tricine 中文意思是什麼

tricine 解釋
兩性離子緩沖劑
  1. Two protein peaks can be obtained by bio - gel p - 6 chromatography and both peaks have antimicrobial activity. so the bacteriocin is consisted of two proteins with different mw. only one protein with larger mw can be detected through tricine - sds - page, and its mw is about 8, 570da

    採用30硫酸銨就能完全把發酵液中的細菌素全部沉澱，通過生物膠bio - gelp - 6層析發現細菌素被分離出兩條抗菌蛋白峰，這表明r21 - 4產生的細菌素是由兩種不同分子量的蛋白質組成的，通過tricine - sds - page檢測，只能檢測到一條分子量相對較大的細菌素，分子量在8 ， 570da左右。
  2. Strain bl21, and gene expression was induced by iptg. the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene. using ion exchange chromatography and gel filtration chromatography, the chimera proteins were purified, and both of them showed a single band in tricine - sds - page. the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities, but gain platelet aggregation inhibitory activities as well

    通過離子交換層析和凝膠過濾層分別對兩種嵌合體蛋白進行純化，純化產物在tricine - sds - page中都顯示為單一條帶。活性分析結果表明兩種嵌合體蛋白在保留水蛭素抗凝血酶活力的同時，還呈現抗血小板聚集活性。
  3. The hwtx - i gene was chemically synthesized according to its known cdna sequence, the gene was inserted into vector ppic9k which contained aoxj promotor and the sequence of a secreting signal peptide - a - factor, the cloning ppic9k / hwtx - i was constructed and confirmed by two - step pcr and dna sequence analysis, then it was transformed into host strain gs115, a his + muts cell line was screened and multicopy transformants were screened by various g418 concentrations, the multicopy transformant was named gh1. gh1 was cultivated in flasks. after 6 days of induction by 0. 5 % methanol, the supernatant was checked by 16. 5 % tricine - sds page, which showed there was a band in the position of 3. 5 - 6. 1kd, then it was isolated and desalted by ultrofiltration followed by ion exchange of cm column, after reverse phase hplc of ci8 and vacuum drying, the purified rhwtx - 1 was obtained which was proved to be correct recombinant hwtx - i by tricine sds - page, maldi - tof mass spectrometry, amino acid composition analysis, the n - terminal amino acid sequence and its biological activity, the final field of the purified rhwtx - i was about 80mg / l, accounting for 23. 6 % of it total secretory proteins

    將帶有hwtx -基因的ppic9k經blgii線性化后，轉化酵母宿主菌gs115原生質體后經篩選陽性克隆並經表型鑒定為his ~ + mut ~ s酵母菌，進一步用遺傳毒素g418篩選多拷貝的轉化菌株，命名為gh1 ；將gh1甲醇酵母菌用0 . 5的甲醇誘導表達，發酵上清經90飽和度的（ nh _ 4 ） _ 2so _ 4沉澱， yw - 3 （ mwc03000 ）的超濾膜超濾，再經cm陽離子交換， c _ （ 18 ）反相hplc純化得到分子量為4kd左右的組分，其中4289 . 05的組分經質譜鑒定，氨基酸組成分析和序列測定為正確的表達產物，生物學活性表明其活性為天然毒素活性70 % ，表達量為80mg / l 。
  4. Then bacteria were collected by centrifugation and split by ultrasonic method. the purified products were analyzed by tris - tricine sds - page. the immuno - activity of the recombinant proteins were analyzed by western blot

    將離心收集到的菌體超聲破壁，離心分離，收集上清液，進行離子交換層析和分子篩分離純化。
  5. Methods and results : proteomics approaches involve three critical techniques : two dimensional electrophoresis, biological mass spectrometry and bioinformatics. in 2 - de, we applied traditional ief in the first dimension and tris - tricine system in the second dimension and acquired protein profiles of serum and skin samples. in the identification of 10 proteins of serum and 16 proteins of skin, we acquired peptide mass of fingerprint ( pmf ) maps for all targets by means of matrix - assisted laser desorption - ionization time of flight mass spectrometry ( maldi - tof - ms ) and partial aminio acid sequences for 9 proteins by means of electrospray ionization ms / ms ( lisi - ms / ms )

    研究方法：採用雙向電泳技術獲取泥鰍創傷前後血清和皮膚的小分子蛋白圖譜，利用基質輔助激光解析飛行時間檢測質譜分析（ matrix - assistedlaserdesorption - ionizationtimeofflightmassspectrometry ， maldi - tof - ms ）和電噴霧離子化串連質譜分析（ electrosprayionizationms / ms ， esi - ms hs ）分別獲得差異蛋白點的肽指紋圖譜（ peptidemassoffingerprint ， pmf ）和部分序列信息，通過網際網路上的expasy服務器中和ncbi的相關軟體將這些信息和swissprot數據庫進行匹配鑒定蛋白質種類。
  6. Gene had been successfully transformed and tris - tricine - page revealed that

    ，將表達載體轉化到酵母saccharomyces cerevisiae中， southern雜交證明
  7. The peptides were extracted, and analyzed using an improved tricine sodium dodecyl sulfate polyacrylamide gel electrophoresis ( tricine - sds - page ) method

    方法通過針刺感染大腸桿菌誘導家蠅各蟲態產生抗菌肽，再經研磨、離心等步驟提取這些抗菌肽，用改進的聚丙烯酰胺凝膠電泳使其分離。
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