shotgun method 中文意思是什麼
shotgun method
解釋
散彈槍法-
The shotgun method was only used with b. subtilis hn503. the optimun conditions for total dna extraction, enzyme digest, ligation and transformation were studied the results showed that the most important factor was transformation efficiency. since the concentration of ligation products was low in common
在已知基因序列的條件下用pcr和rt一cr克隆基因時,對pcr和rt一pcr的反應條件、產物的回收、回收產物的酶切、酶連和轉化等條件進行了一系列的比較研究。
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