sds 中文意思是什麼

The result of sds - page certificated the method 6 % octanoic acid combined with ammonium sulfate got the highest purity, the next was alginic acid sodium combined ammonium sulfate

Sds - page和低壓層析的結果證明，用6辛酸?硫酸銨法提取igy的純度最好，其次是海藻酸鈉?硫酸銨法。

Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions. so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor. in this study, radiolabelled ligand is used to investigate the binding characteristic of cam and a. thaliana protoplasts. and chemical crosslinking is employed to explore binding proteins in the membrane. at first, ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e. coli. sds - page and autoradiograph indicated high - purified, high - specific radioactivity ( 35 ) ~ s - cam was obtained. electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis

首先，用~ （ 35 ） s標記的氨基酸混合物喂養工程菌成功地制備了~ （ 35 ） s標記的擬南芥鈣調素（ ~ （ 35 ） s - cam ） ， ~ （ 35 ） s - cam純度高、放射活度高、 ca ~ （ 2 + ）與egta存在時的電泳行為與未標記cam相同，可作為一種高靈敏性的探針用於進行受體學分析實驗；用擬南芥種子誘導愈傷，通過酶解制備了大量原生質體。

Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity. so the bacteriocin is consisted of two proteins with different mw. only one protein with larger mw can be detected through tricine - sds - page, and its mw is about 8, 570da

採用30硫酸銨就能完全把發酵液中的細菌素全部沉澱，通過生物膠bio - gelp - 6層析發現細菌素被分離出兩條抗菌蛋白峰，這表明r21 - 4產生的細菌素是由兩種不同分子量的蛋白質組成的，通過tricine - sds - page檢測，只能檢測到一條分子量相對較大的細菌素，分子量在8 ， 570da左右。

Study on the washing activation energies of sds n - butanol decane water system

水微乳體系洗滌的研究

Fourthly, according to the activity, collect, dialyse, freeze, dry respectively the sod protein through deae - sepharose column chromatog - raphy ; process the sod protein through sephacryl s - 200 column chromatography with the preceding method. at last, process the pure sod protein with same functional enzyme electrophoresis and active dye, isoelectric focusing electrophoresis and sds - page

將粗酶液過deae -瓊脂糖柱層析，得三個活力峰，分別收集、透析、乾燥濃縮后；再上sephacryls - 200凝膠柱層析，按與deae -瓊脂糖柱層析后同樣的方法收集處理。

Basic principle of sds - 20 blood dialysis system

20型血液透析系統的基本原理

Analysis of copper binding protein by sds - page, three main protein bands observed. the main bands were digested by lysyl endopeptidase and isolate different peptides by hplc. 4

Sds page分析得到3條主要蛋白帶，剪下這三條帶進行膠內賴氨酸內切酶的消化，通過高效液相色譜分離肽段，選擇性進行肽段的氨基酸序列測定。

The sds - page results showed the fusion protein was efficiently expressed in the soluble form. 3 ) the expressed fusion protein was purified and cleavaged by enterokinase to release the mutation i of cmiv and the mutation ii, whiches exhibited antibacterial activity to the ecoli. k12

三、對以上表達的融合蛋白進行初步純化以及利用enterokinase切割融合蛋白，並進行抗菌活性檢測，結果表明所設計的cmiv突變體具有抗菌活性。

To prove up ulteriorly the components of ants, the author has separated and purified the polypeptides of formica rufa linnaeus and studied their biologic and officinal activities. at the same time, the author has mensurated the molecular weight of polypeptides by sds polyacrylamide electrophoresis to analyze the polypeptides quantific - ationally. in order to provide the scientific basis for studying and empoldering the ants, we have done these studies

為進一步探明螞蟻體內的活性成分以開發和利用其藥用價值，本文對紅褐林蟻的提取物中的多肽進行了分離、純化並對其生物活性和藥用活性進行了研究，通過電泳測定了多肽的分子量，從而為進一步研究開發螞蟻提供科學的依據。

In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。

Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。

Examination of chromsome number of the hybrids ri showed the sum of both parent chromsome besides aneuploid. the hydrid cell line ri was characterized by isoenzyme analyses, sds - page, rapd and opine synthetase assay

雜種r _ 1細胞的染色體數檢查、冠癭堿檢測、同工酶分析，可溶性蛋白sds - page電泳和rapd分析結果，都證明了其雜種特性。

Trypsin, dispase or hypertonic saline is used in decelliztion. triton x - 100 and sodiumdodecylsulphate ( sds ) are effective detergent in removing cell debris, but it is unknom that which of these is more effective

在去除細胞碎片方面，文獻中介紹以用曲拉通（ tritonx 100 ）和十二烷基磺酸鈉（ sodiumdodecylsulphate ， sds ）為多，對于這兩種洗滌劑的選擇也有不同看法。

We treat the porcine skin by 0. 25 percent trypsin, 0. 125 % trypsin, 2. 5 u / ml dispase, hypertonic saline or hypertonic saline - trypsin / dispase. we find that after the skin has been incubated in 0. 125 percent trypsin for 24h at 4 ?, the cells in the skin are all disintegrated. there are no significant differentiation between the acellular matrix treated by 0. 125, 0. 25 perlent trypsin, 2. 5 u / ml dispase and hypertonic saline - trypsin / dispase. but the cell ca n ' t be removed by using the hypertonic saline - sds

本研究通過對0 25胰酶不同脫細胞時間處理、不同濃度胰酶處理、 dispase脫細胞法、 im 、 zm高滲鹽水脫細胞法、高滲鹽水和胰酶或dispase混合脫細胞法的比較確認採用0 12盼胰酶， 4 ， 244 。

Except the igy got from by the method 6 % octanoic acid combined with ammonium sulfate, there were over two tape in others sds - page electrophoretogram

獲得igy產量以海藻酸鈉?硫酸銨法最高，可達5 . 69mg ml ，其次是用3辛酸?硫酸銨和聚乙二醇法。

The recombinant protein with the molecular weight of 76 kd was mainly expressed as inclusion bodies in e. coli, which was identified by sds - page

Sds - page證明，重組融合蛋白的分子量約76kd ，並主要以包涵體的形式存在。

The analysis of sds - page indicated a fusion protein band at the site of 20 - 30kda appeared in the form of inclusion body

Sds - page分析表明，重組菌在分子量20 - 30kda處出現一高表達蛋白條帶，此誘導表達的蛋白在沉澱中以包涵體形式存在。

After quantification, the protein samples were separated by isoelectric focusing electrophoresis and then by sds - page

定量后，分別進行一維等電聚焦分離和二維聚丙烯酰胺凝膠電泳分離。

二 、 effects of calcium concentration on the membrane - bound gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation. the membrane - bound gq a was extracted from the retina of the macrobrachium rosenbergi with protein extract and was electophoresised by sds - page. the percent of the membrane - bound gq a was analyzed by tanon gis gel image disposal system

二、鈣離子濃度對光暗適應羅氏沼蝦感光細胞膜結合gq蛋白亞基的影響用蛋白質提取液提取膜結合gq蛋白亞基並sds - page電泳，利用tanongis凝膠圖像處理系統分析其含量。

In our experiment, after light and dark adaptation, the retina of the macrobrachium rosenbergi was respective incubated in high calcium solution, physiological solution and low calcium solution. we studied the effect of calcium concentration on the content and subcellular localization of gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation by sds - page technology and imunoelectron microscopy technology. our study results indicated : 一 、 effects of calcium concentration on the soluble gq protein a subunit in the photoreceptor cell of macrobrachium rosenbergi on light adaptation and dark adaptation

而鈣離子對gq蛋白亞基活性有無影響還未見報道。我們以光適應和暗適應條件下的羅氏沼蝦復眼視網膜為材料，分別用高鈣溶液、生理溶液、低鈣溶液孵育后，通過sds ? page電泳技術及免疫膠體金電鏡技術，研究鈣離子濃度對光暗適應時羅氏沼蝦感光細胞gq蛋白亞基含量的影響及亞基亞細胞定位的影響。