transient expression 中文意思是什麼

transient expression 解釋
瞬時表達,短暫表達
  • transient : adj 短暫的，一時的；過渡的；匆匆而過的；易逝的；虛幻的，無常的 （opp lasting permanent）; 【物理...
  • expression : n 1 表現，表示，表達。2 詞句；語句，措辭，說法。3 表情，臉色，態度；腔調，聲調。4 【數學】式，符...
  1. The transient expression of mgfp4 in rice calli

    4在水稻愈傷組織中的瞬時表達
  2. We successfully construct the eukaryotic expression vector of gfp - eif - 5a and its mutational vector using genetic engineering techniques. we found that eef - 5 a localized in nucleus as well as in cytoplasm just for a short time after its transient expression, then distributed only in cytoplasm

    Eif - 5a的hypusine修飾是其活性和功能發揮所必需的，我們通過pcr方法實現了hypusine位點的定點突變，並進一步構建了含gfp標簽的eif - 5a及其hypusine位點突變的真核表達載體。
  3. The expression efficiency difference between ped5 and pcdhfrl, a vector utilizing cmv enhancer / promoter ( pcmv - ie ) for foreign protein production, was analyzed using human interferon - p ( ifn - ) gene and human secreted alkaline phosphatase ( seap ) gene as reporters. when analyzed in transient expression, ped5 showed a little more protein produciton than pcdhfrl. however, in continuous expression, when serum concentration was lessened to slow down cell proliferation, ped5 expressed 3. 1 times more reporter proteins than pcdhfrl, which implied that pef - io was less affected by cell cycle status in contrast to pcmv - ie, making ped5 a good expression vector for foreign protein production

    應用人-干擾素（ ifn - ）和人分泌型堿性磷酸酶（ seap ）基因作為報告基因，對含有巨細胞病毒即早期啟動子（ p _ （ cmv - ie ） ）的表達載體pcdhfr1和ped5表達外源蛋白的能力進行了比較，發現對于瞬時表達， ped5略好於pcdhfr1 ；在穩定表達中，通過降低血清濃度，使細胞增殖緩慢，這時ped5表達外源蛋白的能力較pcdhfr1高3 . 1倍。
  4. Transient expression of fibrillin promoter in protoplasts of arabidopsis thaliana and brassica napus

    操縱子在甘藍型油菜和擬南芥中的瞬時表達
  5. The transient expression of lacz driven by crtw 306bp s ' - flanking sequence and crtz 302bp s ' - flanking sequence shows that these two sequences habour transcription regulatory sequences

    以lacz為報告基因的瞬間表達實驗結果表明，長度分別為306bp和302bp的crtw和crtz5 '上游側翼序列具有很強的啟動轉錄活性，提示兩段序列包含了啟動子的結構。
  6. To identify its laticifer - expression character, two transient - expression vectors ( pirl, pbir2 ) were constructed. in the transient - expression vector, ref promoter sequence replaced camv35s promoter of pbi121

    為驗證其乳管表達活性，通過取代pbi121中間表達載體上的camv35s啟動子，構建了兩個含gus基因的瞬時表達載體pbir1 、 pbir2 。
  7. The main factors influencing agrobacterium - mediated genetic transformation of portulaca grandiflora hook, are as follows. the concentration of kanamycin of devision leaves and the calli were 100mg / l and 200mg / l, respectively. calli infection can gain more gus gene transient expression and resistant tissuses than devision leaves

    對松葉牡丹進行遺傳轉化研究發現，採用葉片作為外植體時， kan的濃度100mg / l就可抑制愈傷的誘導，而用愈傷組織作為材料感染時濃度要達到200mg / l才可。
  8. In this paper, the repairable queuing system with police is studied. some important queuing quantities and reliability quantities are in detail discussed as follows : ( 1 ) using the renewal process theory and the method of stochastic decomposition, both the transient and equilibrium distributions of the queue length from any state are discussed, and the recursion expression of the equilibrium distribution is given by furthermore, the generating function of equilibrium queuing length distribution is given by ( 2 ) the distribution of waiting time is discussed, and the mean waiting time is given by ( 3 ) the departure of the system is discussed, and the mean value of departure customers when is given by

    具體如下： （ 1 ）利用更新過程理論和隨機分解方法，討論了從任意初始狀態出發隊長分佈的瞬態解和穩態解，得到了隊長平穩分佈的遞推公式：並進一步求得了系統的穩態隊長分佈的母函數（ 2 ）討論了穩態等待時間分佈，求得了平均等待時間： （ 3 ）討論了系統的輸出過程，求得了長期單位時間內離去顧客的平均數： （ 4 ）討論了服務臺的不可用度，得到了穩態不可用度: （ 5 ）討論了內服務臺平均故障次數，求得了長期單位時間內的平均故障次數
  9. ( 3 ) on the basis of the deletion analysis, three substitution mutants ( ml : 6bp sequence upstream of gcc box m2 : gcc box and m3 : g box - like sequence ) by pcr were designed to isolate the essential ja - responsive element. transgenic tobacco plants containing promoter substitution constructs were generated by agrobacterium - rnqdiaied leaf transformation. loss - of - function experiment, using transient expression analysis of gus reporter genes, confirmed that gcc box act as an essential element to respond ja signaling in pdf1. 2 promoter

    （ 3 ）在缺失突變的基礎上，通過對gccbox及其相鄰的上下游六個堿基進行取代突變，將突變啟動子與gus構建融合基因，在煙草中受heja誘導的瞬時表達結果表明， h1和m3的突變對該啟動子應答ja信號的影響很小，而m2 （ gccbox的突變）則幾乎使該啟動子應答ja信號的功能完全喪失，所以gccbox是該啟動子中應答ja信號的必需元件。
  10. Effect of pacap on the activation of icam - 1 and the expression of inflamnatory cytokine in the cerebral cortex during transient focal cerebral ischemia - reberfusion in rats

    垂體腺苷酸環化酶激活肽對大鼠腦缺血再灌注后細胞間黏附分子表達的影響
  11. Expression of transient receptor potential channel 4 in striatum and hippocampus of rats is increased after focal cerebral ischemia

    大鼠局灶性腦缺血再灌注損傷后紋狀體和海馬區瞬時受體電位通道蛋白4的表達增加
  12. Acetosyringone ( as ) can increase the gus gene transient expression when it was added in the suspending medium

    在菌液中添加as可以提高gus基因的瞬時表達。
  13. The results were that transient expression had not obtained after transformating dry seeds implanted

    結果表明：直接注入干種子后進行轉化，未得到瞬間表達的結果。
  14. Sonication - assisted agrobacterium - mediated transformation ( saat ) can increase the efficiency of transformation. 2s sonication can enhance the gus gene transient expression and induction of resistant buds without leaves incision

    頭孢毒素（ cef ）對不定芽的誘導有一定的抑制作用，哌拉西林鈉（ pip ）對不定芽的誘導抑制作用稍弱，以pip400mg / l作為抑菌生長濃度。
  15. But if 2 - day - seedling was implanted, and then dipped into dna solution, the transformation effect was increased evidently. 10 % transient expression was obtainted

    而注入玻璃化保護后的2天齡幼苗，再進行轉化，轉化效果明顯提高，得到了大約10 %的瞬間表達。
  16. The expression of scfvs fusion protein were detectable by fluorescence microscope directly and indirect immunofluorescence and immunohistochemistry analysis after transient expression in cos - 7

    瞬時轉染cos刁細胞后，通過熒光顯微鏡觀察、間接免疫熒光檢測、免疫組化檢測證實了scfv融合蛋白的表達。
  17. In transgenic tobacco plants, the analysis of transient expression by monitoring 3 - glucuronidase activity revealed that a chimeric gene construct containing a 1. 2 kb pdf1. 2 promoter fused to a gus reporter gene was induced by meja

    （ 1 ）從擬南芥基因組中擴增出長度約為1200bp的pdf1 . 2啟動子，與gus構建的融合基因在煙草中的表達受meja誘導。
  18. In transgenic tobacco plants, the transient - expression assay of the chimeric gene ( 4 x gcc - 35s min : : gus ) demonstrated that the 4 x gcc - 35smin promoter could respond to meja treatment and the gcc box is an important element in response to ja signaling. moreover, this experiment results would be meaningful to improve the crops characterization of resistance against various environment stresses or to study the regulation of gene expression in transgenic plants

    （ 3 ）以反向的4xgcc重復序列（ placzi 4xgcc （ 》作為placzi4xgcc的突變體， 6半乳糖苦酶活性分析的結果表明，與野生型的相比，突變的gcc元件不能與jerfi 2 3 4相互作用， p半乳糖苦酶實驗不能呈現出藍色反應；證明gccbox與jerf 2 3 4是特異性結合。
分享友人
分享到 Line

分享到臉書