southern blot 中文意思是什麼
southern blot
解釋
southerndna印跡-
Transformed shoots were selected on solidified medium containing kanamycin. ten kanamycin resistant transformants were obtained by direct or induction calla. these transformants were checked by pcr, pcr - southern blot and southern blot, confirmed that two positive transformants were integrated into the genome of boechmeria nivea l guad
對所得到的抗性轉化株進行了pcr 、 pcr - southernblot 、 southernblot檢測，其中2株呈陽性，證明vp4基因已整合到苎麻基因組dna中。 -
We produced transgenic tomato plants by an agrobacterium tumefacien - mediated method. pcr - southern blot and rt - pcr revealed integration of afgf gene in the plant genome and successful its transcription in transgenic plant cells
我們利用農桿菌介導的轉化方法獲得了afgf轉基因番茄植物，並且通過pcr - southern 、 rt - pcr證明了afqf在宿主基因組中的整合及表達。 -
Transgenic plants were comfirmed by molecular determination including pcr, southern blot and rt - pcr. 4. functional analysis and iron content of transgenic plant crude proteins of transgenic plant were extracted from leaves, and then used to test its antibiotic activities
4 .轉基因植株抗菌能力加強，鐵含量得到提高提取轉基因植株葉片粗蛋白，以大腸桿菌dhsq和酵母菌ahiog為試驗靶標，側定了轉基因植株粗蛋白的抑菌活性。 -
The analysis results of southern blot hybridization among all of specimens are coincidence with the detecting results of pcr products electrophoresis
所有標本進行southernblot雜交分析，結果予以證實。 -
Fingerprints of 5 strains of the inbred mice and 2 strains of the inbred rats was conducted using a nonisotopically hrp labeled jl - 02 by the second institute of the public safety bureau of china and southern blot hybridization, the author studied many fingerprints of the same dna, the different organic fingerprints of the same organism and fingerprints of parent and offspring. the patterns were completely different among the different strains and those of the samples from the same strain were completely identical
採用公安部二所自行研製的jl - 02多位點探針對5個品系的近交系小鼠和2個品系近交系大鼠進行了dna指紋分析，經過對同一dna的反復製作dna指紋圖和同一個體不同組織進行的dna指紋圖製作及對親代和子代（同品系內和不同品系間雜交）間的dna指紋圖比較。 -
This gene, artificially put under the control of camv 35s, was introduced into tobacco with the aid of agrobacterium, and the transgenic plants obtained were identified by gus staining, pcr and southern blot analysis
將arge與組成型啟動子camv35s相連，構建植物表達載體，通過農桿菌介導轉化煙草。經過gus染色、 pcr及southern鑒定獲得了轉基因植株。 -
Hybridization bands were detected by southern blot analysis using lea3 gene probe labeled by digoxigenin the result showed that foreign lea3 gene integrated into strawberry genome
2 。對點雜交為陽性的植株進行southernblot分析，轉化植株檢測到了雜交帶，除預期的1 -
7. we found ent - kaurene oxdase belong to a multigene family by southern blot. we deduced that multigene response to different development signal
這預示著此酶在植物生長發育過程中起著多種作用，不同的基因可以響應不同的發育信號。 -
Southern blot analysis shows that there are two ecbp21 gene copies in genome
Southernblot檢測表明白芷基因組中可能存在兩個ecbp21基因拷貝。 -
Founder mice will be examined by polymerase chain reaction ( pcr ) and southern blot
採用聚合酶鏈式反應（ pcr ）和southern印跡對陽性鼠進行檢測。 -
Pcr, pcr - southern blot analysis, southern dot blot analysis of lettuce dna confirmed that adw gene had been integrated into the plant genome. the results also showed that the transformation frequency of pb - adw was higher than that of pbg - adw, which suggested that camv35s promoter would be better than pi ii promoter in the case of transgenic lettuce
（ 3 ）細胞核載體pb - adw 、 pbg - adw均採用農桿菌介導法將adw導入萵苣，細胞核轉化獲得了生長良好的抗性萵苣植株，經pcr 、 pcr - southern 、 southern斑點雜交分析證實， adw基因已整合到萵苣基因組中。 -
3. southern blot analysis of pcr positive plants indicated that 3 plants were transgenic plants and the thymosin gene has been transformed into genome of lettuce. 4
Ctab法提取pcr陽性株的基因組dna進行southern雜交，其中3株為陽性，表明胸腺肽基因已經整合到生菜基因組中。 -
Then, we transformed those two genes into tomato and tobacco plants via agrobacterium tumefaciens. after verified by antibiotic resistance, reporter gene examination, southern blot detection, and genetic segregation analysis, we obtained 3 and 7 transgenic tobacco plants with one copy of rbcs 3a - gus or rbcs 3c - gus gene, respectively. further, we established two suspension - cultured cell lines using above mentioned two kinds of transgenic tobacco plants
對得到的再生煙草植株分別進行了報告基因表達水平檢測、 southern - blot鑒定以及t _ 0代轉基因種子遺傳分離規律分析，分別得到了單拷貝的穩定表達番茄rbcs3a - gusa 、 rbcs3c - gusa和35s - gusa基因的轉基因煙草3株、 7株和1株，同時還得到單拷貝的只轉化gusa基因的陰性對照轉基因煙草3株。 -
According to the southern blot results, a 6. 5 kb bamh i restriction fragment containing the gene coding for hal c8 was cloned and sequenced
Halcs的開放閱讀框為849個核昔酸，編碼283個氨基酸;它必須要剪切掉n端的197個氨基酸才能成為成熟的蛋白。 -
A dna fragment of 348 - bp amplified from the b subunit gene was cut into two dna fragment of 216 and 132 - bp by haelli. endonuclease restriction analysis of the plasmid content with psti showed that strainas with the same result of southern - blot with spesific probe had the different cleavage pattern. the isolated 285 - bpand 348 - bp dna was ligated with plasmid puc18. the ligation mixture was used to transform e. coli jm109and the transformants were plated on lb agar containing antibiotics. plasmid dna containing cloned genes were used for direct sequencing
提示1999年的疫情由不同的病原菌引起。另外使用針對志賀毒素2及其變種的引物對進行pcr檢測、細菌染色體pst和pcr產物的hae 、 ras酶切分析，以及pcr產物的序列分析，發現2000年從江蘇省徐州市患者和家畜家禽糞便標本分離的大腸桿菌o157 ： h7菌株僅僅攜帶slt2vha基因。 -
Osdd2 gene existed as a single copy gene in the rice genome based on the result of southern blot analysis. we took a pcr method to clone the cdna of osdd2 gene
使用克隆的邊端插入序列為探針對水稻（中花11品種）進行southern雜交分析，表明osdd2基因在水稻基因組中是以單拷貝存在。 -
The genomic dna extracted from three rubber tree strains ( pr107, rrim600, gt1 ) was digested with hinckr. the southern blot was made with the same probe as above. the result showed that all the three strains turned up only one brand ( - - 4. 8kb ). the three strains had the same inherent background
用橡膠樹pr107 、 rrim600 、 gti三個品系的基因組洲a分別經hinan酶切后，進行了southern雜交。結果表明，三個無性系都只出現一條雜交帶，位置一』致（ 4 -
I and hindlll respectively. after the digested products were run via agarose gel electrophoresis and transferred into nylon membrane, the southern blot was carried out using the cdna of rubber tree etrl as probe. the result of the southern blot showed that a hybridization band ( - 3. 0kb ) turned up from the ecor i digested product and another band ( - 4. 8kb ) turned up from the hindi ]
從橡膠樹pr107品系的嫩葉提取基因組dna ，用限制性內切酶ecor 、 hinofll分別酶切，瓊脂糖凝膠電泳分離並轉膜后，用克隆的橡膠樹etri基因的cdna片段作探針進行southern雜交分析，結果表明， ecor酶切在約3 okb處有一條雜交帶出現， hi 。 -
Methods : extracting the total rna of human pbmc, the objective include 60 healthy blood donator, 30 patient with viral encephalitis and multiple sclerosis and parkinsonian syndrome, 30 patient with schizophrenia and affective disorder, this indviuals were inpatients or outpatients of the first hospital of chongqing university of medical science from december, 2000 to june, 2001. using nested rt - pcr techique to detect borna disease virus " middle fragment in orf i, and using southern blot hybridization to analyze the pcr product
重慶醫科大學碩士學位論文方法：提取從2000年12月至2001年6月在重慶醫科大學第一附屬醫院神經科和精神科住院及門診的60例健康獻血者、 30例包括原因未明的病毒性腦炎、多發性硬化、帕金森綜合征，以及30例包括精神分裂癥和情感障礙患者pbmc中的總kn 』 a ，採用套式逆轉錄聚合酶鏈反應estedrticr ）技術進行了bdvorf基因中部片段的檢測，並對pcr產物進行電泳分析及southernblot雜交分析。 -
The transgenic goldfish were detected and screened by pcr, southern blot. the results showed that pcr amplify a 478bp specific molecular band in 5 individuals, southern blot results showed a strong signal in transgenic fish
Pcr檢測結果顯示，有5尾轉化個體的總dna能擴增出一條478bp的特異性分子帶，說明轉化個體整合有gfp基因，整合率為42 。
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