screening sequence 中文意思是什麼
screening sequence
解釋
篩選程序-
Genetic diversity and phylogeny of 55 slow - growing rhizobia isolated from peanut ( arachis hypogaea ) in china were determined by analysis of host - plant range, phynotype, 16s rrna rflp, 16s rrna sequence, 16s - 23s igs rflp, rapd, rep - pcr, dna - dna hybridization homology. at the same time, the competitive nodulation capacity of rhizobia, effect of host plants and soil ph on the rhizobia were determined for screening and improvement of high effective rhizobium inoculant
本研究採用宿主范圍試驗、表型性狀測定、 16srrna - rflp 、 16srrna序列分析、 16s - 23srdnaigsrflp分析、 rapd分析、 rep - pcr分析和dna - dna同源性分析等技術系統研究了從我國不同地域分離的55株花生根瘤菌的遺傳多樣性及其在根瘤菌系統發育中的地位和相互關系。 -
By blue - white screening and sequence analysis, we obtained the positive clone. 2. we constructed plant binary expression vector carrying fusions of the enhanced camv 35s promoter and dreb1c ( 35s : dreb1c ) in the sense orientation
我們從擬南芥基因組中克隆了dreb1c基因全長，並將其連接到中間載體pgem - t - easy上，進行了測序驗證，結果顯示所克隆的dreb1c序列完全正確。 -
After screening, we had identified ten candidate clones which are differentially expressed at salt - treated leaves of aloe vera l. sequence analysis was performed for three clones. one of them scored perfect to already known nadp - malic enzyme gene
這些表明，鹽脅迫可以誘導這兩種蘆薈中的nadp一蘋果酸酶基因的表達和nadp一蘋果酸酶蛋白的積累，但是誘導的強度與蘆薈品種的耐鹽程度有關。 -
Acetylornithine deacetylase is the key enzyme of producting l - methionine. we mainly do research work on the construction of acetylornithine deacetylase gene - engineering strain and characteristic of proteinase. in order to get high expression deacetylase strain, we obtain the gene by pcr arge gene. the product ( 2800bp ) was cloned into puc19 plasmid and confirmed with blue / white dot screening > restriction enzyme analysis and pcr. then taking the nucleotide sequencing compared with the sequence at blast of u. s. a. we constructed a high expression of gene - engineering strain - pxj 128 which containing the arge gene on the high expressing system of pxji18 with activity of acetylornithine deacetylase above 20000u / g
為了獲得高效表達的脫乙酰鳥氨酸酶工程菌株，在工程菌技術改造及其固定化研究做了進一步的研究和探討。我們採用基因工程技術，通過pcr技術擴增出了酰化酶關鍵酶基因?脫乙酰鳥氨酸酶基因arge ，將其克隆到puc19載體中，經酶切鑒定、 pcr鑒定篩選出重組陽性質粒，並測序鑒定，通過美國blast程序進行了基因數據庫相似性比較分析。 -
Recently, two distinct genes, dreb1 and dreb2, encoding dna binding proteins that specifically bind to the dre sequence of rd29a gene had been cloned from arabidopsis by using the one - hybrid screening technique
它們編碼著與擬南芥rd29a基因的dre順式作用元件特異結合的轉錄因子，參與在乾旱，高鹽及低溫脅迫條件下的rd29a基因的表達調控作用。 -
In the past, we cloned the cdna of zmcdc5 based on the studies on difference of gene expression in shoot and radicle of maize seedling in our lab and then got the whole genome sequence by maize genomic library screening and inverse pcr
本實驗室根據玉米胚芽和胚根差異表達的研究，克隆得到zmcdc5基因的cdna ，並通過篩庫和反向pcr獲得了zmcdc5基因組全長序列。 -
It is true in difficult cases, such as degraded dna or samples containing minimal amounts of genomic dna. to investigate the sequence diversity of mtdna in taiyuan han population, mtdna nt16081 - 16546 sequences were determined in 58 unrelated han chinese from taiyuan. sscp method was developed as one of screening measures which were used to examine polymorphism of mtdna, so as to supply a rapid and simple method for forensic casework
方法隨機抽取58名太原地區漢族群體無血緣關系個體的靜脈血， edta抗凝，改良的tkm法提取mtdna ， pcr擴增、純化后，應用377序列分析儀進行直接測序分析；對毛發、指甲、骨骼等微量或嚴重降解檢材進行mtdna序列分析；採集同一屍體血液、肌肉、肝、腎、心肌、不同部位的毛乾等組織進行同一性檢測；對20個兩代家系進行突變觀察。 -
Part ii screening of tnfa mimotopes from phage display peptide library : based on the results of screening tnfa binding - peptides, we have tried to use neutral tnfa mcab j1d9 as target to screen tnfa mimotopes from c7c phage display peptide library, which may be another form of antagonist for tnfa, and the mimotopes were identified by sandwich elisa. after 3 rounds of screening, we got 9 phage clones identified as positive clones which can bind with mcab j1d9. we also identified the binding between mimotopes and tnf receptor by competitive elisa, and the results showed strongly binding. the amino acid sequence results shown three different sequences : c - rrpaqsg - c - nkhnrki - c and c - rgmsrki - c
在對噬菌體環七肽庫進行三輪親和性篩選后，隨機挑選20個噬菌體克隆， elisa鑒定出9個陽性克隆，經dna測序推出三種氨基酸序列： c - rrpaqsg - c 、 c - nkhnrki - c和c - rgmsrki - c ，其中優勢克隆序列為c - rrpaqsg - c ；鑒定結果顯示陽性克隆能夠與tnf受體結合，並且能夠阻斷tnf與受體的結合，提示篩選得到的環七肽克隆展示肽具有tnf的抗原性及與tnf受體結第一軍醫大學顧士學位論文合的特性，為tnfa表位模擬肽。 -
These results suggest that kyot plays an important role in the development of testis and spermatogenesis. 2 we performed yeast two - hybrid screening of a cdna library from human lymph nodes using kyot2 as a bait protein. 42 clones were gotten after 5xl06 were screened by four kinds of nutrition limitation and p - galactosidase assay, 22 clones were got after restriction of positive clones, at last, 13 genes were got by sequence assay including rbp - j known as the interacting protein with kyot before and two novel genes
對此22個克隆進行序列分析，共獲得13種基因，其中包括已知的kyot相互作用蛋白rbpj和兩個未知基因，也包括在哺乳動物睪丸中特異3第四軍醫大學博士學位論文性表達的蛋白piasi ，同時還篩到了具有可與lm結構鞠互作用的pdzdomain分子緊密連接蛋白2和兩種同屬于kg家族的蛋白ringi和polycomb2 -
After extending the sequenced region tb whole control region, the sequence in han, li, wei, yao, zang ethnic groups revealed that the discrimination power of mtdna was increased greatly. the novel str and snp loci found in the region of mtdna also provide useful markers for screening test of forensic samples
對漢族、黎族、維族、瑤族、藏族群體進行序列分析結果表明，將檢測區域從hvi和hvh擴大到覆蓋整個控制區及其周圍區域后大大提高了mtdna這一遺傳標記在法庭科學領域的鑒別能力。 -
Through three rounds of screening, seventeen clones were selected and used in competitive test. the mab ge3 could specifically inhibit eight out of seventeen clones from binding to swine antisera. based on the amin acid sequences deduced from the foreign sequence inserted in the phage, it was indicated that all clones shared the core sequence - p / ekphf, that was similar to aa50 ~ aa55 domain of n protein of prrsv
從第三輪親和篩選的噬菌體中隨機挑取17個克隆進行功能鑒定，結果表明8個克隆與mabge3具有較強的特異性結合力並可以被prrsv陽性血清阻斷，測序發現7個克隆具有核心序列： p ekphf ，該序列與prrsvn蛋白aa50 aa55 （ p ekphf ）具有較高的同源性。 -
After 3 rounds of screening, 10 of 20 clones were identified as positive clones and all the 10 phage clones sharing the same amino acid sequence : ehmaltypfrpp, and these positive 12mer phage clone peptide can bind with tnfa specifically. the results suggests that this method is very specific and simple for screening of binding epitope to small peptide molecule from phage display peptide library
在以噬菌體環七肽tnfa模擬肽克隆刁k naqsoc ）為靶對噬菌體隨機十二肽庫進行h輪篩選后，挑取20個克隆，經elisa鑒定有10個克隆均與t 』 nfa結合，測序結果顯示它們均為同一氨基酸序列： ehmaltypfrpp 。
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