transforming sequence 中文意思是什麼

transforming sequence 解釋
轉化序列,轉化順序
  • transforming : 重排
  • sequence : n 1 繼續；接續；連續。2 順序；程序；次第；關系；關聯。3 後果；結果；接著發生的事；後事；後文。4 ...
  1. In this paper, first strand cdna of 3abc gene was synthesized using template rna extracted from cells infected with fmdv. the complete 3abc gene about isoobp was amplified by pcr and ligated into pgem - t easy vector. after transforming e. coli dh5 a, ampicillin resistant colonies were isolated and plasmid dna was prepared and analyzed by restriction analysis and pcr. presence of the full length 3abc gene was verified by nucleotide sequence analysis and the plasmid containing the expected sequence was named as pgem - 3abc. comparing the aquired sequence of 3abc with that of reference strains, the homology is more than 99 percent. the pgem - 3abc was digested with sal i and bgl ii and ligated into xho i and bgl ii - digested expression vector ptriex - 4 neo. lt was identified by restriction analysis and pcr and sequencing that this fragment had a 17bp deletion hi the nucleotide sequence 708bp of 3abc gene, which happened to form a terminator codon behind 3ab gene, but it contained the complete open reading frame ( orf ) of 3ab gene. positive clones were selected and induced with lmmol / l isopropyl - d - galactoside ( iptg ), bacteria were detected by sds - page and western blotting after properly treated. the results showed that the 3ab gene expressed successfully in e. coli and 33. 5ku fusion protein can be recognized by the positive bovine serum of fmdv. the amount of target protein is over 26 % of the total bacteria protein by gel thin layer scanning analysis

    擴增產物連接到pgem - teasy載體中，轉化大腸桿菌dh5菌株，篩選氨芐青霉素抗性菌落，提取質粒經酶切鑒定、 pcr分析以及確證性測序證明，所克隆的1500bp左右的片段含有完整的3abc基因，與國外參考序列相比，同源性在99以上。將重組質粒pgem - 3abc和表達載體ptriex - 4neo分別用sal和bgl與xho和bgl消化后，亞克隆3abc基因至原核表達載體ptriex - 4neo中，通過酶切鑒定、 pcr擴增以及序列分析，發現克隆到ptriex - 4neo載體上的片段於3abc基因708bp處出現了17bp的缺失，碰巧在3ab基因后形成一終止密碼子，但3ab基因的閱讀框架完整，選出含有3ab基因完整閱讀框架的陽性克隆，用iptg誘導表達，收集菌液進行sds - page電泳、 westernblotting分析，結果表明， 3ab基因在大腸桿菌中成功表達，其表達產物為分子量33 . 5ku的融合蛋白，並能被口蹄疫病毒陽性血清識別。經薄層掃描分析，表達量占總蛋白量的26以上。
  2. The experiment results indicated that conditional on transforming layer 1mm thick and absorbing layer 2mm thick, the combined materials which were processed by 600 and 800 respectively have good absorbing performance, and the influence of the combing sequence on absorbing performance is indistinctive

    實驗表明，當變換層為1mm ，吸收層為2mm時，以600處理的和800處理的材料進行不同組合都有較好的吸波性能。
  3. Researched four technology of structure conversion about digital video, de - interlaced, frame ratio down or up, row ration down or up, the width and high transforming, implemented lots of corresponding arithmetic of four techniques, motion compensate structure conversion method based on the motion estimating improved the image sequence definition and liquidity degree

    4研究了數字視頻結構變換的四種技術：去隔行技術，變頻技術，行頻轉換技術，寬高比變換技術。實驗驗證了大量的相關演算法，其中基於運動估計的運動補償結構轉換方法，清晰度和流暢度得到了很大的提高。
  4. After that comes a sequence of transforming rules

    之後是一系列轉換規則。
  5. Scientists have completed the finished sequence of the human genome, or genetic blueprint of life, which holds the keys to transforming medicine and understanding disease

    科學家完成了人類基因的最後排序，即生命藍圖的工作，這對於人類醫藥產業和研究疾病有巨大的幫助。
  6. Among the algorithmic design, the corresponding solution to the problems of transforming from the real sequence to binary sequence and precision in the chaotic stream cipher is put forward, and more efficient algorithm and better randomness of key sequence are obtained

    在演算法的設計中，我們對混沌序列密碼所面臨的實數序列到二進制序列的轉化和精度等問題採取了相應的解決方法，從而獲得較高的演算法執行效率和良好的密鑰序列隨機性。
  7. The signal peptide sequence which was used for secretedly expressing divergent gene in mammary gland cells was ligated to the e2 gene, the e2 gene with signal sequence was obtained by pcr. the gene resisting kanamycin was cut down from pgfp - cl vector and inserted into p22 vector, a p22 vector with gene resisting kanamycin was constructed, it was tried to construct an expression vector for transforming go

    豬瘟病毒ez基因的乳腺特異表達載體構建：將在乳腺細胞中特異分泌的信號肽序列連接到ez基因， pcr得到了目的片段，再將pgfpci載體上的kana基因切下與在乳腺細胞中特異表達的p22載體連接，使其帶有篩選標記，然後將帶有信號肽的ez插入到p22中，試圖構建山羊乳腺上皮細胞的特異性表達載體。
  8. According to the present actual underground power system which neutral point is grounded through arc suppression coil in many supper big shaft, the development history and present situation of synthetic protection system used in hv flameproof switchgear are reviewed, and on the basis of analyzing the problems existed in high voltage flameproof switchgear, the selective leakage protective method based on the detecting harmonic direction of zero sequence current as well as the value of zero sequence voltage is proposed in this paper, and at the same time, harmonics extracting schematic and transforming circuit are also designed

    本文深入系統地研究了基於intel80c196kb單片機控制的6kv防爆開關綜合保護系統，主要內容如下：針對大型及特大型礦井供電系統中性點普遍採用消弧線圈接地方式的現狀，回顧了國內外礦用高壓防爆開關保護系統的發展歷史，分析了目前國內高壓饋電開關保護系統所存在的問題，提出了「基於零序電壓基波啟動，五次諧波功率方向比較」的選擇性漏電保護原理，設計了相應的五次諧波提取電路和功率方向鑒別電路。
  9. An optimizing method based on biological evolution - genetic algorithm is proposed to the problem of the construction process planning analysis. adapting the successful experience of path optimization problem, improved genetic operators are adopted : the sequence of numbers, which is arranged by the removal order of members, is assumed to represent a chromosome by natural code ; transforming baseline information of this problem into immune operators ; combining rank - based model with elitist model ; designing special crossover and mutation operators. the mathematical model for computer and special programs are provided in this paper

    借鑒遺傳演算法在求解路徑優化問題上的成功經驗，應用了改進的遺傳運算元：採用自然數對結構去除構件進行編碼，將所求問題的基本信息轉換成免疫運算元，採用穩態最優保存策略和排序選擇方法相結合進行選擇，採用適用於本問題研究的特殊的交叉運算元和變異運算元，從而來討論在桁架結構施工路徑優化問題中使用遺傳演算法的可能性。
  10. The combination absorbs microwave well with the least reflectivity of - 31. 59db, and minimally having 2. 31ghz - frequency band. these results illuminated the directional law of combined materials, it is that, when the double deck with the different thickness, no matter what is absorbing or transforming layers, its absorbing performance would not be influenced on the whole. when the double deck with the same thickness, the combining sequence of materials would cause crucial influence on its absorbing performance

    在8 . 2 12 . 4ghz頻段內，最小反射率達- 45 . 93db ，有效頻寬為4 . 0ghz ；當變換層和吸收層厚度相同，總厚度為2mm時，要獲得較好的吸波性能，必須以高溫處理的材料作變換層，低溫處理的材料作吸收層，即存在材料組合的方向性，其中以1000分別與600 、 700處理的材料組合性能最佳，其最小反射率為- 31 . 59db ，有效頻寬最小為2 . 31ghz 。
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