vector insect 中文意思是什麼
vector insect
解釋
病原媒介昆蟲-
The action of a pathogen and an insect vector changed the ecology of the rabbit.
病原體和昆蟲媒介改變了兔子的生態學。 -
Meq protein, highly expressed in the insect cell line sf9 by the baculovirus vector was immunized into balb / c mice and the immunized spleen cells were collected and fused with the tumor cell line sp2 / 0 via peg - 1000 in vitro. the hybridoma cells were cloned and screened for the ability of anti - meq mcab secretion by fa with the mdv ga infected chicken embryo fibroblast ( cef )
利用通過桿狀病毒載體在昆蟲細胞系sfg上高度表達的meq蛋白產物免疫balb / c小鼠，然後收獲其免疫脾細胞並與腫瘤細胞系spz / 0通過peg于體外融合;獲得的雜交瘤細胞被克隆並通過與mdv感染的雞胚成纖維細胞（ cef ）做免疫熒光試驗（ fa ） ，進行其分泌抗meq單克隆抗體（ mcab ）能力的篩選。 -
I ) two mutants ( hpab - 38 and hpab - 34 ) were designed based on the three - dimension structure of hpab - constructed by protein homology modeling method, ii ) the mutant molecules were generated by pcr and inserted into pfast hta donor plasmid, the later was then transformed into escherichia coli dhlobac to generate recombinant baculoviruses dna by site - specific transposition of an expression cassette into a baculovirus shuttle vector ( bacmid ) propagated in e. coli. the recombinant bacmids were isolated and transfected into insect sf - 9 cells to reproduce baculoviruses
4 . h隊b一p及其突變體高效表達工程菌的構建:將用pcr擴增的h隊b一p 、 h獄b一p35 、 h隊b一p34基因，分別與pinpointxa一3質粒連接，轉化jm109大腸埃希菌，獲得的工程菌在表達融合蛋白時不穩定，第5代后即見不到表達條帶。
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