secondary fluorescence 中文意思是什麼

secondary fluorescence 解釋
次生熒光
  • secondary : adj 1 第二（位）的，第二次的；中級的 （opp primary）。2 副（的）；從屬的；附屬的；輔助的；補充的...
  • fluorescence : n. 熒光（性）。
  1. According to the gene sequence and secondary structure of hcv ns5b, we design the sirnas targeting ns5b gene following with the requirement for sirnas design from tuschl et. al and synthesize it from dharmacon company ; hepg2 cell stably expressing ns5b - egfp protein was trasfected by synthesized sirnas with electroportion, the non - transfected cell and non - specific sirnas transfected cell are c onsidered as control group ; inhibitory effect of sirnas was investigated by fluorescence microscope with dapi dyeing and by semi - quantitative rt - pcr

    然後根據dsrna設計原則，結合nssb基因的序列特徵，藉助生物信息學軟體設計了針對nssb基因的sirnas ，並交由公司化學合成；電穿孔法轉染上述穩定轉染的細胞克隆，同時分別以非特異的sirnas轉染組和空白轉染組為對照， dapi染色后通過熒光顯微鏡和內標化rtpcr檢測，初步證實了化學合成的sirnas可以特異阻斷nssb基因的表達。
  2. Based on the theoretical analysis and experimental researches, it is presented that the wider spectra are resulted from the many fluorophores with large numbers of vibrational energy levels on the ground level in the blood cells, and the reduction of the spectral intensity is due to the reabsorption of the blood cells and the energy transfer of the collisions between the fluorophore and another one or other macromolecule. on the other hand, when the concentration of the blood cells is increased, the reabsorption of the blood cells, the secondary fluorescence due to the reabsorption and the influence of the concentration on the energy levels of fluorophores are all the factors of the red - shifted spectral peaks

    在進行理論分析和研究的基礎上，提出了因血細胞中存在多種熒光團，且這些熒光團的電子能級上又存在大量的不同的振動能級，從而導致被激發的熒光團發出較寬的熒光光譜；血細胞濃度的增大，熒光團以及其他大分子之間的距離變小，造成它們之間因碰撞的能量轉移概率加大，因而易產生熒光猝滅，結果導致熒光強度的變小；血細胞溶液中重吸收所導致的熒光猝滅和二次熒光發射，以及血細胞濃度的變化對其中熒光團能級系統的影響都是導致熒光峰值波長「紅移」的原因；進而研究了led光誘導血細胞產生熒光光譜的機理。
  3. Planar laser induced fluorescence ( plif ) was used to study the spray / wall impinging in constant volume vessel. the experimental result show that when fuel spray impinging on the plane wall, wall jet was formed while fuel impinging on a plane wall with a bump, when the wall jet meet the bump, a secondary jet can be formed. some parameters affecting the secondary spray were studied, including bump height, the secondary impinging distance, impinging distance and injector parameters

    對傳統燃燒室和bump燃燒室內燃油空氣混合過程進行了對比研究，發現燃油撞壁以後在傳統燃燒室壁面形成壁面射流，這一層燃油濃度很高，很難與空氣混合，而在bump燃燒室內，壁面射流遇到bump后，會在空間形成二次射流，二次射流進一步能快速與空氣充分混合。
  4. By the use of cirular dichroism and fluorescence techonologies to predict the secondary structure of protein, to determine the secondary structure of a protein, and to explore the unfold - refold mechanism of a protein

    運用圓二色譜和熒光技術分析預測蛋白質二級結構，研究蛋白質的穩定性與蛋白質的變性復性動力學機制。
分享友人
分享到 Line

分享到臉書