serum medium 中文意思是什麼
serum medium
解釋
血清培養基-
Cercariae were collected, cultured in vitro and transformed to schistosomula in the rpmi 1640 medium with rabbit serum. the schistosomula were cultured in conditional media up to 96 hours. the number of schistosomula was counted and the death rate was calculated
將條件培養基與童蟲共培養， 96h內連續觀察計數，計算童蟲死亡率，與陰性對照比較，童蟲死亡率最高的基因池進入下一輪篩選。 -
Methods : cell culture in serum - free medium, indirect immunofluorescence cytochemistry were used
方法：採用無血清細胞培養技術，間接免疫細胞化學染色法。 -
Method : 120 rabbit vessels were randomly divided into three groups : the vessel of control group in cryopreservative medium containing calf serum were subjeted to rate - controled slow freezing ; those of experiment group i in cryopreservative medium containing mixed animo acids were frozen by the same method as in control group ; and those of experiment group ii in the same cryopreserative medium as in control group were subjected to rapid freezing
材料和方法：取中國純種家兔股動脈120段，隨機分為3組：對照組用含胎牛血清的保護液處理，以慢凍方式降溫；實驗組1用含復方氨基酸的保護液處理，以慢凍方式降溫；實驗組ii用含胎牛血清的保護液處理，用速凍方式降溫。 -
In this experiment, radio - immunoassay and hybridization in situ were applied to observe the insulinotropic activities of glp - 1 ( 7 - 36 ) nh2 and reveal the mechanisms underlying this process. methods : rat pancreases were removed from 3 - 5 day - old sprague - dawley rats and dissected into 0. 5mm3 segments and islets were isolated by the collagenase digestion method of wangling et al. thoroughly washed islets and suspended in modified rpmi - 1640 medium supplemented with 10 % fetal bovine serum, and added to 50ml cell culture flasks
方法：胰島的分離參照王玲等的方法，每次實驗取新生3 - 5天sd大鼠，無菌條件下剖腹取出胰腺，剪切為0 . 5mm ~ 3的組織塊， v型膠原酶消化30min后，離心洗滌，懸浮於完全培養基，接種入50ml培養瓶，於5 co _ 2 、 95空氣條件下培養20h ，轉板純化，接種於96孔培養板培養24h ，按實驗要求進行實驗。 -
Abstract : adopting the serum - free and animal - source - free medium domestication express cell efficiently, setting up to express system efficiently, suspending culture cell, can raise the cell density in the scale turn the production, strengthen the cell vitality, control cell to propagate level, extension cell culture period, increase the target protein of yield, raise product quality, simplification of produces technics, reduce production cost, then raising the efficiency that the scale turns culture
提要：採用無血清無動物組分培養基馴化高效表達細胞，構建高效表達系統，懸浮培養細胞，可以在規模化生產中，提高細胞密度，增強細胞活力，控制細胞增殖水平，延長細胞培養周期，增加目標蛋白的產量，提高產品質量，簡化生產工藝，降低生產成本，進而提高規模化培養的效能。 -
Various concentrations of rrta and rrta - yqrl were added into the wells and incubated for 6 h to allow internalization of toxins by fluid - phase endocytosis. the cells were then incubated in rpmi - 1640 medium containing 10 % fetal calf serum for another 24 h
結論本實驗用基因工程方法原核表達了rl 』 a蛋白和rl 』 a一yqrl蛋白，利用rta蛋白和f3ga的結合特性，溫和、簡單、有效地洗脫了目的蛋白。 -
Methods rabbit conjunctival epithelial tissues were explanted in serum - free system the morphology, gowth, proliferation and differentiation of outgrow cells were compared with those in serum - containing medium
方法分別用含胎牛血清培養液和不含血清培養液進行組織塊培養，觀察兩種培養方法在細胞形態、細胞生長情況和增殖及細胞分化方面的差異。 -
The cell - matrix complex was incubated with dmem and 10 % bovine serum under condition of 37, 5 % co2 - the medium was changed daily. ( 3 ) cell - matrix complex paraffin section was made after 7 days incubation
將細胞懸液接種于戊二醛交聯的膠原一殼聚糖多孔膜，加dmem 10小牛血清培養基後放置37 』 c ， 5 co 。 -
Thus, the success of nscs therapy is determined by the induced differentiation and the effect of micro - enviroment on the survival, proliferation and differentiation of it. the isolation and culture of nscs in vitro successfully is the presupposition for its research. to avoid the influence of unknown factors exiting in serum on nscs " differentiation, serum - free medium with egf and bfgf as mitogens to stimulate proliferation and inhibit differentiation in vitro was introduced through a long period of search
澱粉樣蛋白（ amyloidpeptidy ， a ）是澱粉樣蛋白前體蛋白（ amyloidprecusorprotein ， app ）異常代謝產生的含有39 ? 43個氨基酸殘基的疏水肽，可聚合成不溶的纖維形式或不定型顆粒狀結構，導致各種毒性反應，在阿爾茨海默病（ alzheimer ' sdisease ， ad ）發病中起著至關重要的作用。 -
Results the epithelial cells cultured in serum - free medium not only demonstrated a significant increase in proliferative, colony - forming efficiency and cell generations, but also have the normal structural and functional properties than those in serum - containing medium
結果無血清培養系統中結膜上皮細胞的增殖、克隆形成及細胞傳代能力均較含血清培養系統高，而且具備結膜上皮細胞全部組織結構及功能特點。 -
The eg cell culture media consisting of dmem medium supplemented with fbs, chicken serum, beta - mercaptoethanol, l - glutamine. hepes, chicken embryonic extract and cytokines etc. after 24 hours culture, the isolated pgcs were selectively attached on the gonadal stromall cells in the plates
加入新鮮的eg細胞培養液（含dmem 、胎牛血清、雞血清、 -巰基乙醇、 l -谷氨酰胺、 hepes 、雞胚浸出液以及細胞因子等成分）培養24小時以後， pgcs開始部分貼附於共培養的生殖原基細胞上。 -
The results showed that : when cultured in the medium of m199, supplemented with 20 % bovine serum containing a moderate amount of antibiotics, the incubtion ph 7. 2 - 7. 4, the culture temperature 28. the primary culture cells formed the dense single wall within three weeks, the generation time of the subculture cells was 5 - 6 days, most cultured cells were fibroblastic - like cells with few epithelial - like cells
研究初步表明：在m199培養基加入20小牛血清（常規量雙抗） ， ph在7 . 2 - 7 . 4之間， 28的培養條件，四倍體鯽鯉魚腎組織原代培養三周左右即可形成緻密單層，傳代細胞為5 - 6天左右傳一次。培養細胞以成纖維樣細胞為主，有少數上皮樣細胞。 -
We report a novel three - stage method to efficiently direct the differentiation of human embryonic stem cells into hepatic cells in serum - free medium
我們報道一種新的三步法有效誘導人胚胎幹細胞在無血漿培養液中向肝細胞分化。 -
2. cytotoxicity assay hela, skov - 3, and wish cells were plated out in 96 - well plates at a density of 1. 0x104 cells per well in the serum - free rpmi - 1640 medium
值后發現和rl 』 a蛋白相比較， rta一yqrl細胞毒性作用明顯提高，分別是前者的2 ， 10和40倍。 -
Study on serum - free medium for 5b1 cell growth
1細胞生長的無血清培養基的研究 -
Medium which is serum - free or in the low concentration serum inhibit the proliferation of cells. a certain degree of increase serum concentration can promote the growth rate as well as prolong the life. the feeder layer cells were provided by mef, mue ( mouse uterus epithelium ) and rmc ( rat myocardial cells )
Mef和es細胞體外培養血清濃度以15 20為宜，試驗中以mef 、 mue 、 rmc作飼養層，結果表明：以12 . 5dpc的mef為飼養層，無論是在小鼠es細胞的原代培養，還是在克隆傳代過程中，效果都是最佳的。 -
To initiate induction, undifferentiated escs were dissociated and replanted on none - coated tissue culture plastic dishes in nsc medium. the medium was changed ever - y other day into serum - free nsc medium
誘導分化時去飼養層細胞，重新接種于無包被的培養皿中，使用nsc培養液，逐漸更換為無血清nsc培養液。 -
A successful cell strain for biopharmaceutical production must conform to the following characteristics : the cells could produce high level of recombinant proteins ; the cell should be adapted to serum - free or protein - free medium ; the cells should be resistant to adverse culture conditions, which means the cells should have some anti - apoptosis property ; if not cultured i n suspension, the cells should also be able to grow in adherence
一株成功的工程細胞除了要求目的蛋白的表達量高外，還必須適應無血清培養基培養；必須具有對不利環境的抵抗能力，即抗凋亡能力；對于非直接懸浮培養的細胞，還必須具備在無血清培養條件下的貼壁能力。 -
The stable expression cell line was selected. expression level of the line cultured in serum - free medium was 580iu 106 cell 24h. the product expressed was purified by zn
篩選到的穩定表達株在無血清培養基的表達量為580iu 106細胞24h 。 -
Culture and amplification of neural stem cells of mice with serum medium containing basic fibroblast growth factor
血清培養基輔以堿性成纖維細胞生長因子培養擴增小鼠神經幹細胞
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