from brassica napus l. there are some phosphorylation sites and an active site of thoiredoxin h members consisting of cppc in thll protein

甘藍和油菜thl1基因序列有5個堿基的差異，而氨基酸僅有2個氨基酸的變異，同源性達99 。在thl1蛋白的氨基酸序列中發現了一個硫氧還蛋白家族的活性位點（ cppc ）和多個磷酸化位點。

The dna encoding the desired protein was generated as a bamh i - sal i fragment using pcr. the template was pbv220 - hgf - a strand that had been constructed in our lab

其中gst是一種純化標簽，可以在後續的蛋白分離純化中使用gst親和層析的辦法純化目的蛋白，這將使純化工作變得簡單而有效。

How to rocket lauh your backend sal o

如何在火箭發射你的後端銷售！

That ' s when danny went crazy, tried to knock over sal ' s

丹尼后來就捉狂，試著去闖空門

In the end, he came to a river and took a bath. then he made a rope bed among eight sal trees, with each direction has two. he lied on his side, right hand supporting his head, the other resting on his body

佛在毗舍離城的時候，已經有了重病，在那裡度過雨季，偕弟子們向西北走去，路上受了鐵匠純陀供獻的食品，病更加劇，最後走到拘屍那伽一條河邊，洗了澡，在一處四方各有兩棵娑羅樹的中間安置繩床，枕著右手側身臥著。