terminator 中文意思是什麼

The terminator is the boundary between day and night.

地影界線就是白天和黑夜的邊界。

In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。

Gobang terminator is a gobang the game, nothing to play down the play

五子棋終結者，是個五子棋的小游戲，沒事就下來玩了玩。

Terminator 2 : judgement day grossed $ 500 million, while terminator 3 took $ 427 million around the world

《終結者2之審判日》的票房總收入達到5億美元，而《終結者3 》的全球票房也達到4 . 27億美元。

Considering alcaligenes faecalis pencillin g acylase ( afpga ), which possesses the attractive characteristics for beta - lactam antibiotics conversions, the gene of pga was cloned into two expressing vector pkkfpga and psmlfpga. both the constructed plasmids psmlfpga and pkkfpga contained the pga gene, trc promoter and rrnb transcript terminator, differ in the replicon and antibotic marker, pkkfpga contained multicopy replicon ( cole 1 ) and ampicillin marker. while psmlfpga contained medium - copy replicon ( p15a ) and tetracycline marker

本文將糞產堿桿菌青霉素g酰化酶（ afpga ）基因構建表達載體pkkfpga和psmlfpga ， pkkfpga和psmlfpga均含trc啟動子、青霉素g酰化酶基因、 rrnb轉錄終止子，其中pkkfpga含有氨卞青霉素抗性基因和cole1高拷貝復制子；而psmlfpga含有四環素抗性基因和p15a中拷貝復制子。

J mol biol., 1992, 224 : 53 - 63. 26 abe h, aiba h. differential contributions of two elements of rho - independent terminator to transcription termination and mrna stabilization. biochimie, 1996, 78 : 1035 - 1042

通過計算，我們預測到266個不依賴終止子，其中包括232個蛋白編碼基因， 12個trna基因和3個rrna基因，約17 %不依賴終止子位於操縱子的末端。

As a rule this concerns only part of the whole terminator.

通常這只是影響地界線的一部分。

Of particular importance to the observer is the position of the terminator.

對于觀測者特別重要的是明暗界線的位置。

Planetary terminator games - 4455 miniclip games

行星終結者小游戲- 4399小游戲

Xiaoqiang terminator games - 4455 miniclip games

小強終結者攻略- 4399小游戲網

Followed by a line terminator to the text stream

的文本表示形式寫入文本流。

Optional transact - sql statement terminator

可選的transact - sql語句終止符。

Seagull terminator games - 4455 miniclip games

海鷗終結者小游戲- 4399小游戲

Writes a line terminator string to the output stream

將行結束符字元串寫入到輸出流。

Wildcat terminator games - 4455 miniclip games

野貓終結者小游戲- 4399小游戲

Theftproof terminator, key

防盜接負載鑰匙

2. in order to express chs in plant, we constructed plant expression vector p1301b q c. first added camv 35s promoter and nos terminator to chs by one median clone

（ 2 ）為了能在在植物中表達（ chs ，通過一步的中間克隆，將chs連上camv35s啟動子和nos終止子。

The minimum size of the character buffer in characters. this value does not include space for a null terminator

這個我看了，還是不懂，既然說最小長度，那就有個比較的過程吧，這個比較的過程我不懂。

The amplification system was optimized so that the pcr with different primers can be carried out under the same condition. the pcr products were sequenced using sanger ' s terminator and fluorescent label techniques. sequences were analyzed and compared base on sequencing analysis3. 4 and seq / ede software

方法根據mtdna控制區及其周圍區域的序列，設計多對引物，探索優化擴增體系，使擴增條件能夠同時滿足多對引物的需要，用sanger末端終止法及熒光標記技術對樣本進行dna測序， sequencinganalysis3 . 4和seq ede軟體進行序列分析和比對。

This is one of a series of images designed to look for landforms near europa ' s terminator - the line dividing day and night - where low sun angles highlight subtle topographic features

這是一系列被設計用來觀察木衛二晨昏線附近地貌的圖片中的一幅，晨昏線是分隔白天和夜晚的界限，那裡陽光以低角度突出了精細的地貌。