template strand 中文意思是什麼
template strand
解釋
模板股-
In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis
擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。 -
As bases are added by polymerase to the starting point of a new complementary strand, known as a primer, or recognized by ligase as a match, the template ' s sequence is revealed
當聚合酶將一個核苷酸加在新互補鏈的起始引子之後，或接合酶認定某段核苷酸鏈與原始模版配對，就可利用這些反應來得出原始模版的序列。 -
The dna encoding the desired protein was generated as a bamh i - sal i fragment using pcr. the template was pbv220 - hgf - a strand that had been constructed in our lab
其中gst是一種純化標簽，可以在後續的蛋白分離純化中使用gst親和層析的辦法純化目的蛋白，這將使純化工作變得簡單而有效。 -
The ns5 protein of dengue virus is the largest molecule encoded by the virus genome with a molecule weight of 104 000. recent research work indicates that the ns5 protein acts as the rna - dependent rna polymerase ( rdrp ) in virus which has the ability to recognize and bind its template rna to synthesize a complementary strand
近年來的研究表明， ns5蛋白具有rna依賴的rna聚合酶（ rdrp ）功能，即可以識別具有相對特異性的模板rna並與之結合，合成與模板互補的rna鏈，在病毒基因組復制過程中起關鍵作用。 -
Some investigators, however, are working on ways to detect fluorescent signals emitted from just one template strand molecule
然而有些研究人員正想辦法讓靈敏度達到可偵測一個模版分子所發出的信號。 -
To investigate the silence effect of hela cells " telomerase gene expression after shrna based on human telomerase htert transfected into cells. methods : we constructed a partial double - strand dna with t7 promoter as dna template and synthesized small hairpinrna in - vitro using t7 rna polymerase
方法：根據端粒酶htert基因1573 ? 1591位的核酸序列，構建帶t7啟動子的部分雙鏈dna模板，用t7rna聚合酶體外合成短鏈shrna 。 -
Conclusions : the in - vitro method that partial double - strand dna with t7 wi l. - toi promoter sequence as template and synthesizing by t7 rna polymerase can product high yield, excellent purity shrna. lt is a convenient -, effective ^ low - cost method and fit for small rna synthesis and rna interference researches in ordinary laboratory
結論：以帶t7啟動子部分雙鏈dna為模板，用t7rna聚合酶體外合成出的shrna產量較高，純度較好，是一種簡便、高效、低成本的短鏈rna的制備方法，適合於普通實驗室用來進行短鏈rna的合成和rna干擾實驗。
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