sequencing primer 中文意思是什麼
sequencing primer
解釋
dna測序引物- sequencing : 測序，序列測定
- primer : n 1 初級讀本，初學書；初階，入門（書）；（尤指宗教改革前的）小禱告書。2 【印刷】10點或18點活字的...
-
By means of discontinuous tris - hcl buffer system and poly aery 1 amide gel electrophoresis ( page ), zymogram of 23 species of 11 genera in 4 subfamilies mirinae, orthotylinae, phylinae and deraeocorinae are gained. by specific primer pcr and sequencing, 57 cyt b gene 433bp sequences are obtained from 34 species respectively belonging to 17 genera in 5 subfamilies mirinae, orthotylinae, phylinae, deraeocorinae, bryocorinae from 64 species of 23 genera which involved in this research, and 2 outgroup species of family anthocoridae as well. 1
首次通過特異引物擴增和基因測序的研究方法從被試的5亞科23個屬的64種盲蝽中獲得了盲蝽亞科、合墊盲蝽亞科、葉盲蝽亞科、赤爪盲蝽亞科和蕨盲蝽亞科bryocorinae5個亞科17個屬的34種盲蝽以及外群花蝽科anthocoridae2種花蝽的cytb基因序列57條。 -
One version of the dideoxy sequencing method can be used to sequence the 5'ends of rna molecules by "primer extension".
一種形式的雙脫氧序列測定法是通過引子的延長來測定RNA分子5』末端的序列。 -
To make the pcr primer that have adequacy enzyme sit flowed the sequence, ligate with the pgem - t easy after pcr. make sure the sequence by sequencing
經組織培養后，以pcr和rt - pcr法檢測再生煙草，共得到轉synnhap基因煙草4株， pbi121空載體浸染的對照煙草再生苗5株。 -
Three special bands as pcr products, obtained by using primer no. l, are reclaimed, and then used to cloning and sequencing. through homologous comparison with data from international nucleotide databank, the band ' s functions have been simply analyzed
對1號簡並引物擴增出的3條特異性條帶進行了回收、克隆和測序；與國際核苷酸數據庫進行了同源性比較，對特異性片段的功能進行初步的分析和確定。 -
On the bases of designing a primer pair, we obtained the coding domain sequence of rbp by pcr from cdna library. then the gene was cloned into pgem - t vector, the dna sequencing showed that the cloned gene was in agreement with the reported sequence. then the tageted gene of rbp was further subcloned into a procaryotic expression vector pbv220 and constructed recombinant plasmids was named pbv220 - rbp. in order to expresse rbp in procaryotic cell efficiently, the recombinant plasmids was introduced into e. colidhs a straints. expression of rbp was induced by temperature induction
本實驗在合成該蛋白基因上下游引物的基礎上，利用pcr技術，從人睪丸cdna庫中釣取目的基因，並克隆于pgem - t載體中，經序列測定證明與文獻報道基本一致，再將目的基因從重組克隆質粒中亞克隆于pbv220原核表達載體中，轉化宿主菌，經溫度誘導后，進行sds - page電泳分析，發現在約21kd位置上出現了一條明顯的蛋白帶，與預期相符。 -
Used long pcr primers as sequencing primer, the two sides of sequence of the long pcr product were separately sequenced one reaction by the direct sequencing method of pcr products, and the sequencing sequence were carried on constrasted analysis with sequence of 168 strain in genebank
一2片段克隆質粒為模板，採用skippcr方法，得到了約4 . 3kb產物，以ta克隆方式，插入pgem一t載體中。將經鑒定的陽性克隆質粒進行測序，結果表明: biob與biol基因間的5lbp的終止子序列已被刪除。 -
One version of the dideoxy sequencing method can be used to sequence the 5 ' ends of rna molecules by " primer extension "
一種形式的雙脫氧序列測定法是通過引子的延長來測定rna分子5 』末端的序列。
分享友人