selected clone 中文意思是什麼

selected clone 解釋
入選無性系
  • selected : adj. 挑選出來的；精選的。
  • clone : n. 1. 【生物學】純種細胞，無性系。2. 復製品；（幾乎）一模一樣的人。3. （不動腦筋）機械行事的人；機器人。vt. 把…培養為純種細胞；無性繁殖；〈比喻〉復制。
  1. At the outset they must contain several ramets of every selected clone.

    在開始時每個入選無性系必須包括幾個無性繁殖後代。
  2. At first, 1. 67 u g per well mcab all was coated on three wells of a plate, and then 1. 5 x 1011 phage virion was diluted and added, after incubating with the target, wash away unbound phage by tbst ( 0. 1 % tween - 20 ), the bound phage was eluted with ph 2. 2 tris - gly buffer and amplified, the specially bound phage was enriched by taking through addition binding / amplification cycles. ln the following cycles, the stringency of panning can be increased by raising the concentration of tbst or decreasing that of mcab all, collecting and titering the washing phage of last time and output phage in each round, the selective ratio and the false positive rate of each round were worked out, the gradually increasing of selective ratio and decreasing of positive rate shows that the panning was effective. after 4 rounds of panning, 11 phage clones were selected after competitive - ellsa, the dna samples of 8 positive clones and 1 negative clone were sequenced and all the foreign peptides inserted was also deduced, a clear consensus binding sequence emerged

    在本實驗中，利用隨機12肽庫對抗豬瘟病毒（ classicalswinefeverviruscsfv ）糖蛋白me2的單抗a11進行表位篩選，經過四輪篩選以後，隨機挑取11個克隆作競爭- elisa檢測，結果表明，所挑11個克隆中，有9個克隆能對me2蛋白和a11反應產生抑制作用，抑制率最高可達64 ； dna測序以後經過dnastar軟體分析，發現它們的核心序列為anwralsl ，該核心序列與豬瘟病毒e2蛋白的28 - 35位氨基酸ttwkeysh具有同源性；夾心- elisa檢測和western - blotting試驗均證明所挑陽性克隆能被a11所識別；人工合成含核心序列的多肽經間接elisa試驗證實，也能被a11識別。
  3. Calculated from the transformation efficiency. human recombinant intercellular adhesion molecule 1 was immobilized on the elisa plates. after 4 rounds affinity panning and elisa analysis, 5 positive phage clones that can bind to icam - 1 specifically were selected. these positive clones were sequenced and the amino acid sequence of 15mer were deduced. the ka of one positive clone was 7. 8710

    首次利用定量圖像分析的方法研究野生大熊貓的取食行為，結果顯示：其取食行為存在固定的模式，這一模式是在其身體發育的過程中逐步形成的。
  4. The total rna was purified from the germ in the liquid by the guanidine isothiocyantehod method, then the total rna digested by dnase that had not rnase was used for rt - pcr. i change the magnesium ion dencity in the pcr system in order to optimize the pcr condition. at the end i selected the magnesium ion density as 1. 25 mm. the production of rt - pcr was inserted directionally into pgem ? z ( ampr ). the pgem ? z ( ampr ) was used to transform e coli jm109. i got a positive clone through culling and identificatin. the dna sequence inserted into pgem ? z ( ampr ) was sequenced and blasted with the cdna sequence of the # - mannanase mature peptide that got from genbank

    分取誘導培養液中的菌體，用異硫氰酸胍法提取總rna ，總rna再經無rna酶的dna酶處理後用于rt ? pcr 。在pcr擴增目的基因時，通過優選擴增體系，使鎂離子濃度為1 . 25mm時rt ? pcr可順利地獲得目的基因，並能定向克隆到載體pgem ? 3z （ amp ~ r ）中。用克隆載體轉化宿主大腸桿菌jm109 ，通過篩選獲取陽性克隆子，對陽性克隆子進行酶切與pcr鑒定，並對載體中插入的目的基因進行測序。
  5. The characteristics of quantum computing and the mechanism of immune evolution are analyzed and discussed. inspired by the mechanism in which immune cell can gradually accomplish affinity maturation during the self - evolution process, a immune evolutionary algorithm based on quantum computing ( mqea ) is proposed. the algorithm can find out optimal solution by the mechanism in which antibody can be clone selected, memory cells can be produced, similar antibodies can be suppressed and immune cell can be expressed as quantum bit ( q - bit ). it not only can maintain quite nicely the population diversity than the classical evolutionary algorithm, but also can help to accelerate the convergence speed and converge to the global optimal solution rapidly. the convergence of the mqea is proved and its superiority is shown by some simulation experiments in this paper

    分析和探討了量子計算的特點及免疫進化機制，並結合免疫系統的動力學模型和免疫細胞在自我進化中的親和度成熟機理，提出了一種基於量子計算的免疫進化演算法.該演算法使用量子比特表達染色體，通過免疫克隆、記憶細胞產生和抗體相似性抑制等進化機制可最終找出最優解，它比傳統的量子進化演算法具有更好的種群多樣性、更快的收斂速度和全局尋優能力.在此不僅從理論上證明了該演算法的收斂，而且通過模擬實驗表明了該演算法的優越性
  6. Abstract : the characteristics of quantum computing and the mechanism of immune evolution are analyzed and discussed. inspired by the mechanism in which immune cell can gradually accomplish affinity maturation during the self - evolution process, a immune evolutionary algorithm based on quantum computing ( mqea ) is proposed. the algorithm can find out optimal solution by the mechanism in which antibody can be clone selected, memory cells can be produced, similar antibodies can be suppressed and immune cell can be expressed as quantum bit ( q - bit ). it not only can maintain quite nicely the population diversity than the classical evolutionary algorithm, but also can help to accelerate the convergence speed and converge to the global optimal solution rapidly. the convergence of the mqea is proved and its superiority is shown by some simulation experiments in this paper

    文摘:分析和探討了量子計算的特點及免疫進化機制，並結合免疫系統的動力學模型和免疫細胞在自我進化中的親和度成熟機理，提出了一種基於量子計算的免疫進化演算法.該演算法使用量子比特表達染色體，通過免疫克隆、記憶細胞產生和抗體相似性抑制等進化機制可最終找出最優解，它比傳統的量子進化演算法具有更好的種群多樣性、更快的收斂速度和全局尋優能力.在此不僅從理論上證明了該演算法的收斂，而且通過模擬實驗表明了該演算法的優越性
  7. One n - peptide - binding scfv clone was selected from the phage antibody library using affinity panning. the target antigen, n - peptide was biotinylated using photobiotin first

    N -肽是一種新發現的神經肽，其n端與阿片肽高度同源，可與阿片肽受體相互作用。
  8. Selected one of the 14 strains - s93, s93 dna was digested partially with sau3a i and 2 ~ 3kb fragments were collected and inserted into puc 18, then transformed into dh5 a. filtering the clone with hybridization in situ, a 1 kb frament clone has been cloned

    使用sau3ai對基因組dna進行不完全酶切，回收2 3kb片段，與puc18質粒連接轉化大腸桿菌，利用地高辛標記探針，使用菌落原位雜交篩選轉化子；篩選到包含有約1kb外源片段的轉化子。
  9. Correct clones were selected and plasmid dna was isolated and digested with saci and puvii. a dna fragment of about 2. 1kb was purified and labeled by dig - 11dutp as probe. at least 40 positive clones were obtained from human genomic library by in situ colony hybridyzation with this probe. among them one clone contains human serum album dna by sequence

    以pcr擴增的人血清白蛋白（ hsa ）基因片段為探針，從人的基因組文庫中雜交篩選的陽性克隆中，經測序分析，有一個克隆含有全長hsadna ，用從其它的陽性克隆中選取兩種dna片段，即dna修復基因hfen1和一段非編碼大片段cit987sk - 384d8 ，與人hsadna一起，進行顯微共注射，成功制備了轉多基因小鼠。
  10. 3. the cloning of a - amylase gene : the methods of shotgun, pcr and rt - pcr were selected to clone a - amylase genes from bacillus subtilis hn503, xanthomonas campestris pv. campestris 8004 and aspergillus oryzae, hn504 the recombinant plasmid with cloned gene was designated as phn504, phn503 and phn8004

    本研究分別選用了鳥槍克隆法、 pcr和rt - pcr克隆法，成功地克隆到枯草芽胞桿菌（ bacillussubtilis ） hn503 、野油菜黃單胞菌（ xanthomonascampestrispvcampestris ） 8004和米麴黴（ aspergillus口盡留『 ） hn504的價澱粉酶基因。
  11. After electrophorised on 1 % agarose gel, the pcr production was purified with agarose gel dna extraction kit. the segment was ligated with vector pmd18 - t and then was tranformed into the competent cell of dh5 a. a construction mstnd - pmd18t was generated by inserting the sequence of 254bp into pmd18 - t vector and selecting the sense clones. positive clone was identified by three ways : endonuclease digestion, pcr and sequencing. the result showed that the cloned sequence coincides with the designed sequence. this construction was digested with nco i and xho i and ligated the pet28a ( + ) vector digested with the same enzymes using dna ligation kit. the production of ligation reaction was transformed into the competent cell of bl21 ( de3 ). after 12 - 16 hours of culture, several colnes appeared on the plate. some positive clones were selected to extract their plasmid. these plasmids were digested by nco i and xho i and indentified by pcr. a contraction, mstnd - pet28a was generated. the result showed that the cloned sequence coincides with the designed sequence

    F _ 1長38bp ， r _ 1長36bp ，其它片段均40bp長， f _ 1和r _ 1片段兩端分別加上限制性內切酶nco和xho的識別位點序列。用成對單鏈片段進行延伸反應，然後用其他單鏈片段作為引物，進行pcr擴增，用dna快速純化回收試劑盒回收所得254bppcr產物，與pmd18 - t載體連接、轉化dh _ 5 。受體菌感受態細胞，利用藍白斑遺傳學篩選法篩選陽性克隆，提取其質粒，採用nco和xho雙酶切鑒定，獲得了254bp的片段；用pmd18 - t載體上的特異引物rv - m和m13 - 47進行pcr鑒定，獲得300bp的片段。
  12. In this thesis, the hplap gene was cloned into the ppiczaa plasmid and induced expressed in the protease - deficient strain p. pastoris smd1 168. the clone that could secrete hplap with enzyme activity was selected

    本研究將hplap基岡兌隆到spiczoa質粒中並在畢赤酵母屍pastorjs蛋白酶缺陷菌株smdi168中誘導表達，獲得了有活性的分泌型hplap 。
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