supernate 中文意思是什麼
supernate
解釋
浮在表面的液體層-
The supernate of virus culture was used as templet in overlapping pcr, then the interesting gene was obtained
利用重組pcr的方法，以病毒培養液上清為模板，把二個目的基因串聯在一起。 -
Multicopy integrants were screened with g418 from pichia pastoris which contains recombinant plasmid, and induced with methanol to secrete interesting peptide. the supernate of pichia pastoris culture was analysed by sds - page and western blotting. a reactive band, which the apparent molecular weight is 36kd, can be detected with sheep anti - hcmv polyclonal antibodies
重組質粒轉化巴氏畢赤酵母， g418篩選出多拷貝插入的單克隆，甲醇誘導多拷貝插入的單克隆酵母細胞分泌目的蛋白，培養液上清經sds - page電泳分析，在蛋白質印跡中檢測到培養液上清有一表觀分子量為36kd ，能與羊抗hcmv多克隆抗體發生發應的條帶。 -
The supemate of cell culture was collected 48 hours after transfection. this supernate was also called conditional media
在適當條件下收集尾蚴，體外培養使其脫尾變成童蟲。
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