trans-golgi 中文意思是什麼

trans-golgi 解釋
轉運高爾基氏體
  • trans : transimitter的簡寫
  • golgi : n. 1. 高爾基〈姓氏〉。2. Camillo Golgi 〈1844-1926，義大利解剖學家與病理學家〉。
  1. Translocation from endocytic compartments to the cytosol is the essential and rate - limiting step in the intoxication process of most toxins such as ricin, diphtheria toxin, shiga toxin and pseudomonas exotoxin ( pe ). a number of these toxins are transported to trans - golgi network ( tgn ), and in many cases such transport to the tgn is required for the translocation and cytotoxicity. in deed, 5 % of the ricin endocytosed by cells has been shown to reach the tgn

    蓖麻毒素進入細胞的機理不甚明了，一般認為是rtb先與細胞膜受體結合，主要經過受體介導的內吞作用進入吞噬體，然後沿著內體、高爾基體、內質網等逆向分泌途徑，有序地運輸到內質網，最後從內質網轉位進入細胞漿，在胞漿內攻擊核糖體，從而抑制蛋白質的合成，導致細胞死亡。
  2. Rab7 mediates transport of proteins from early endosome to late endosome or fusion of early endosome with late endosome, while rab9 mediates the retransport of m6pr from late endosome to trans - golgi network. the gtp / gdp - binding ability and intrinsic gtpase activity of rab proteins make them exist in rab - gtp or rab - gdp status. the switching of rabs between gtp - bound ( active ) and gdp - bound ( inactive ) forms requires other regulatory proteins, including guanosine exchange factor ( gef ), gdp dissociation inhibitor ( gdi ) and gtpase activating protein ( gap ), and lays the foundations of rab function

    Rab蛋白家族成員和其他小g蛋白一樣可以結合gtp gdp ，在自身gtp酶的水解作用和其他蛋白（ gef 、 gap 、 gdi ）的調節作用下發生gtp （活化狀態）和gdp （無活性）結合形式的轉變；同時rab蛋白也在膜結合性（ membrane - bound ）和胞漿可溶性（ cytosolic ）之間變化，一個變化過程對應著一個功能循環，接著rab蛋白回到初始狀態參與下一個循環。
  3. These results suggest that the cytoplasmic dynein intermediate chain - like protein exists in lily pollen, and it distributes mainly on either cis - and trans - golgi - associated vesciles

    以上的結果表明類細胞質力蛋白中間鏈存在於百合花粉及花粉管中，並且在百合花粉管中順面和反面高爾基體附近的囊泡膜上均有分佈。
  4. By sds - page and immuno - blotting, the monoclonal antibody of anti - chick brain cytoplasmic dynein intermediate chain could recognize the 67 kda protein in purified golgi apparatus fraction from lily pollen. subsequently by immuno - gold labeling and transmission electron microscopy, we found that the dynein intermediate chain - like protein bound mainly to the membranes of golgi - associated vesicles. statistics analysis of dynein intermediate chain - like protein on golgi - associated vesciles showed the nearly equal chance of distribution on either cis - or trans - golgi - associated vesciles

    對分離純化的百合花粉及花粉管中高爾基體組分進行sds -聚丙烯酰胺凝膠電泳和免疫印跡發現，抗雞腦細胞質力蛋白中間鏈單克隆抗體在67kda處有較強的免疫交叉反應；進而通過免疫金標結合電子顯微鏡觀察發現，大多數類細胞質力蛋白中間鏈存在於高爾基體附近的囊泡膜上；統計結果表明，類細胞質力蛋白中間鏈在順面和反面高爾基體附近囊泡膜上的分佈機率大致相等。
  5. It is known from studies using brefeldin a ( bfa ) that disruption of tgn can block the cytotoxicity of ricin, suggesting that the intracellular compartment may be an important part of the uptake pathway. ricin enters the cells by receptor - mediated endocytosis, followed by translocation across the membranes of intracellular organelles. in this study, a trans - golgi retention peptide signal yqrl was fused to the c - terminus of ricin a chain ( rta ) by polymerase chain reaction

    本實驗為重點研究反式高爾基體在毒素細胞內轉運過程中的作用，我們設計了一個反式高爾基體的靶向信號肽基因yqrl （ tyr - gln - arg - leu ）連接在rta分子的羧基端，用原核表達蛋白體系，純化並測定rta - yqrl與rta蛋白對hela （人宮頸癌細胞） ， wish （人羊膜上皮細胞） ， skov - 3 （人卵巢癌細胞）三種細胞的毒性作用。
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