upstream shift 中文意思是什麼
upstream shift
解釋
逆向位移-
Closely cooperates with, communicates with and supports downstream and upstream units of production in reference to the status of process and according to the instructions from shift supervisor / deputy shift supervisor
根據工藝狀態和班長/副班長指令,密切與上下游生產單元進行合作、溝通和進行支持。 -
A new e. coli promoter - probe vector phn1005 was constructed by using a red - shift enhanced gfpmut3 as reporter gene and showed the following characters : 1, bamhi at the 5 " end of gfpmut3 structure gene could be used to clone promoter - active fragment and the strength of promoter could be quantitatively assayed. 2, a rrnbtlt2 terminator from rrna gene at the 3 " end of gfpmut3 could permit clone strong promoter. 3, another rrnbt1t2 terminator was inserted upstream bamhi to prevent reading through of promoters from puc19
進一步以紅移且熒光強度提高21倍的gfpmut3為報告基因,構建了大腸桿菌啟動子探針載體phn1005 ,該載體上gfpmut3結構基因5 』端的bamhi位點可用來克隆具有啟動子活性的dna片段並定量分析插入的啟動子強度;其3 』端含rrnat1t2終止子,可允許克隆強啟動子;在bamhi上游同樣插入rrnat1t2終止子以防止載體puc19上的啟動子的轉錄通讀; gfpmut3結構基因上游還插入一段內含子序列使正反六種讀碼框的翻譯均可被終止,可保證gfpmut3以正確的讀碼框翻譯。
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