subtilisin 中文意思是什麼
subtilisin
解釋
枯草溶菌素-
Based on the extensive studies of subtilisin - like protease ( prl ) of metarhizium anisopliae, extracellullar serine protease is suggested to be a key enzyme involved in the fimgal penetration to invertebrates. the investigation of serine protease in the nematode infected by owvtl may help to understand the mechanism of nematophagous fimgi as biological control agents. a 3l kda serine protease was isolated and purified from the liquid culture of h rhossiliensis owvtl challenged with nematode panagrellus redivivus
本研究利用線蟲誘導下owvt - 1菌株液體發酵，通過粗分級分離、離子交換層析和凝膠過濾層析分離提純了一個分子量為31kda的絲氨酸蛋白酶，生物學測定表明其對大豆胞囊線蟲二齡幼蟲具有致死作用，同時測定了該酶理化特性，酶活力在75附近酶活力最高，隨著ph的增加酶的穩定性升高，與膽堿酯酶具有相似的ph曲線，對特異性底物aape （ suc - ala - ala - pro - glu - pna ）具有作用， ssi和ci - 2抑制該酶的活性。 -
The sequence around these residues revealed that ap was a new member of the subtilisin family
該蛋白酶可以認為是枯草桿菌蛋白酶家族（ subtilisin ）的新成員。 -
A pair of primers, based on the high homologous n - and c - terminal amino acids sequences of subtilisin of bacillus pumilus, were designed and used to amplify subtilisin gene from genomic dna of 4 different bacillus strains by polymerase chain reaction
Sds 、 edta和pmsf對酶活力有不同程度的抑制作用。以4株芽孢桿菌的總dna為模版，利用擴增短小芽孢桿菌堿性蛋白酶基因的引物對它們進行pcr擴增。 -
Mutants of subtilisin e
的突變體 -
Enzymatic technology and analysis of enzymatic products of earthworm with subtilisin from bacillus subtilis as1
蚯蚓外源酶與內源酶酶解產物分析 -
Based on the high homologous n - terminal amino acid sequence between ba - dfe and subtilisin bpn, the fragment encoding ba - dfe mature peptide was amplified from the total dna of b. amyloliquefaciens dc - 4 by pcr
根據ba - dfe的n -端序列與subtilisinbpn高度同源設計引物， pcr擴增出ba - dfe成熟肽編碼區片段。 -
In the same method, another pair of primers weredesigned in order to amplify subtilisin carlsbsrg from genomic dna of those bacillus strains by pcr. the cloning and sequencing of a fragment from bacillus licheniformis dy. are under investigation
又用擴增地衣芽孢桿菌堿性蛋白酶基因的引物在b . licheniformisdy的總dna中擴增到一條與預期大小相一致的片段，進一步實驗仍在進行中。 -
Due to high homology of nucleotide between ba - dfe and subtilisin bpn, primers were designed and synthesized. the intact ba - dfe gene was amplified by pcr and cloned. the sequence analysis indicated that the ba - dfe gene has an open reading frame with 1146bp, which encodes 382 amino acid residues containing signal peptide, pro - peptide and mature peptide
序列分析顯示，該片段的核昔酸序列中含有1146hp的開放閱讀框，可編碼382個氨基酸殘基的ba dfe前體蛋白，包括30個氨基酸殘基組成的信號肽、 77個氨基酸組成的前導肽和275個氨基酸殘基組成的成熟肽。 -
The gene encoding the mature peptide was cloned from the total rna of h rhossiliensis owvt1 by rtpcr. sequence analysis of the gene was described in this papel the amino acid sequence, as derived from the nucleotide sequence of a cdna clone, had high homology with other subtilisin - like serine protease of nematogenous fimgi
與其他絲氨酸蛋白酶基因序列比較表明，與其他線蟲卵寄生性真菌如paecilomyceslilacinus 、 verticilliumchlamydosporium及m . anisopliaevar . anisopliae同源性較高，而與捕食線蟲真菌arthrobotrysoligospora同源性較低（ 45 ） 。
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