upstream open reading frame 中文意思是什麼

upstream open reading frame 解釋
上游開放閱讀框
  • upstream : adv. 向上游，溯流。adj. 1. 溯流而上的。2. （石油工業等）上游的，和石油的勘探、開采及輸送到起運港口有關的〈石油工業此後的提煉等過程則稱為下游（downstream）產業〉。
  • open : adj 1 開著的，開放的；可進入的，可分享的 （to）; 無蓋的，敞口的；敞開的；展開的；開的；開闊的，開...
  • reading : n 雷丁〈姓氏〉。n 1 閱讀；讀書；講讀；朗讀，講讀會，讀書會；（議會議案的）宣讀。2 讀物。3 學識；...
  • frame : n 1 機構；組織；系統。2 結構，框架，構架，骨架，骨骼。3 體格，身軀。 4 精神狀態，心情。5 【園藝】...
  1. The tk gene contained an open reading frame of 957 bp encoding a 318 aa protein. upstream of the tk orf, three putative gc boxes are located at positions - 22, - 166 and - 199. a potential poly a signal begins 110 nucleotides downstream from the termination codon at position 1306

    在tkorf上游- 22 、 - 166 、 - 199位存在3個gc框樣序列，在終止密碼子下游第110個核苷酸處的1306位存在有多聚腺苷加尾信號。
  2. The 3. 4kb ecori dna fragment of the pgxn201 and the mutant ecori dna fragments of the three mutant plasmids were subcloned into the ecori sites of the clone vectors ml3 and the pgem3z - f ( + ) respectively. through dna sequencing and dna sequence analysis by comparing with the bradyrhizobium japonicum dna sequences reported, we found that there are four intact open reading frames in the 3. 4kb fragment and it was highly homologous to the bradyrhizobium japonicum dna sequence reported, and the tn5gusa5 containing in the pgxn217 was inserted in one of the four open reading frames. this open reading frame located on about 5. 5kb upstream of the nfec gene, which includes 207bp nucleotides, coding 68 putative amino acids and this is completely identical to that of the bradyrhizobium japonicum dna sequence reported

    本研究首先利用轉座子tn5gusa5對重組質粒pgxn201進行誘變，獲得3 . 4kbecori片段插入了tn5gusa5的突變質粒pgxn215 、 pgxn216 、 pgxn217 。對這些突變質粒進行亞克隆及測序分析並與基因庫中已報道的慢生型大豆根瘤菌的dna序列作比較，發現突變質粒pgxn217中tn5gusa5恰好插入在一個未知功能的開放閱讀框架內。將pgxn201的3 . 4kbecori片段與m13作連接，獲得亞克隆pgxn201c ，對pgxn201c進行測序分析，發現與基因庫中已報道的慢生型大豆根瘤菌的dna序列有95的同源性。
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