screened positive 中文意思是什麼
screened positive
解釋
加網陽片-
Detection of antigen - binding affinity of mg7 recombinant phage antibody elisa was repeated to confirm the antigen - binding affinity of positive clones screened out in the former procedure ; these positive phages were examined by restriction analysis ( ecor i and hind iii ) ; competitive elisa was performed to test the inhibitory ratio of these positive clones to the binding affinity of mg7mab and its relevant antigen, the positive dones possessing apparent inhibitory effect were singled out for later use
X陽性克到駛知烘扳原kbbjg ）濁對陽性克隆進行限制隴臥賜析（ uwi和hedlll ）鑒定三用競爭elisatoljmg7重組噬菌體抗體性克隆對mg7單扶與其相應抗原結合忙的喇率，從中j ） ed出對mg7單抗與期眩抗原結合有抑余j效應的克隆用於進一涉研究。 -
The positive colonies that grew on the ampicillin ( amp ) plate ( lb agar medium contaning 100 g / ml amp ) were screened and identified. sds - page and western blot analysis were performed to study the expression profiles of target gene cein in e. coli
從轉化的平板中篩選出陽性重組子，進行不同iptg濃度和不同誘導時間的表達研究，並利用sds - page電泳和westernblotting蛋白質印跡技術對外源基因cei _ （ 12 ）在大腸桿菌e -
Then the linked products were transformed into the high competent cell of e. coli dh5a. based on - complementation of the detective - galactosidase, positive recombinant clone were screened from x - gal plate
從引物和基因序列的比對分析結果看， zhyf006序列與上下游引物的配對比例分別為s4 -
One positive clone has been gained from the library screened by m4g3 mcab
3 .文庫擴增后滴度達到1 -
In the third experiment, we first constructed a test model of differentiation from mouse es cells to cardiomyocytes. then series of dilutions of three chemicals were screened on this platform. the combination of all trans retinoic acid and dmso is employed as positive control
實驗三首先建立了一個小鼠胚胎幹細胞定向分化成心肌細胞的實驗平臺，然後採用了3種心肌疾病相關的藥物：黃芑生脈飲、 1 ， 6二磷酸果糖和hg （代號） ，在此定向分化的實驗平臺上進行了一系列濃度的藥物篩選，實驗採用視黃酸和dmso的混合誘導劑作為陽性對照。 -
The interesting gene fragment with ecori and noti were amplified by overlapping pcr, which inserted into vector plasmid ppic9k after degisted by ecori and noti, and the recombinant plasmid was transformed into competent dh5cc. positive clones were screened by pcr from the lb plate with amp. digesting analysis resulte shows that the interesting gene were inserted into the vector ppic9k with correct direction
目的基因經雙酶切后連接載體ppic9k ，然後導入大腸桿菌dh5中，在含氨卞青霉素（ amp ）的lb板上用pcr反應篩選出陽性菌落，雙酶切結果表明目的基因已插入載體中，且方向正確，測序結果進一步證明人巨細胞病毒重組基因表達質粒成功地克隆了目的基因片段。 -
We screened the cdna expression library by using 12 b - deoxoartemisinyl - ( 4 ' - oxyacetic acid ) phenyl ether conjugated with bsa. one of the screened positive clones was inserted tctp of plasmodium falciparum from hainan. the tctp was cloned to express protein in order to provide materials for further study
把青篙素類衍生物: 12b一二氫青篙素（ 4 』一氧乙酸基）苯醚用bsa標記作為探針，對惡性瘧原蟲海南株cdna表達文庫篩選，分離出插入了惡性瘧原蟲海南株tctp基因的陽性單克隆。 -
The e2 gene was amplified by rt - pcr, then examined the fragment by electrophoresis. after purification and insertion into pucm - t vecter, the recombinant plasmid pbne2pi and pbne2pii were obtained. then they were transfected e. coli jm109 and screened positive clones by blue or white plaques. the recombinant plasmids were extracted and the inserted fragments were identificated by electrophoresis
通過rt - pcr擴增e _ 2基因，電泳測定擴增片段的大小，經純化后，連接于pucm - t載體，獲得重組質粒pbne2p 、 pbne2p ，轉化e ? colijm109 ，經藍白斑篩選，挑取陽性克隆，提取質粒，直接電泳鑒定和酶切鑒定。 -
The positive clone was screened and identified by pcr and analysis of restriction endonuclease digestion
轉化感受態大腸桿菌dh5 ，經pcr及限制性內切酶分析得到陽性克隆。 -
3. pbv220 - endostatin was transformed into e. coli dh5a. the positive colony was screened and identified by pcr and restriction endonuclease digestion
3 .將重組質粒轉化到克隆菌dh5a中，經pcr篩選和限制性內切酶鑒定，得到陽性克隆菌株。 -
The recombinant plasimid ppic9k - pzp3 a - hcg p - ctp109 - 145 was transformed into electroporated pichia pastoris and screened the positive strains on md plates without histidine. multi - copy recombinants were screened on the g418 - ypd plates and then were incubated with the supernatant containing methanol. the recombinant pzp3 a - hcg p - ctp 109 - 145 protein were secreted in the supern atant and were verified
轉化嗜甲醇酵母獲得耐受4mg mlg418的菌株，經sds - page電泳和westernblotting檢測表明其上清培養液中的蛋白可與猴抗pzp3抗體和兔抗hcg抗體發生特異性的反應。 -
The objective of this research is to transform the cloned phytase gene into pichia pastoris in order to obtain high - yield phytase - producing strain and to optimize the engineered yeast media recipes for the scale - up production of phytase. main results of this research are as follow : 1, xba i - linized recombinant plasmid ppic9k - phya was transformed into pichia pastoris by gene pulser. 98 positive transformants showing measurable phytase activities were screened on md plates and ypd plates containing g418. they all grew quickly on both md plates and mm plates, which proved their phenotypes of methanol utilization were mut +
主要實驗結查如下：西南農業大學碩士學位論文1 、用乃ai酶切攜帶植酸酶基因表達片段的重組質粒ppicgk夕句） a ，回收dna ，用genepulser電擊轉化畢赤酵母，塗布md平板，又用含不同濃度g418的ypd平板進行抗性篩選，得到98個可檢測到植酸酶活力的陽性轉化子，它們在md 、 mm平板上均表現快速生長，說明其甲醇利用表型是mut干。 -
These results suggest that kyot plays an important role in the development of testis and spermatogenesis. 2 we performed yeast two - hybrid screening of a cdna library from human lymph nodes using kyot2 as a bait protein. 42 clones were gotten after 5xl06 were screened by four kinds of nutrition limitation and p - galactosidase assay, 22 clones were got after restriction of positive clones, at last, 13 genes were got by sequence assay including rbp - j known as the interacting protein with kyot before and two novel genes
對此22個克隆進行序列分析，共獲得13種基因，其中包括已知的kyot相互作用蛋白rbpj和兩個未知基因，也包括在哺乳動物睪丸中特異3第四軍醫大學博士學位論文性表達的蛋白piasi ，同時還篩到了具有可與lm結構鞠互作用的pdzdomain分子緊密連接蛋白2和兩種同屬于kg家族的蛋白ringi和polycomb2 -
After the pbd i and pbd ii gene ligated with the expression vector pinpoint ? a - 3, the recombinated plasmid ppd - 1 and ppd - 2 was transformed into jm109 strains, 4 positive clones were screened by restriction enzyme analysis, dna sequencing showed that two out of 4 positive clones inserted sequence of the constructed plasmid, which was the same as that of pbd - i and pbd - ii gene respectively, and its reading frame was correct, thus its could be used to express fusion protein
將pbd - 、 pbd -基因與表達載體pinpoint ~ （ tm ） xa - 3連結后獲得的重組質粒ppd - 1 、 ppd - 2轉化于大腸桿菌jm109中。經抽提質粒、酶切分析及pcr擴增，分別篩選到4個陽性克隆，將其中二個陽性克隆由測序分析，證實1個含pbd i基因片斷， 1個含pbd 11基因片斷，且閱讀框正確，可用於融合蛋白表達。 -
The ubi - sl - tocs fragment was taken out, inserted into the multi - cloning site of pcambia1300 vector, transformed into jm109 strain finally, positive colonies were screened on lb plate ( 60 g / ml kan added ). the result of pcr and enzyme digestion of plasmid proved that recombinatin vector was obtained ( named pcusaib4 and pcusaibu )
把擴增產物分別通過clai和bamhi酶切純化，連接到用clai和bamhi切去gfpml基因的中間表達載體pugfpocs中，轉化大腸桿菌jm109 ，在含amp （ 100 g / ml ）的lb抗性平板上篩選到了的陽性菌落。 -
2 specific epitope analysis in m3m4 loop target to determine the b cell epitope of a monoclonal antibody against the m3 - m4 loop of nmdar1, a random phage displayed dodecapeptide library was screened with the monoclonal antibody mab363 against the m3m4 loop of nmdar1. after four rounds of biopanning, the peptide sequences of positive phage clones were determined and analyzed by dna sequencing
流式細胞儀測定脾細胞cd4 + 、 cds寸淋巴細胞亞群，間接elisa測定血清thl細胞因子幾一2 、 tn下一a和n一y ， th一2細胞因子幾一4 、 il巧和幾一6的水平，同時檢測特異性抗體產生情況。 -
Blood samples from 12, 000 hong kong citizens were collected and screened for the presence of the sars - cov antibody using enzyme - linked immunosorbent assay ( elisa ). positive samples underwent immunoflourescence assay ( ifa ) and western blot for confirmation
經初步酵素連結免疫吸附分析法（ elisa ）測試呈陽性的樣本更會透過免疫螢光法分析法（ immunofluorescenceassay ） ，及西方點墨法（ westernblot ）化驗以作最終確定抗體的存在。 -
Putative transgenic plants were screened b y nptii - specific and mnsod - specific pcr amplification and southern blotting, 84 % of the transgenic plants gave positive results. the results of mnsod activities demonstrated that tobacco mnsod gene contributed to more than 50 % of the total mnsod activity in some transgenic alfalfa
Npt基因和mnsod基因的pcr檢測和southern雜交表明mnsod基因已經整合到84的保定苜蓿轉基因植株的基因組中， mnsod活性測定結果表明轉基因植株中的mnsod活性與對照植株之間存在顯著差異，部分轉基因植株的mnsod活性比對照植株提高了1倍以上。
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