transgenic fish 中文意思是什麼

transgenic fish 解釋
轉基因魚
  • transgenic : adj. 【生物化學】轉基因的，（物種）通過轉基因培育的。
  • fish : n 菲什〈姓氏〉。n （pl fishes 〈集合詞〉 fish）1 魚；〈集合詞〉魚類；魚肉。 ★說魚的若干種類時用 f...
  1. The research adopts that hu - - ifn gene were introduced into the nuclei of oocytes or cytoplasm of grass carp to develop anti - disease transgenic grass carp breeding researches, combing the adva ntag e of hu - - ifn gene and breeding by genetic engineering, with an aim of finding out an effective way of solving antivirus of hemorrhagic virus of carp completely. in research of transgenic fish, hu - - ifn gene ( recombination gene ) is cutdown and introduced into the nuclei of oocytes or cytoplasm of grass carp at one - cell or two - cell stage via micyoinjection by narashige micyoinjection apparatus

    本研究的目的在於以人的-干擾素基因（ ifn - ）作為目的基因，與鯉魚-肌動蛋白基因啟動子在體外重組，利用原核顯微注射轉基因技術將人-干擾素基因導入草魚基因組而開展的抗病轉基因草魚育種研究，其結合了干擾素和基因工程育種抗草魚出血病病毒的優點，以期獲得對草魚出血病具有天然抗性的轉基因魚，並在此基礎上培育出草魚抗病新品系。
  2. In the research of transgenic fish, green fluorescent protein gene was sub - cloned to downstream of carp p - actin gene promoter that was cloned in pucusa by molecular recombination technology. thus pagfp plasmid was constructed successfully. the recombination was determined by digestion of restriction enzyme and sequencing

    實驗通過分子重組技術，採用定向克隆法將綠色熒光蛋白基因亞克隆到puc118a上鯉魚-肌動蛋白基因啟動子下游，構建成能在真核生物體內表達的表達載體pagfp ，經雙酶酶切法序列鑒定后，回收帶啟動子和目的基因片段。
  3. They cannot naturally produce essential fatty acids - beneficial nutrients found mainly in plant and fish oil, so they must rely on a dietary supply. here we want to establish transgenic mice carry a tissue - specific expression gossypium hirsutum - 6 fatty acid desaturase ( fad2 ) gene which can add a double bond into an monosaturated fatty - acid hydrocarbon chain and convert oleic acid to linoleic

    本試驗試圖構建一個肌肉組織特性的- 6脂肪酸去飽和酶（脫氫酶）真核表達載體，通過原核顯微注射法把棉花的- 6脂肪酸去飽和酶導入到小鼠體內，以小鼠為動物模型，建立動物體內的必需脂肪酸合成代謝通路。
  4. The rapid development of modem molecular biology technique suppies a possible and efficient way for us to solove the problem completely with a comprehensive researches results to transgenic fish in recent years both domestic and aboard, it proves that it " s possible and makes great senses to cultivate a high quality fish to meet the satisifaction of the people

    現代分子生物學技術突飛猛進的發展為我們切底解決這一難題提供了可能性和有效途徑。綜觀國內外近些年來對轉基因魚的研究及結果證明，通過轉基因定向培育出符合人們所需要的優良魚種是完全可能的，也是十分有意義的。
  5. The transgenic goldfish were detected and screened by pcr, southern blot. the results showed that pcr amplify a 478bp specific molecular band in 5 individuals, southern blot results showed a strong signal in transgenic fish

    Pcr檢測結果顯示，有5尾轉化個體的總dna能擴增出一條478bp的特異性分子帶，說明轉化個體整合有gfp基因，整合率為42 。
  6. The results showed that pcr applify a 485 specific molecular band in 672 individuals, the rate of positive reaction is 60 %, southern blot result shows a strong signal in transgenic fish. it is concluded that hu - - ifn gene has been integrated and expression. it is also that foreign gene integrating position and copy number is different individuals, by elis a detecting, 23. 5 % transgenic fish " were detected hu - - ifn gene expression in all 672 transgenic fish, but expression level of hu - - dfn is significant difference in different individuals and growth period

    2000年共顯微注射32197粒草魚受精卵，出苗12945尾草魚魚苗，經孵化培育最後獲得1120尾五寸左右草魚，對1120尾草魚提取血液總dna進行pcr和southern雜交檢測， 2000年轉導的草魚的pcr檢測結果，有672尾草魚即600k轉化個體的總dna能擴增出一條485kb的特異分子帶，說明轉化個體整合有huj ifn基因，經southern雜交進一步證實，轉入的抗病相關基因己在轉基因草魚體內染色體上得以整合，但整合位點沒有固定區域，整合的拷貝數存在較大差異。
  7. It is concluded that " green fluorescent protein gene has been integrated and expressed in at least 2 transgenic fish, it is also showed that foreign gene integrating positions and copy numbers are differ in different individuals

    對于基因組上整合有gfp基因的2尾轉化個體， gfp基因在基因組上的整合位點和拷貝數並不相同，說明gfp基因作為外源基因在轉基因個體上的整合位點和拷貝數是非確定性的。
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