transfer protein 中文意思是什麼
transfer protein
解釋
轉移蛋白-
Absorption, fluorescence emission and excitation spectra of five pigment - protein complexes were determined. photosynthetic electron transfer was measured from the dcip photoreduction. p700 concentration was assayed from the ferricyanide - oxdised minus ascorbate - reduced difference spectrum
測定裙帶菜各色素蛋白復合物的吸收光譜、熒光發射光譜和熒光激發光譜，並進行了dcip的光還原測定和化學法的氧化還原差示光譜測定。 -
Whole cell c2d2 reduction by all four mutants comparing to wild type and ni / v mutant was also detected. the result showed that only single a - gln194 substitution did not perturb the stereospecificity of protonation of c2d2. the above comparing results indicate that in mofe protein ( 1 ) a a - gln190 site and its association with homocitrate are important for the transfer of electron / proton to femoco, while a - his194 site and the homocitrate are independent in h2 evolution
對四個突變株細胞的c _ 2d _ 2還原特性及還原產物中反式-順式- 1 ， 2 -二氘代乙炔的比例進行了測定並與野生型及nifv突變株相比較，結果表明只有- gln ~ （ 194 ）替換不影響c _ 2d _ 2還原產物中反式-順式- 1 ， 2 -二氘代乙炔的比例，即未改變固氮酶還原c _ 2h _ 2加氫的立體構型的專一性。 -
Now it has been one of the most important aquatic products in the freshwater cultivation. however, this prawn ca n ' t survive at a water temperature lower than 14c, which has seriously limited its cultivation expanding. in order to obta in a new breed of this prawn with increased cold - resistance, we investigated the cloning of a synthetic gene ( sbwafp ) based on the primary sequence of the mature spruce budworm ( choristoneura fumiferana ) antifreeze protein ( sbwafp ) and the integration of sbwafp into the embryo genomes of giant freshwater prawn by spermatophore - microinjection ( smi ), a sperm - mediated gene transfer technique
本研究的特色和創新之處在於，針對羅氏沼蝦不耐低溫，但體型相對較大，精莢明顯的特點，首次將目前已知具有最強抗凍活性的雲杉卷葉蛾（ sprucebudworm ， choristoneurafumiferana ）抗凍蛋白（ sbwafp ）基因（ sbwafp ） ，通過精子介導的轉基因技術整合到羅氏沼蝦的胚胎中，以期培育出耐低溫的羅氏沼蝦新品系。 -
This sequence emergences fourteen times from 1000 ests library indicts that it is a middle affluently gene in cdna library. the cdna of 634 basepairs contains an open reading frame of 339 nucleotides encoding a novel nonspecific lipid transfer protein. the first 23 amino acids constitute the putative signal peptide, characteristic for targeting to the secretory pathway
測得th - nsltp序列全長為634bp ，含有一個非特異性脂轉移蛋白與植物耐逆性的相關性研究編碼112個氨基酸的閱讀框架， n端的23個氨基酸組成一段信號肽序列，表明它可能和分泌有關。 -
Get the inclusion bodies 2 ) western blot analysis of fusion protein expression ( 1 ) electrophoresis ( 2 ) transfer proteins from gel to membrane ( 3 ) blocking ( 4 ) incubation with primary antibody ( 5 ) enzyme conjugate incubation ( 6 ) substrate incubation. 3 ) gst detection module with cdnb enzymatic assay 3 purification of gst fusion proteins 1 the denaturalization of inclusion bodies. 2 purification using glutathione sepharose 4b column wash matrix with 1 pbs, prepare a 50 % slurry for batch purification method, pack column with matrix slurry
三、 gst一hbrp重組蛋白的純化1 .超聲破碎細胞，離心，上清和沉澱進行sds一page電泳分析2 .樣品處理提取包涵體，變性后，加人用pbs平衡過的以utal腸onesepb抓脫4b ，室溫下孵育3 .以utadtionesepharose4b柱純化以utad雲onesepharose4b柱的準備根據毛山lel ，決定純化所需的gll衛ta1如oneseph娜se4b的柱床體積，用預冷的1xpbs清洗cldtathionese戶， se4b ，得到50 %的基質 -
Functions : this product contains “ antifungal active protein ” with optimal and strong sterilization effect, which could strengthen the immunity of body fluid, activate the macrophage, strengthen the phagocytosis ability of macrophage, strengthen the body immunity, restrain the growth, pervasion and transfer of abnormal cells, the product has strong sterilization effect and could strengthen the disease resistance ; it is remarkably effective in improving the immunity and improving the infirm constitution of pets ; the amino acid content and composition in the product are moderate and rational, with the characteristics of strong palatability, nutrition balance and immune element abundance, etc
功能：本產品中擁有極佳強烈殺菌作用的「抗菌活性蛋白」 ，能增強體液免疫功能，活化巨噬細胞，增強其吞噬能力，可增強機體免疫力，抑制非正常細胞生長、擴散和轉移，具有強烈的殺毒作用，增強抗病性；對提高寵物免疫力，改善虛弱體質有顯著效果；其中氨基酸含量適中、組成合理，具有適口性強、營養均衡和免疫物質豐富等特點。 -
Association between insulin resistance and cholesteryl ester transfer protein gene polymorphism in type 2 diabetes mellitus
2型糖尿病胰島素抵抗和膽固醇酯轉運蛋白基因多態性的關聯研究 -
Relationship between endogenous estrogen concentrations and serum cholesterol ester transfer protein concentrations in chinese women
絕經后婦女雌激素缺乏與膽固醇酯轉運蛋白水平改變的關系 -
Besides, 5 % methanol enhanced the resistance to freezing stress in the sensitive species, and this effect seems to be related to maintenance of the psi activity and energy transfer between pigment protein complexes by methanol
值得注意的是5甲醇提高了非抗凍型品種synechocystissp . pcc6803的凍結耐性。其作用機制可能是在凍結脅迫下保持ps活性和色素蛋白復合體之間的能量傳遞。 -
Production of transgenic embryos expressing green fluorescent protein by nuclear transfer from different types of somatic cells in pig
不同類型的轉基因細胞為核供體生產豬的轉綠色熒光蛋白基因克隆胚胎 -
Current thoughts on the phosphatidylinositol transfer protein family
磷脂酰肌醇轉移蛋白質家族的研究進展 -
With the procedures, the overall recovery of enzymatic activity reached 20 % and the specific activity for substrate hydantoin was about 4 u / mg protein. the purification factor was about 4. 7 folds with the purity about more than 95 % as estimated by sds - page analysis. d - hydantoinase gene from strain ss - ori was cloned to five different vectors to be five recombinant plasmids and in turn to transfer into five different e. coli strains, respectively
對其中產酶活性最高的一工程菌pexsec一hdt /雲coljblz ] （ de3 ）海因酶的表達條件進行了研究，目的蛋白的表達量約占總菌蛋白的20 % ，其酶活力為0 . 92u / ml ，約是原始菌株的d一海因酶表觀活性的4 . 6倍。 -
Progress in the studies of lipid - transfer protein
植物脂質轉運蛋白的研究進展 -
Coding mechanics of a hh neuron, electron transfer in a dna molecule, protein analysis, heartbeatgait time series
序列分析、神經元等細胞內信息編碼機制、 dna分子內電子傳輸、蛋白質分析、心律步伐序列分析等的應用。 -
In order to get the soluble recombinant eo protein and inspect the protein expression status convinently, the egfp and eo gene were ligated into baculovirus transfer vector. with the co - transfecting sf9 cells of baculovirus recombinant transfer vector and linearized viral dna, and plaque purification in the posttransfection procedure, the pure recombinant baculovirus were harvested, which infected the sf9 cells for amplifying to generate a p - l stock. in the meantime, the fluorescence microscopy detection indicated expressed egfp protein to confirm the heterogenous protein expression of recombinant baculovirus. the pi - stock from a pure plaque was used to generate a high liter p - 2 stock, which was determined in liter as 1. 14 107pfu / ml by performing a plaque assay. when a volume of p - 2 stock infected the sf9 cells with moi 5 - 10 for expression, the strong fluorescence was obeserved on the day 3 of postinfection
此外，為了得到可溶性重組eo蛋白並便於觀察重組蛋白的表達情況，我們將egfp基因與eo基因相連插入昆蟲桿狀病毒轉移載體中，與線性桿狀病毒dna共轉染sf9細胞后通過噬斑純化得到純的重組桿狀病毒，將其感染sf9細胞制備p1種子液，同時用熒光顯微鏡觀察綠色熒光蛋白的表達情況剔除表達效果差的重組桿狀病毒。再用p1種子液感染sf9細胞制備高效價的p2種子液。通過病毒液的梯度稀釋和噬斑測定，確定p2種子液的病毒滴度達1 . 14 10 ~ 7pfu ml 。 -
Cloning and expression of cdna for maize nonspecific lipid transfer protein as well as calmodulin - binding activity analysis of the expression product
一種新的煙草鈣調素結合蛋白的分離鑒定 -
Reverse northern hybridization indicated that p1a5 gene was over - expressed in bract compared with that in leaf. this expression pattern was further conformed by rt - pcr. sequence analyses revealed that it is a partial sequence of a lipid transfer protein gene
通過抑制性差減雜交的到克隆pias ，反向northern雜交表明它在玖桐苞片中優勢表達，即在苞片中的表達水平遠遠超過在處于同時期的葉片中的表達水平。 -
The recombinant pcr technic was used to introduce a linking peptide klgggg to the site between scfv single chain form of the monoclonal antibody sz - 51 specific for the glycoprotein gmp140 on activated platelet membrane and uk32 low molecular weight form of pro - urokinase, to make the scfv - linker - uk32 chimeric gene. this gene was cloned into the transfer vector pbacpak9, and cotransfected with bacpak6 bsu36i digest into sf 9 cells. the fusion protein was secreted into the medium. in the fifth day after the cotransfection, the supernatant of the medium showed 107 iu ml fibrinolytic activity, higher than 25 iu ml fibrinolytic activity of scfv - uk32. elisa showed that the supernatant had the binding activity to activated platelet. wastern blotting also indicated that the supernatant could bind to the monoclonal antibody of urokinase b chain
為了提高重組導向溶栓分子scfv - uk32的溶纖活性，通過重組pcr方法在編碼scfv與uk32的堿基之間引入編碼klgggg連接肽的堿基序列，並克隆到轉移載體pbacpak9上，通過與線性病毒dna bacpak6 bsu36i digest共轉染到昆蟲細胞sf 9內，進行表達。表達產物分泌到上清中，共轉染后第5d天用纖維平板法測得sf 9細胞上清溶纖活性達到107 iu ml ，比未引入連接肽的scfv - uk32的表達活性25 iu ml高。 -
The deduced amino acid sequence of p1a5 was most homologous to the lipid transfer protein 3 precursor isolated from upland cotton, the lipid transfer protein sdi - 9 isolated from common sunflower, and the nonspecific lipid - transfer protein precursor allergen pru av3 isolated from sweet cherry
對得到的該片段分析表明，它是一種脂肪轉移蛋白（ ltp ）基因的部分序列。採用5 』端race技術獲得了該基因的全長cdna序列n 。 -
Gene population encoding proteins that involve in plant defensive reactions and adversity resistance, such as p1a5 gene encoding a non - specific lipid - transfer protein, p1b4 gene encoding a raucaffricine - o - p - d - glucosidase and p2d4, encoding a proline - rich protein ; ( 2 ) gene populations with antagonistic effects occurred simultaneously in sprouting bract, e. g.,
如pib4基因編碼的葡萄糖昔酶、 pias基因編碼的植物脂肪轉移蛋白、 pzd4基因編碼的一種富含脯氨酸的蛋白都有參與植物的抗逆、防禦或應激反應的功能八2 ）幼嫩苞片中有互為桔抗作用的基因同期表達。
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